Galectin-9 Antibody AF2045 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGalectin-9 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF2045
ApplicationsBinding Assay,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry,Cell Culture
Quantity100 ug (also 25 ug)
TargetGalectin-9
HostGoat
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
ReactivityHuman,Mouse
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human Galectin-9
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namegalectin 9
NCBI Gene AliasesHUAT, LGALS9A

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Galectin-9 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Galectin-3 and rhGalectin-4 is observed and less than 2% cross-reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-7, and rhGalectin-8 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Altered distribution of tissue galectins correlates with mucosal dysregulation in SIV infectionThe Journal of ImmunologyJanuary 21, 2026Thomas A Premeaux, Stephen T Yeung, Samuel D Johnson, Preeti Moar, Siddappa N Byrareddy, Lishomwa C Ndhlovublocking buffer (1:300) for 1 h. Primary antibodies include those targeting human galectin-9 (Cat. AF2045; R&D Systems) and galectin-3 (Cat. AF1197; R&D Systems), galectin-1 (Cat. Ab108389; Abcam), and ZO-1

(2) Proteomic Analysis of Small Extracellular Vesicles From Lymphatic Affluents in Developing Premetastatic Niche in MelanomaMolecular & Cellular ProteomicsJanuary 1, 2026Shankar Suman, Liyi Geng, Wendy K. Nevala, Raymond Moore, Chathu Atherton, Xiaowei Zhao, Jaeyun Sung, Ray Guo, James W. Jakub, Richard K. Kandasamy, et al.albumin (catalog no.: 4929; Cell Signaling Technology), calnexin (ab22595; abcam), galectin-9 (LGALS9, AF2045; R&D Systems), CD3D (ab229280; abcam), and CD38 (ab226034; abcam) in blocking buffer under constant

(3) Galectin-9 binding to HLA-DR in dendritic cells controls immune synapse formation and T cell proliferationProceedings of the National Academy of Sciences of the United States of AmericaDecember 16, 2025Rodgers-Furones A, Brands T, van Gameren G, Florencio-Zabaleta M, Bathini M, Delgado S, Wijfjes Z, Fedorova K, Classens R, Kroese L, et al.ice to block nonspecific interaction prior to being stained with a goat anti-galectin-9 antibody (#AF2045, R&D systems) at 8 μg/ml for 30 min at 4 °C. A donkey-anti-goat secondary antibody conjugated to Alexa

(4) Galectin-9 regulates dendritic cell polarity and uropod contraction by modulating RhoA activityJournal of Cell BiologyNovember 3, 2025Guus A. Franken, Harry Warner, Jorge Cuenca-Escalona, Isabel F. Stehle, Vince P.A. van Reijmersdal, Sophie E. Klomp, Koert Schreurs, Andrea Rodgers-Furones, Rohit Rajesh Gokhale, Manon Vullings, et al.and PGE2 (10 µg/ml; Pfizer). When necessary, moDCs were treated with recombinant galectin-9 protein (AF2045; R&D Systems) at a final concentration of 1 µg/ml. Isolation and culture of primary cells Human cDC2s

(5) Autophagy-independent role of ATG9A vesicles as carriers for galectin-9 secretionNat CommunMay 7, 2025Wenting Zhang, Cuicui Ji, Xianghua Li, Tianlong He, Wei Jiang, Yukun Liu, Meiling Wu, Yunpeng Zhao, Xuechai Chen, Xiaoli Wang, et al.(Thermo Fisher Scientific, PA5-115266, 1:1000), rabbit anti-galectin-9 (abcam, ab227046, 1:1000), goat anti-galectin-9 (AF2045, R&D systems, 1:500), mouse anti-GAPDH (proteintech, 60004-1-Ig, 1:10000), rabbit anti-ATG2A (MBL, PD041,

(6) Galectin-9 as a new biomarker of acute-on-chronic liver failureScientific reportsSeptember 27, 2024Ling J, You S, Chen W, Yang X, Xv Y, Zhu B.S1). Immunofluorescence staining Immunofluorescence staining was performed using Gal-9-specific antibodies (AF2045, R&D Systems; 1:400) and CD68 antibodies (MAB20401, R&D Systems; 1:2000) on slides. The Supplementary

(7) Increased Galectin-9 Levels Correlate with Disease Activity in Patients with DMARD-Naïve Rheumatoid Arthritis and Modulate the Secretion of MCP-1 and IL-6 from Synovial FibroblastsCellsJanuary 15, 2023Nielsen MA, Køster D, Mehta AY, Stengaard-Pedersen K, Busson P, Junker P, Hørslev-Petersen K, Hetland ML, Østergaard M, Hvid M, et al.5 μg/mL rhGal-9 (9064-GA, R&D Systems). The bound Gal-9 was detected using goat anti-Gal-9 5 μg/mL (AF2045, R&D Systems), followed by fluorescent anti-goat IgG-633 (H + L) 5 μg/mL (A21086, Thermo Fischer). In

(8) Intracellular Galectin-9 Controls Dendritic Cell Function by Maintaining Plasma Membrane RigiditySSRN Electronic JournalMarch 31, 2022Laia Querol Cano et al.rabbit anti-DC-SIGN (H200, Santa Cruz, Heidelberg, Germany) at 1:2000 (v/v), goat anti-Galectin-9 (AF2045, R&D systems, Minneapolis, Minnesota) at 1:1000 (v/v) and rat anti-tubulin (Novus Biological, Abingdon,

(9) Bacterial lectin BambL acts as a B cell superantigenCellular and molecular life sciences : CMLSDecember 1, 2021Frensch M, Jäger C, Müller PF, Tadić A, Wilhelm I, Wehrum S, Diedrich B, Fischer B, Meléndez AV, Dengjel J, et al.polyclonal #2712; SYK phospho-Tyr525/526: clone C87C1 #2710), Bio-techne (galectin-9: polyclonal, #AF2045), Sigma-Aldrich (α-tubulin: clone B-5–1-2, #T5168), or Eurogentec (BambL: polyclonal, custom Ab against

(10) Aminophylline modulates the permeability of endothelial cells via the Slit2-Robo4 pathway in lipopolysaccharide-induced inflammationExperimental and therapeutic medicineSeptember 1, 2021Chen Q, Zhou X, Hou R, Zhou Z, Wang Z, Chen Y, Weng J, Xu J.no. MAB50041), VE-cadherin (cat. no. AF1002), fibronectin (cat. no. AF1918) and integrin (cat. no. AF2045) primary antibodies were purchased from R&D Systems, Inc. Alexa Fluor 488- (cat. no. sc-362257) and

(11) High Expression of TIM 3 and Galectin 9 on Immunohistochemistry Staining of Tumor Specimen at Diagnosis in Pediatric Patients with Ewing SarcomaJournal of cancer immunologyJanuary 1, 2021Si SJ, Wertheim GB, Barrett DM.Marque,156-R-95,1:400,E2-20,Leica Bond Refine ALK,D5F3,Cell Signaling,3633,1:200,P.C. Citrate,IF Galectin 9,n/a,R&D Systems,AF2045,1:50,P.C. Citrate,IF MHCII HLA-DR,TAL.1B5,Dako,M0746,1:100,E1-20,Leica Bond Refine Summary of antibodies

(12) Lumenal Galectin-9-Lamp2 interaction regulates lysosome and autophagy to prevent pathogenesis in the intestine and pancreasNature communicationsAugust 27, 2020Sudhakar JN, Lu HH, Chiang HY, Suen CS, Hwang MJ, Wu SY, Shen CN, Chang YM, Li FA, Liu FT, et al.rabbit anti-β4galt1 (MyBiosource #MBS8242969) (IB: 1:1000), goat anti-human galectin-9 (R&D Systems #AF2045) (IB: 1:1000), rabbit anti-human galectin-9 (Sigma #HPA046876) (IHC: 1:100), rabbit anti-M6PR (cation independent

(13) Aspergillus fumigatus enhances human NK cell activity by regulating M1 macrophage polarizationMolecular medicine reportsAugust 1, 2019Zhang X, He D, Gao S, Wei Y, Wang L.NK cells from the same individual for a further 12 h, in the presence or absence of Galectin-9 (cat. no. AF2045; R&D Systems, Inc.)-, IL-18 (cat. no. D044-3; R&D Systems, Inc.)- or TNF-α (cat. no. AF-410-NA; R&D Systems, Inc

(14) Circulating levels of PD-L1 and Galectin-9 are associated with patient survival in surgically treated Hepatocellular Carcinoma independent of their intra-tumoral expression levelsScientific reportsJuly 23, 2019Sideras K, de Man RA, Harrington SM, Polak WG, Zhou G, Schutz HM, Pedroza-Gonzalez A, Biermann K, Mancham S, Hansen BE, et al.

(15) Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of AllergenJournal of immunology (Baltimore, Md. : 1950)August 1, 2017Schroeder JT, Bieneman AP.

(16) Tim-3 Is Upregulated in NK Cells during Early Pregnancy and Inhibits NK Cytotoxicity toward Trophoblast in Galectin-9 Dependent PathwayPloS oneJanuary 1, 2016Sun J, Yang M, Ban Y, Gao W, Song B, Wang Y, Zhang Y, Shao Q, Kong B, Qu X.

(17) Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cellsPloS oneJanuary 1, 2015Leskela S, Serrano A, de la Fuente H, Rodríguez-Muñoz A, Ramos-Levi A, Sampedro-Nuñez M, Sánchez-Madrid F, González-Amaro R, Marazuela M.

(18) Galectin-1, -3 and -9 Expression and Clinical Significance in Squamous Cervical CancerPloS oneJanuary 1, 2015Punt S, Thijssen VL, Vrolijk J, de Kroon CD, Gorter A, Jordanova ES.M3/38, RRID: AB 1134237, lot B160878, BioLegend, London, UK) and polyclonal goat anti-galectin-9 (1:200, AF2045, RRID: AB 2137232, lot KNI0112011, R&D Systems, Abingdon, UK) diluted in 1% w/v bovine serum albumin

(19) Human cytomegalovirus upregulates expression of the lectin galectin 9 via induction of beta interferonJournal of virologySeptember 1, 2014McSharry BP, Forbes SK, Cao JZ, Avdic S, Machala EA, Gottlieb DJ, Abendroth A, Slobedman B.

(20) Galectin expression profiling identifies galectin-1 and Galectin-9Δ5 as prognostic factors in stage I/II non-small cell lung cancerPloS oneJanuary 1, 2014Schulkens IA, Heusschen R, van den Boogaart V, van Suylen RJ, Dingemans AM, Griffioen AW, Thijssen VL.

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Galectin-9 Antibody

Galectin-9 Antibody

Galectin-9 Antibody

Galectin-9 Antibody

Galectin-9 Antibody

Galectin-9 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (27)

Reviews

Galectin-9 Antibody from R&D Systems, a Bio-Techne Brand

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