DPPIV/CD26 Antibody AF1180 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemDPPIV/CD26 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF1180
ApplicationsBlocking,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Neutralization,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry
IsotypeIgG
TargetDPPIV/CD26
HostGoat
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityBat,Camelid,Ferret,Human,Mouse,Primate,Rat
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human DPPIV/CD26 (Catalog # 1180-SE ), Asp34-Pro766, Accession # Q53TN1
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namedipeptidyl peptidase 4
NCBI Gene AliasesADABP, ADCP2, CD26, DPPIV, TP103

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human DPPIV/CD26 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse DPPIV is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study DPP4 in organoid models, particularly in relation to coronavirus research?

Yes, the DPPIV/CD26 antibody has been utilized in studies involving organoid models to investigate host factors for coronaviruses. For instance, it was used to verify DPP4 as an entry receptor for MERS-CoV in intestinal organoids, highlighting its relevance in viral research ().

Is there evidence that this antibody can be effectively used in cancer research, specifically regarding malignant mesothelioma?

The DPPIV/CD26 antibody has been referenced in studies examining its role in malignant mesothelioma, where it was used to assess the expression of CD26 in tumor tissues. This research suggests that CD26 is involved in tumor growth and invasion, indicating the antibody's utility in cancer studies ().

How has this antibody been applied in the context of flow cytometry?

The DPPIV/CD26 antibody has been employed in flow cytometry to analyze DPP4 expression in transduced cells. This application demonstrates its effectiveness in quantifying protein expression levels in a cellular context ().

What can you tell me about the specificity of this antibody in detecting DPP4 across different species?

The DPPIV/CD26 antibody is reactive with multiple species, including human, mouse, rat, and non-human primates, making it versatile for comparative studies across different mammalian models ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) PET Imaging of Cardiac Inflammation in Viral Myocarditis Using a DPP4‐Targeted ProbeAdvanced ScienceApril 15, 2026Wanhao Gao, Wenzhu Hu, Du Tang, Xinxin Liu, Kun Yu, Lixia Feng, Minyu Liao, Wang Lin, Wenqiang Zhu, Sanjay Rajagopalan, et al.included the following: mouse DPP4/CD26 antibody (AF954, R&D Systems, USA), human DPP4/CD26 antibody (AF1180, R&D systems, USA), GAPDH monoclonal antibody (60004‐1, Proteintech, USA), HRP‐conjugated rabbit anti‐goat

(2) Human receptive endometrial assembloid for deciphering the implantation windoweLifeApril 1, 2026Yu Zhang, Rusong Zhao, Chaoyan Yang, Jinzhu Song, Peishu Liu, Yan Li, Boyang Liu, Tao Li, Changjian Yin, Minghui Lu, et al.RRID:AB_10609510,IF (1:50) Antibody,Goat anti-Human DPPIV/CD26 (Goat polyclonal),R&D Systems,Cat#: AF1180 RRID:AB_354651,IF (1:100) Antibody,HIF-1 alpha antibody (Mouse monoclonal),NOVUS,Cat#: NB 100–105 RRID:AB_350048,IF

(3) Human receptive endometrial assembloid for deciphering the implantation windowElifeApril 1, 2026Yu Zhang, Rusong Zhao, Chaoyan Yang, Jinzhu Song, Peishu Liu, Yan Li, Boyang Liu, Tao Li, Changjian Yin, Minghui Lu, et al.RRID:AB_10609510,IF (1:50) Antibody,Goat anti-Human DPPIV/CD26 (Goat polyclonal),R&D Systems,Cat#: AF1180 RRID:AB_354651,IF (1:100) Antibody,HIF-1 alpha antibody (Mouse monoclonal),NOVUS,Cat#: NB 100–105 RRID:AB_350048,IF

(4) Human keratinocytes exhibit limited potential for SARS-CoV-2 infection despite ACE2 and mature cathepsin L expressionJID InnovationsMarch 1, 2026Leslie Hertereau, Manon Barthe, Noura Lamghari, Peggy Merida, Gaelle Pommier, Elisabeth Pinel, Jitendriya Swain, Delphine Muriaux, Hanan Osman-Ponchet, Véronique M. BraudThermo Fisher Scientific); anti-AXL, polyclonal goat IgG AF154; and anti-DPP4, polyclonal goat IgG AF1180 (R&D Systems, Bio-Techne SAS, Noyal Châtillon, France). Antibody binding was detected using SuperSignal

(5) PDGFRα+DPP4+ Fibroblasts‐Macrophage Crosstalk Induces Orbital Fibrosis in Treatment‐Resistant Thyroid Eye Disease via the GAS6‐AXL PathwayAdvanced ScienceSeptember 29, 2025Lu Cheng, Jinwei Cheng, Guiling Liang, Xiaorui Wang, Yanwen Ge, Jiapei Liu, Luwei Cai, Hui Ying, Fenfen Wang, Jing Hu, et al.incubation with primary antibodies, including DPP4 (R&D Systems, Minneapolis, MN, USA, catalogue no. AF1180, 1:250), CD68 (Invitrogen, Waltham, MA, USA, catalogue no. PA5‐89134, 1:100), CD206 (Abcam, China, catalogue

(6) Identification of a Growth-Promoting Gene Cluster in the Region 2q24 as a Driver of Tumorigenesis in Childhood HepatoblastomaThe American Journal of PathologyAugust 1, 2025Martin M. Rodemann, Verena Dreschmann, Evelyn Dörner, Anette Sommer, Joern Kraetzschmar, Ludger Klein-Hitpass, Genta Nagae, Eiso Hiyama, Dietrich von Schweinitz, Roland Kappler, et al.NBP1-90056; RRID: AB_11044732; Novus Biologicals, Centennial, CO), DPP4 (goat polyclonal antibody, number AF1180; RRID: AB_354651; R&D Systems, Minneapolis, MN), FIGN (rabbit polyclonal antibody, number LS-C498842;

(7) Altered chaperone-nonmuscle myosin II interactions drive pathogenicity of the UNC45A c.710T>C variant in osteo-oto-hepato-enteric syndromeJCI InsightMarch 24, 2025Stephanie Waich, Karin Kreidl, Julia Vodopiutz, Arzu Meltem Demir, Adam R. Pollio, Vojtěch Dostál, Kristian Pfaller, Marianna Parlato, Nadine Cerf-Bensussan, Rüdiger Adam, et al.MilliporeSigma), rabbit anti-Rab11a (1:100; from Invitrogen, 715300), and goat anti-DPPIV/CD26 (1:10; AF1180 from R&D Systems, Bio-Techne), along with appropriate secondary antibodies (British Biocell International,

(8) The neonatal Fc receptor is a cellular receptor for human astrovirusNature MicrobiologyNovember 22, 2024Harshad Ingle, Jerome M. Molleston, Paige D. Hall, Duyen Bui, Leran Wang, Karan D. Bhatt, Lynne Foster, Avan Antia, Siyuan Ding, Sanghyun Lee, et al.10μg/ml of goat IgG isotype control (Invitrogen, #02–6202) or human DPP4/CD26 antibody (R&D Systems; AF1180) 12h prior to HAstV infection. The treatments were maintained throughout the experiment. For FcRn blocking

(9) The GTPase RAB6 is required for stem cell maintenance and cell migration in the gut epitheliumDevelopmentNovember 1, 2024Pierre Simonin, Gehenna Lobo Guerrero, Sabine Bardin, Ram Venkata Gannavarapu, Denis Krndija, Joseph Boyd, Stephanie Miserey, Danijela Matic Vignjevic, Bruno Goudantibodies were used: mouse anti-CD29 (1:100, BD Biosciences, 610467), goat anti-DPPIV (1:100, R&D Systems, AF1180), goat anti-collagen type IV (1:2000, Sigma-Aldrich, AB769), rat anti-ZO-1 (1:100, Sigma-Aldrich, MABT339),

(10) Resistance to SARS-CoV-2 infection in camelid nasal organoids is associated with lack of ACE2 expressionnpj VirusesSeptember 2, 2024Tim I. Breugem, Samra Riesebosch, Debby Schipper, Anna Z. Mykytyn, Petra van den Doel, Joaquim Segalés, Mart M. Lamers, Bart L. HaagmansDPP4 were stained using goat-anti-hACE2 (R&D Systems; AF933; 1:100) or goat-anti-hDPP4 (R&D Systems; AF1180; 1:100) and visualized with rabbit-anti-goat (Dako; P0160; 1:200) horseradish peroxidase labelled secondary

(11) Transmission dynamics of MERS-CoV in a transgenic human DPP4 mouse modelnpj VirusesAugust 21, 2024Neeltje van Doremalen, Trenton Bushmaker, Robert J. Fischer, Atsushi Okumura, Dania M. Figueroa Acosta, Rebekah J. McMinn, Michael Letko, Dana Scott, Greg Saturday, Vincent J. Munster(Miltenyi Biotec). Cells were washed two times in PBS containing 2% FBS, and incubated with anti-DPP4 (AF1180, R&D systems, 8 µg/mL in PBS with 2% FBS). After 1 h at 4 °C, cells were washed as above and incubated

(12) The neonatal Fc receptor and DPP4 are human astrovirus receptorsbioRxiv : the preprint server for biologyJuly 12, 2024Ingle H, Molleston JM, Hall PD, Bui D, Wang L, Foster L, Antia A, Ding S, Lee S, Fremont DH, et al.

(13) Distinguishing two distinct types of salivary extracellular vesicles: a potential tool for understanding their pathophysiological rolesFrontiers in molecular biosciencesJanuary 1, 2024Ogawa Y, Miura Y, Ikemoto M, Ohnishi A, Goto Y, Aoki K, Motokurumada Y, Akimoto Y, Endo T, Tsujimoto M, et al.NBP2-29408; Novus Biologicals, Littleton, CO, USA), goat anti-DPP IV (polyclonal antibody, 1:1,000; AF1180, R&D Systems, Inc., Minneapolis, MN, US), rabbit anti-CD9 (1:1,000, EXOAB-CD9A-1; System Biosciences,

(14) Adolescents with obesity treated with exenatide maintain endogenous GLP-1, reduce DPP-4, and improve glycemic controlFront Endocrinol (Lausanne)November 1, 2023Rasmus Stenlid, Sara Y. Cerenius, Quan Wen, Banu Küçükemre Aydin, Hannes Manell, Azazul Chowdhury, Hjalti Kristinsson, Iris Ciba, Erik S. Gjessing, Katharina Mörwald, et al.ELISAs. Circulating DPP-4 was analyzed with a sandwich ELISA (R&D systems, Minneapolis, MN, Catalog # AF1180, RRID : AB_354651). Circulating plasma DPP-4 levels are shown to have a strong correlation with plasma

(15) A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entryCellFebruary 16, 2023Chen J, Yang X, Si H, Gong Q, Que T, Li J, Li Y, Wu C, Zhang W, Chen Y, et al.HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L),Proteintech,Cat#:SA00001-1; RRID:AB_2722565 Human DPPIV/CD26 Antibody,R&D systems,Cat#: AF1180; RRID:AB_354651 Donkey Anti-Goat IgG H&L (Alexa Fluor 488),Abcam,Cat#: ab150129; RRID:AB_2687506 Cy3

(16) A CRISPR screen in intestinal epithelial cells identifies novel factors for polarity and apical transporteLifeJanuary 20, 2023Klee KMC, Hess MW, Lohmüller M, Herzog S, Pfaller K, Müller T, Vogel GF, Huber LA.Sigma-Aldrich), DNP (EM 1:6-1:30, #71-3500, Invitrogen), DPP4 (IF/FACS 1:100, #HBB3/775/42, DSHB; EM 1:10, #AF1180, R&D Systems), E-cadherin (IF 1:200, EM 1:30, #610181, BD Bioscences), GALNT2 (WB 1:1000, #AF7507, Novus Biologicals

(17) Mapping prohormone processing by proteases in human enteroendocrine cells using genetically engineered organoid modelsProceedings of the National Academy of Sciences of the United States of AmericaNovember 16, 2022Beumer J, Bauzá-Martinez J, Veth TS, Geurts V, Boot C, Gilliam-Vigh H, Poulsen SS, Knop FK, Wu W, Clevers H.following antibodies: mouse anti-CPB1 (1:100; R&D systems, MAB2897), goat anti-DPP4 (1:200; R&D systems, AF1180), mouse anti-GLP1 (1:100; Santa Cruz, sc-73508), rabbit anti-proglucagon (1;100; Cell signaling, CST8233),

(18) Identification of potent inhibitors of SARS-CoV-2 infection by combined pharmacological evaluation and cellular network prioritizationiScienceSeptember 16, 2022Patten JJ, Keiser PT, Morselli-Gysi D, Menichetti G, Mori H, Donahue CJ, Gan X, Valle ID, Geoghegan-Barek K, Anantpadma M, et al.puromycin selection. For FACS, 5 × 106 transduced cells were stained with 1.25μg DPP4 antibody (R&D Systems AF1180) for 1 h at room temperature. After washing cells 2X with PBS, Chicken anti-goat-FITC secondary antibody

(19) Seminal-Plasma-Mediated Effects on Sperm Performance in HumansCellsJuly 8, 2022Turunen T, Magris M, Malinen M, Kekäläinen J.overnight with primary antibodies for ANP (1:2000, ab108310, Abcam, Amsterdam, Netherlands), DPP4 (1:1000, AF1180, R&D systems, Minneapolis, MN, USA), and NEP (1:1000, AF1182, R&D systems). Then, blots were incubated

(20) Dipeptidyl peptidase 4 expression is not associated with an activated fibroblast phenotype in idiopathic pulmonary fibrosisFrontiers in pharmacologyJanuary 1, 2022Kadefors M, Berlin F, Wildt M, Dellgren G, Rolandsson Enes S, Aspberg A, Westergren-Thorsson G.antibodies against PECAM1/CD31 (1:200, clone JC70A, Dako, M0823), DPP4/CD26 (1:200, polyclonal, R&D Systems, AF1180) and THY1/CD90 (1:200, clone EPR3132, Abcam, ab92574) or against DPP4/CD26 and pan-cytokeratin (1:100,

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