TNF-alpha Antibody AF-410-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemTNF-alpha Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF-410-NA
NCBI Gene AliasesTnlg1f, TNFalpha, DIF, Tnfsf1a, TNF-a, TNFA, TNFSF2, TNF-alpha, TNLG1F, Tnfa
ApplicationsBioassay,ELISA Development,ELISA Development (Capture),Luminex Development,Assay Development,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Neutralization,ELISA Capture (Matched Antibody Pair),Intracellular Staining by Flow Cytometry,Immunocytochemistry,CyTOF-ready,Functional Assay,Cell Culture,ELISA Capture
IsotypeIgG
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Porcine,Rat,Transgenic Mouse
Quantity100 ug (also 25 ug)
TargetTNF-alpha
HostGoat
NCBI Full Gene Nametumor necrosis factor
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse TNF-alpha, Leu80-Leu235, Accession # P06804
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse TNF-alpha in ELISAs. Detects human and mouse TNF-alpha in Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat TNF-alpha is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study TNF-alpha in mouse models, particularly in relation to inflammation?

Yes, the TNF-alpha antibody has been shown to work effectively in mouse models. For instance, it was utilized in a study investigating the effects of TNF-alpha on adipocyte dedifferentiation and inflammation in adipose tissue, demonstrating its relevance in understanding inflammatory processes in obesity (Neugebauer et al., 2024).

Is this antibody suitable for use in immunohistochemistry applications?

The TNF-alpha antibody has been cited in various studies for use in immunohistochemistry. For example, it was employed to detect TNF-alpha production by macrophages in tissue samples, indicating its applicability in visualizing cytokine expression in histological sections (Picod et al., 2022).

What evidence is there for the effectiveness of this antibody in blocking TNF-alpha activity?

The TNF-alpha antibody has been used in experiments to neutralize TNF-alpha in vitro, which has been shown to decrease inflammatory responses in cell cultures. In one study, it was applied to block TNF-alpha in macrophage cultures, leading to reduced inflammation and cell death (Ramkhela et al., 2014).

Does this antibody demonstrate cross-reactivity with other species, and how might that affect its use in experiments?

The TNF-alpha antibody exhibits approximately 50% cross-reactivity with recombinant rat TNF-alpha, which may be beneficial for studies involving multiple species, such as porcine, human, mouse, and rat. This cross-reactivity allows for broader applications in comparative studies of TNF-alpha across different models (R&D Systems).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Divergence in poxvirus-encoded E3-like proteins can dictate poxvirus activation of cellular necroptosisJournal of VirologyJuly 21, 2026Junior A. Enow, Saige Munig, Mathew L. Sample, Gil Speyer, Raghavi C. Hundekar, Jacqueline Williams, James Bonner, Yize Li, Grant McFadden, Bertram Jacobs, et al.mouse MLKL (Cell Signaling #37705S), mouse RIPK3 (Cell Signaling #95702), mouse TNF-alpha (R&D Systems #AF-410-NA, Invitrogen #16-7321-85), J2 dsRNA (Nordic-MUbio #SCICONS J2 – 10010200), and Z-NA (Novus Biologicals

(2) A positive feedback loop between FGR and p65 sustains satellite glial cell activation and chronic neuropathic painCell reportsFebruary 24, 2026Huang Y, He Y, Wang Z, Zhang F, Han M, Guo X, Xie Y, Wang J, Ma X, Dan J, et al.

(3) N-phenylquinazolin-4-amine-based EGFR TKIs suppress pulmonary fibrosis by modulating the EGFR/ERBB3 axis in epithelial-macrophage interactionCommun BiolDecember 1, 2025Ji-Hee Kim, Seo-Hyun Choi, Jae-Kyung Nam, Min-Sik Park, Haeng Ran Seo, Inhee Choi, Hae-June Lee, Jaeho Cho, Jeeyong Lee, Yoon-Jin Lee#NBP1-32731), iNOS (IHC/IF 1:100; Santa Cruz Biotechnology; #sc7271), TNF-α (IHC/IF 1:100; R&D System; #AF-410NA), IL-6 (IHC/IF 1:200; Abcam; #ab208113), IFN-γ (IHC/IF 1:200; Abcam; #ab216642), EGF (IHC/IF 1:100;

(4) Targeting SHP2 to reverse immune evasion and resistance to anti-PD-1 therapy in non-small cell lung cancerCell Communication and SignalingNovember 6, 2025Shiwei Chen, Dingguo Wang, Dian Xiong, Lingxian Zhang, Kunchao Li, Jing Wang, Xiong Ma, Xingchen Hu, Lei Zeng, Jianjun Xu, et al.IFN-γ, [BioLegend, Cat# 517,901, Dilution 1:800]). Anti-TNF-α (Goat anti-Mouse TNF-α, [R&D Systems, Cat# AF-410-NA, Dilution 1:400]). Following primary antibody incubation, sections were washed and incubated with an

(5) 25-Hydroxycholesterol modulates microglial function and exacerbates Alzheimer's disease pathology: mechanistic insights and therapeutic potential of cholesterol esterification inhibitionJ NeuroinflammationFebruary 25, 2025Hayoung Choi, Haeng Jun Kim, Sang-Eun Lee, Hyun Ho Song, Jieun Kim, Jihui Han, June-Hyun Jeong, Do Yup Lee, Sunghoe Chang, Inhee Mook-JungGoat polyclonal anti-mouse Il-1β, R&D systems, AF-401-NA; Human/Mouse TNF-α antibody, R&D systems, AF-410-NA; Anti-PSD95 antibody, Abcam, ab18258; Anti-Synaptophysin antibody, Millipore Sigma,, MAB368; Anti-TMEM119

(6) KIRA6 is an Effective and Versatile Mast Cell Inhibitor of IgE-mediated ActivationEuropean journal of immunologyFebruary 1, 2025Wunderle V, Wilhelm T, Boukeileh S, Goßen J, Margreiter MA, Sakurov R, Capellmann S, Schwoerer M, Ahmed N, Bronneberg G, et al.coated with capturing anti‐IL‐6 (1:250, BD Biosciences, #554400) or anti‐TNF (1:200, R&D Systems, #AF410‐NA) antibodies diluted in PBS overnight at 4°C according to manufacturer's instructions. ELISA plates were

(7) The Impact of Resident Adipose Tissue Macrophages on Adipocyte Homeostasis and DedifferentiationInternational Journal of Molecular SciencesDecember 4, 2024Julia Neugebauer, Nora Raulien, Lilli Arndt, Dagmar Akkermann, Constance Hobusch, Andreas Lindhorst, Janine Fröba, Martin Gerickewas used as solvent control. For cytokine neutralization experiments, 1 or 10 ng/mL TNFα antibody (AF-410-NA, R&D Systems, biotechne, Minneapolis, MN, USA) dissolved in PBS was added to the explant culture media

(8) Distinct RORγt-dependent Th17 immune responses are required for autoimmune pathogenesis and protection against bacterial infectionCell reportsNovember 26, 2024Zhong X, Wu H, Zhang W, Shi Y, Gwack Y, Xue HH, Sun Z.anti-mouse IL-12,R&D Systems,Cat# AF-419-NA; RRID:AB_354485 Goat polyclonal anti-mouse TNF-α,R&D Systems,Cat# AF-410-NA; RRID:AB_354479 Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains Citrobacter rodentium

(9) Covalently grafted human serum albumin coating mitigates the foreign body response against silicone implants in miceBioactive materialsApril 1, 2024Zhou X, Hao H, Chen Y, Cao W, Zhu Z, Ni Y, Liu Z, Jia F, Wang Y, Ji J, et al.

(10) The IL-33/ST2 axis is protective against acute inflammation during the course of periodontitisNature communicationsMarch 28, 2024Liu A, Hayashi M, Ohsugi Y, Katagiri S, Akira S, Iwata T, Nakashima T.

(11) Mucosal SARS-CoV-2 vaccination of rodents elicits superior systemic T central memory function and cross-neutralising antibodies against variants of concernEBioMedicineJanuary 1, 2024O'Neill A, Mantri CK, Tan CW, Saron WAA, Nagaraj SK, Kala MP, Joy CM, Rathore APS, Tripathi S, Wang LF, et al.was done for IFN-γ (anti-IFN-γ-BV711, BD Biosciences 554412) and TNFα (goat-anti-TNFα, R&D Systems AF-410-NA, anti-goat-IgG-FITC, Jackson ImmunoResearch, 305-096-006) for 1 h in permeabilisation solution. Data

(12) Paper-Based Coculture Platform to Evaluate the Effects of Fibroblasts on Estrogen Signaling in ER+ Breast CancersACS Measurement Science AuDecember 20, 2023Zachary R. Sitte, Abel Andre Miranda Buzetta, Sarina J. Jones, Zhi-Wei Lin, Nathan Ashbrook Whitman, Matthew R. LockettAssay, and Reporter Lysis 5× buffer were purchased from Promega. Anti-IL-6 (MAB206) and anti-TNF-α (AF410NA) neutralizing antibodies were purchased from R&D Systems. Cell Culture and Maintenance Cell culture medium

(13) Soluble epoxide hydrolase-targeting PROTAC activates AMPK and inhibits endoplasmic reticulum stressBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapieDecember 1, 2023Peyman M, Barroso E, Turcu AL, Estrany F Jr, Smith D, Jurado-Aguilar J, Rada P, Morisseau C, Hammock BD, Valverde ÁM, et al.Santa Cruz Biotechnology Inc.), phosphorylated STAT3 Tyr705 (#9131, Cell Signaling Technology), TNF-α (AF410-NA, R&D Systems, Minneapolis, MN, USA), TRB3 (sc-365842, Santa Cruz Biotechnology Inc.), vinculin (sc-25336,

(14) Intercellular mitochondrial transfer alleviates pyroptosis in dental pulp damageCell proliferationSeptember 1, 2023Wang K, Zhou L, Mao H, Liu J, Chen Z, Zhang L.

(15) Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNFαCell & bioscienceJune 24, 2023Oppezzo A, Monney L, Kilian H, Slimani L, Maczkowiak-Chartois F, Rosselli F.(Sigma, #P4359), butyrolactone I (Sigma, #203988) and human/mouse anti-TNF-alpha antibody (R&D Systems, #AF-410-NA) at the indicated concentrations. Antisense oligonucleotides directed against p21 were purchased from

(16) Transformation of primary murine peritoneal mast cells by constitutive KIT activation is accompanied by loss of Cdkn2a/Arf expressionFrontiers in immunologyJanuary 1, 2023Capellmann S, Sonntag R, Schüler H, Meurer SK, Gan L, Kauffmann M, Horn K, Königs-Werner H, Weiskirchen R, Liedtke C, et al.

(17) Deregulated JAK3 mediates growth advantage and hemophagocytosis in extranodal nasal-type natural killer/T-cell lymphomaHaematologicaSeptember 1, 2022Picod A, Martino S, Cervera P, Manuceau G, Arca M, Wittner M, Zhang Y, Liang H, Beghi F, Solary E, et al.France) shows interferon-γ production by malignant cells. (D) Tumor necrosis factor-a (TNF-a) stain (AF410-NA, R&D System, Minneapolis, MN, USA) shows TNF-a production by macrophages, identified as large irregularly

(18) Murine macrophages or their secretome delivered in alginate dressings enhance impaired wound healing in diabetic miceBiomaterialsSeptember 1, 2022Theocharidis G, Rahmani S, Lee S, Li Z, Lobao A, Kounas K, Katopodi XL, Wang P, Moon S, Vlachos IS, et al.

(19) Effect of rottlerin on astrocyte phenotype polarization after trimethyltin insult in the dentate gyrus of miceJournal of neuroinflammationJune 11, 2022Hwang Y, Kim HC, Shin EJ.with primary antibody against interleukin-1β (IL-1β, 1:1,000; #MAB401, R&D Systems), TNFα (1:500; # AF-410-NA, R&D Systems), or β-actin (1:300,000; #A5441, Sigma-Aldrich). Membranes were then incubated with horseradish

(20) Molecular mechanism of the TGF‑β/Smad7 signaling pathway in ulcerative colitisMolecular medicine reportsApril 1, 2022Bai B, Li H, Han L, Mei Y, Hu C, Mei Q, Xu J, Liu X.Institute supplied kits for the detection of myeloperoxidase (MPO; cat. no. A044-1-1). Anti-TNF-α (cat. no. AF-410-NA) was obtained from R&D Systems, Inc. The MLCK ELISA kit (cat. no. ELA06754) was purchased from RapidBio.

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Human/Mouse TNF-alpha Antibody