Sca-1/Ly6 Antibody AF1226 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemSca-1/Ly6 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $539.00Supplier Page View Company Product Page
Catalog NumberAF1226
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,CyTOF-ready
TargetSca-1/Ly6
IsotypeIgG
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityMouse,Rat,Transgenic Mouse
HostGoat
Quantity100 ug (also 25 ug)
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Sca-1 C-terminally truncated Ly-6E allele, Leu27-Gly119, Accession # CAA28351
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namelymphocyte antigen 6 family member A
NCBI Gene AliasesLy-6A.2, Ly-6A/E, Ly-6E.1, Sca-1, Sca1, TAP

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse Sca-1/Ly6 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for Western blotting in my experiments?

Yes, the Sca-1/Ly6 antibody has been shown to work effectively in Western blotting applications, as evidenced by its use in various studies, including those that analyze active β-catenin protein levels in mouse models ().

What specific cell types can I detect using this antibody?

The Sca-1/Ly6 antibody is capable of detecting Sca-1/Ly6 antigen in mouse and rat cells, particularly in studies involving stem and progenitor cells, such as those derived from bone marrow-independent vascular wall-resident stem cells ().

Is this antibody suitable for immunohistochemistry applications?

Yes, the Sca-1/Ly6 antibody has been utilized in immunohistochemistry, as demonstrated in studies where it was used to label salivary gland tissues and other cell types to assess stem cell markers ().

How does this antibody perform in flow cytometry?

The Sca-1/Ly6 antibody has been cited in studies that employed flow cytometry to analyze stem cell populations, indicating its effectiveness in identifying and characterizing these cells ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Senolytic-sensitive p16Ink4a+ fibroblasts in the tumor stroma rewire lung cancer metabolism and plasticityCell Stem CellDecember 4, 2025Jin Young Lee, Nabora Reyes, Sang-Ho Woo, Nancy C. Allen, Tsukasa Kadota, Andrew Lechner, Ritusree Biswas, Sakshi Goel, Fia Stratton, Chaoyuan Kuang, et al.smooth muscle actin (1:200, Abcam, ab5694), PI16 (R&D systems, AF4929), LY6A (R&D systems, 2 μg/ml, AF1226), APOE (1:200, Invitrogen, 701241), E-Cadherin (1:200, Cell signaling, 3195S), Phospho-Histone H2A.X

(2) Mindin/spondin-2 regulates fibroblast subpopulations through distinct Src family kinases during fibrogenesisJCI InsightFebruary 10, 2025Sunny Kataria, Isha Rana, Krithika Badarinath, Rania F. Zaarour, Gaurav Kansagara, Sultan Ahmed, Abrar Rizvi, Dyuti Saha, Binita Dam, Abhik Dutta, et al.al., 2022, ref. 18; Pincha et al., 2018, ref. 49; Nakasaki et al., 2015, ref. 36); SCA1 (R&D Systems, AF1226); CD26 (R&D Systems, AF954); CD11b (Abcam, ab8878); CD3 (ebiosciences, 14-0032-85), and F4/80 (ebiosciences,

(3) Polyclonal regeneration of mouse bone marrow endothelial cells after irradiative conditioningCell reportsNovember 26, 2024Skulimowska I, Morys J, Sosniak J, Gonka M, Gulati G, Sinha R, Kowalski K, Mosiolek S, Weissman IL, Jozkowicz A, et al.,BD Biosciences,Cat# 558162; RRID: AB_647253 Ly6A/E (Sca-1) – unconjugated (polyclonal goat IgG),R&D Systems,Cat# AF1226; RRID: AB_354679 Donkey anti-Goat IgG (H + L) Cross-Adsorbed Secondary Antibody – Alexa Fluor 594 (polyclonal),Thermo Fisher Scientific

(4) Senescent fibroblasts in the tumor stroma rewire lung cancer metabolism and plasticitybioRxiv : the preprint server for biologyJuly 30, 2024Lee JY, Reyes N, Woo SH, Goel S, Stratton F, Kuang C, Mansfield AS, LaFave LM, Peng T.

(5) Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repairCell stem cellNovember 3, 2022Jeffery EC, Mann TLA, Pool JA, Zhao Z, Morrison SJ.

(6) CXCL12/CXCR4-Rac1-mediated migration of osteogenic precursor cells contributes to pathological new bone formation in ankylosing spondylitisScience advancesApril 8, 2022Cui H, Li Z, Chen S, Li X, Chen D, Wang J, Li Z, Hao W, Zhong F, Zhang K, et al.antibodies. Antibodies applied to mouse sections include sdf-1 (Abcam, ab9797), sca-1 (R&D Systems, AF1226), GFP (Abcam, ab13970), PRX-1 (Abcam, ab211292), CD45 (Abcam, ab10558), and F4/80 (Abcam, ab6640). For

(7) Bone marrow-independent adventitial macrophage progenitor cells contribute to angiogenesisCell death & diseaseMarch 9, 2022Kleefeldt F, Upcin B, Bömmel H, Schulz C, Eckner G, Allmanritter J, Bauer J, Braunger B, Rueckschloss U, Ergün S.number,Dilution CD31,Abcam,ab28364,1:200 CD34,Abcam,ab8158,1:50 F4/80,Abcam,ab111101,1:100 Ki67,Abcam,ab16667,1:50 Ly6c,Abcam,ab15627,1:100 Sca-1,R&D Systems,AF1226,1:100 VEGF,Abcam,ab46154,1:200 VEGFR2 (Flk-1),Santa Cruz,sc-393163,1:50 Antibodies used in this

(8) Fecal microbiota transplantation ameliorates atherosclerosis in mice with C1q/TNF-related protein 9 genetic deficiencyExperimental & molecular medicineFebruary 1, 2022Kim ES, Yoon BH, Lee SM, Choi M, Kim EH, Lee BW, Kim SY, Pack CG, Sung YH, Baek IJ, et al.CD19 (14-0194-82, Thermo, Waltham, MA, USA), CD68 (ab125212, Abcam, Cambridge, MA, USA), and Sca-1 (AF1226, R&D Systems, Minneapolis, MN, USA) primary antibodies. Anti-rabbit AF546, anti-mouse AF488, and anti-rat

(9) Distinct Expression Patterns of Cxcl12 in Mesenchymal Stem Cell Niches of Intact and Injured Rodent TeethInternational journal of molecular sciencesMarch 16, 2021Pagella P, Nombela-Arrieta C, Mitsiadis TA.A11122, Invitrogen/Thermo Fisher Scientific, Basel, Switzerland), goat pAb anti-Sca-1 (dilution 1:100; AF1226, R&D Systems, minneapolis, MI, USA), rabbit pAb anti-NG2 (dilution 1:100; AB5320, Millipore, Schaffhausen,

(10) Smart Hydrogels for the Augmentation of Bone Regeneration by Endogenous Mesenchymal Progenitor Cell RecruitmentAdvanced science (Weinheim, Baden-Wurttemberg, Germany)April 1, 2020Lienemann PS, Vallmajo-Martin Q, Papageorgiou P, Blache U, Metzger S, Kiveliö AS, Milleret V, Sala A, Hoehnel S, Roch A, et al.washing, the slides were soaked in rat serum for 1 h at RT and exposed to anti‐Sca‐1 antibody (R&D Systems AF1226) for at least 2 h at RT. The slides were incubated with a donkey anti‐goat FITC‐conjugated secondary

(11) Single-Cell Analysis of the Muscle Stem Cell Hierarchy Identifies Heterotypic Communication Signals Involved in Skeletal Muscle RegenerationCell reportsMarch 10, 2020De Micheli AJ, Laurilliard EJ, Heinke CL, Ravichandran H, Fraczek P, Soueid-Baumgarten S, De Vlaminck I, Elemento O, Cosgrove BD.AB_2077788 Anti-Mouse Laminin-α1,R&D Systems,Cat# MAB4656; RRID: AB_2265518 Anti-Mouse Sca-1/Ly6,R&D Systems,Cat# AF1226; RRID: AB_354679 Anti-Mouse C1q R1/CD93,R&D Systems,Cat# MAB1696; RRID: AB_2076062 Anti-Human/Mouse CX3CR1

(12) Tgfβ signaling is critical for maintenance of the tendon cell fateeLifeJanuary 21, 2020Tan GK, Pryce BA, Stabio A, Brigande JV, Wang C, Xia Z, Tufa SF, Keene DR, Schweitzer R.NBP1-06712SS RRID:AB_1625982 IF(1:100), Antigen retrieval Antibody Goat anti-Sca-1/Ly6 R and D Systems Cat# AF1226 RRID:AB_354679 IF(1:80) Antibody Rat anti-Sca-1/Ly6 R and D Systems Cat# MAB1226 RRID:AB_2243980 IF(1:50) Antibody Goat

(13) Genetic lineage tracing of targeted cell populations during enthesis healingJournal of orthopaedic research : official publication of the Orthopaedic Research SocietyDecember 1, 2018Moser HL, Doe AP, Meier K, Garnier S, Laudier D, Akiyama H, Zumstein MA, Galatz LM, Huang AH.using antibodies against Laminin (L9393, Sigma), α-smooth muscle actin (αSMA, C6198, Sigma) and Sca1 (AF1226, R&D Systems) with Cy3 secondary detection (711-166-152, Jackson Immuno Research) and DAPI counterstaining.

(14) Multicolor quantitative confocal imaging cytometryNature methodsJanuary 1, 2018Coutu DL, Kokkaliaris KD, Kunz L, Schroeder T.

(15) Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanismsScientific reportsAugust 25, 2017Golle L, Gerth HU, Beul K, Heitplatz B, Barth P, Fobker M, Pavenstädt H, Di Marco GS, Brand M.

(16) Dipeptidyl Peptidase-4 Regulates Hematopoietic Stem Cell Activation in Response to Chronic StressJournal of the American Heart AssociationJuly 14, 2017Zhu E, Hu L, Wu H, Piao L, Zhao G, Inoue A, Kim W, Yu C, Xu W, Bando YK, et al.staining. Double immunofluorescence was performed using a goat polyclonal antibody to sca‐1 (1:100; Cat. AF1226; R&D Systems, Dallas, TX) and a rabbit polyclonal antibody Ki67 antibody (1:50; #9129; Cell Signaling Technology

(17) Systemic application of sirolimus prevents neointima formation not via a direct anti-proliferative effect but via its anti-inflammatory propertiesInternational journal of cardiologyJuly 1, 2017Daniel JM, Dutzmann J, Brunsch H, Bauersachs J, Braun-Dullaeus R, Sedding DG.

(18) Epidermal β-catenin activation remodels the dermis via paracrine signalling to distinct fibroblast lineagesNature communicationsFebruary 3, 2016Lichtenberger BM, Mastrogiannaki M, Watt FM.immunofluorescence labelling: Lrig: R&D Systems, FAB3688G; CD26: R&D Systems, AF954; Sca1: R&D Systems, AF1226; PDGFRa: R&D Systems, AF1062; Collagen III: Abcam, ab7778, Collagen11a1: Abcam, ab64883; Elastin: Abcam,

(19) Pluripotent stem cells derived from mouse and human white mature adipocytesStem cells translational medicineFebruary 1, 2014Jumabay M, Abdmaulen R, Ly A, Cubberly MR, Shahmirian LJ, Heydarkhan-Hagvall S, Dumesic DA, Yao Y, Boström KI.

(20) Wnt5a suppresses tumor formation and redirects tumor phenotype in MMTV-Wnt1 tumorsPloS oneJanuary 1, 2014Easter SL, Mitchell EH, Baxley SE, Desmond R, Frost AR, Serra R.Cell Signaling Technology, Beverly, MA, USA, #9562), anti-Sca-1 (1∶1000, R&D Systems, Minneapolis, MN, USA, #AF1226), anti-Keratin 8 (1∶1000, Covance, Princeton, NJ, USA, #MMS-162P), anti-Keratin 6 (1∶1000, Covance,

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