Western blot was performed using Anti-ASF1A Polyclonal Antibody (Product # PA5-75958) and a 22 kDa band corresponding to Histone chaperone ASF1A was observed across all the cell lines tested. Nuclear enriched extracts (30 µg lysate) of LNCaP (Lane 1), Hep G2 (Lane 2), Jurkat (Lane 3), MCF7 (Lane 4), A549 (Lane 5), HCT 116 (Lane 6), HT-29 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, (1:4000 dilution)) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Western blot was performed using Anti-ASF1A Polyclonal Antibody (Product # PA5-75958) and a 22 kDa band corresponding to Histone chaperone ASF1A was observed across all the cell lines tested. Nuclear enriched extracts (30 µg lysate) of LNCaP (Lane 1), Hep G2 (Lane 2), Jurkat (Lane 3), MCF7 (Lane 4), A549 (Lane 5), HCT 116 (Lane 6), HT-29 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, (1:4000 dilution)) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supplier Page from Thermo Fisher Scientific for ASF1A Antibody