B7-1/CD80 Antibody AF140 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemB7-1/CD80 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF140
ApplicationsELISA Development,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry
IsotypeIgG
HostGoat
TargetB7-1/CD80
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
ReactivityHuman
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7-1/CD80, Extracellular domain
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
NCBI Full Gene NameCD80 molecule
NCBI Gene AliasesB7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human B7-1/CD80 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human B7-2 and recombinant mouse B7-2 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) In the diffuse large B-cell lymphoma microenvironment, SIRT1 is upregulated and correlated with a pro-inflammatory macrophage signature and autophagy-related gene expressionFrontiers in ImmunologyFebruary 4, 2026Miguel Resanoa, Peio Azcoaga, Naike Salvador, Alba Delgado, Claudia Rodiño, Matthieu Schoenhals, Mounia S. BrazaAfter heat-induced epitope retrieval, we incubated TMA sections with anti-CD80 (Novus Biologicals, AF140, USA), anti-M-CSF (Novus Biologicals, AF216, USA), anti-SIRT1 (Abcam, Ab110304, UK) and anti-SIRT3 (Abcam,

(2) Tissue Levels of CD80, CD163 and CD206 and Their Ratios in Periodontal and Peri-Implant Health and DiseaseCurrent issues in molecular biologyOctober 10, 2022Yilmaz M, Demir E, Firatli Y, Firatli E, Gürsoy UK.CA, USA) and the membranes were incubated with the primary antibodies (1:500 dilution; B7-1/CD80: #AF140, CD163: #AF1607, MMR/CD206: #AF2534; R&D Systems, Bio-Techne, USA) overnight. Then, the membranes were

(3) Usefulness of the cytokines expression of Th1/Th2/Th17 and urinary CD80 excretion in adult-onset minimal change diseasePeerJJanuary 1, 2020Chen P, Chen Y, Jiang M, Mo Y, Ying H, Tang X, Zhang J.

(4) Peripheral alveolar nitric oxide concentration reflects alveolar inflammation in autoimmune pulmonary alveolar proteinosisERJ open researchJanuary 1, 2018Hirano T, Ohkouchi S, Tode N, Kobayashi M, Ono M, Satoh T, Mitsuishi Y, Watanabe A, Tabata M, Irokawa T, et al.control were adopted. For immune staining, antibodies against iNOS (ab15123; Abcam, Cambridge, UK), CD80 (AF140; R&D Systems, Minneapolis, MN, USA), CD206 (ab64693; Abcam) and 4′,6-diamidino-2-phenylindole (D1306;

(5) Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinomaOncology lettersAugust 1, 2017Ai P, Li Z, Jiang Y, Song C, Zhang L, Hu H, Wang T.from 39 NPC patients. As shown in goat anti‑human CD80 polyclonal antibody (1:100; IgG; catalog no. AF140; R&D Systems, Inc., Minneapolis, MN, USA), goat anti‑human CD86 polyclonal antibody (1:100; IgG; catalog no.

(6) The costimulatory receptor B7-1 is not induced in injured podocytesKidney InternationalNovember 1, 2016Emilie Baye, Morgan Gallazzini, Marianne Delville, Christophe Legendre, Fabiola Terzi, Guillaume Canaudinfiltrating cells (Figure 3e). Of note, because the anti-B7-1 antibodies gave similar results, only the AF140, goat antibody from R&D Systems Europe (Lille, France) is shown in Figure 3e. We therefore conclude

(7) B7-1 Blockade Does Not Improve Post-Transplant Nephrotic Syndrome Caused by Recurrent FSGSJournal of the American Society of Nephrology : JASNAugust 1, 2016Delville M, Baye E, Durrbach A, Audard V, Kofman T, Braun L, Olagne J, Nguyen C, Deschênes G, Moulin B, et al.used. (F) B7–1 immunostaining in human tonsil using three different commercially available antibodies (AF140 from R&D Systems Europe, SC-9091 from Santa Cruz Biotechnology, and MA5–15512 from Fisher Scientiﬁc).

(8) B7-1 Is Not Induced in Podocytes of Human and Experimental Diabetic NephropathyJournal of the American Society of Nephrology : JASNApril 1, 2016Gagliardini E, Novelli R, Corna D, Zoja C, Ruggiero B, Benigni A, Remuzzi G.Am Soc Nephrol 27: 999–1005, 2016 with polyclonal goat anti–human B7–1 (diluted 1:200; catalog no. AF140; R&D Systems, Minneapolis, MN), polyclonal goat anti–mouse B7–1 (1:200; catalog no. AF740; R&D Systems),

(9) Antigen presentation and MHC class II expression by human esophageal epithelial cells: role in eosinophilic esophagitisThe American journal of pathologyFebruary 1, 2011Mulder DJ, Pooni A, Mak N, Hurlbut DJ, Basta S, Justinich CJ.

(10) Urinary CD80 is elevated in minimal change disease but not in focal segmental glomerulosclerosisKidney internationalAugust 1, 2010Garin EH, Mu W, Arthur JM, Rivard CJ, Araya CE, Shimada M, Johnson RJ.

(11) HLA-B27-restricted antigen presentation by human chondrocytes to CD8+ T cells: potential contribution to local immunopathologic processes in ankylosing spondylitisArthritis and rheumatismJune 1, 2009Kuhne M, Erben U, Schulze-Tanzil G, Köhler D, Wu P, Richter FJ, John T, Radbruch A, Sieper J, Appel H.

(12) The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysmJournal of internal medicineApril 1, 2007Sakthivel P, Shively V, Kakoulidou M, Pearce W, Lefvert AK.

(13) Induction of B7-1 in podocytes is associated with nephrotic syndromeThe Journal of clinical investigationMay 1, 2004Reiser J, von Gersdorff G, Loos M, Oh J, Asanuma K, Giardino L, Rastaldi MP, Calvaresi N, Watanabe H, Schwarz K, et al.

(14) Inhibition of T-cell activation by the CTLA4-Fc Abatacept is sufficient to ameliorate proteinuric kidney diseaseAmerican journal of physiology. Renal physiologyJanuary 1, 0001"Herrera M, Söderberg M, Sabirsh A, Valastro B, Mölne J, Santamaria B, Valverde AM, Guionaud S, Heasman S, Bigley A, et al."of commercially available anti-human B7-1/CD80 antibodies was tested: 1) R&D Systems catalog number AF140, 2) Biolegend 2D10 catalog number 305202, 3) Novus Biologicals 62N3G8 catalog number NBP2-25255, and

(15) Serum from minimal change patients in relapse increases CD80 expression in cultured podocytes"Pediatric nephrology (Berlin, Germany)"January 1, 0001"Ishimoto T, Cara-Fuentes G, Wang H, Shimada M, Wasserfall CH, Winter WE, Rivard CJ, Araya CE, Saleem MA, Mathieson PW, et al."membrane was blocked with 5% non-fat milk and then incubated overnight with primary CD80 antibodies (Cat#AF140, R&D System). After washing, the membrane was incubated with a mouse anti-goat HRP-conjugated secondary

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B7-1/CD80 Antibody

B7-1/CD80 Antibody

B7-1/CD80 Antibody

B7-1/CD80 Antibody

B7-1/CD80 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (15)

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B7-1/CD80 Antibody from R&D Systems, a Bio-Techne Brand

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