IL-6 Antibody AF-406-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIL-6 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF-406-NA
NCBI Gene AliasesCDF, HSF, BSF-2, Il-6, IL-6, IFNB2, HGF, BSF2, IFN-beta-2
ApplicationsELISA Development (Capture),Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry
TargetIL-6
HostGoat
IsotypeIgG
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Rabbit,Rat
Conjugate/TagUnconjugated
Quantity100 ug (also 25 ug)
NCBI Full Gene Nameinterleukin 6
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse IL-6, Phe25-Thr211, Accession # P08505
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse IL-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat IL-6 and recombinant cotton rat IL-6 is observed and less than 15% cross-reactivity with recombinant human IL-6 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for immunofluorescence staining in my experiments?

Yes, the IL-6 antibody has been successfully used for immunofluorescence staining, as demonstrated in studies where it was applied at a dilution of 1:200 to visualize IL-6 expression in various tissues (, ).

What species does this antibody react with, and how specific is it?

The IL-6 antibody shows reactivity with mouse, human, and rabbit species. It has approximately 100% cross-reactivity with recombinant rat IL-6 and recombinant cotton rat IL-6, while showing less than 15% cross-reactivity with recombinant human IL-6, indicating a high specificity for mouse IL-6 ().

Has this antibody been cited in studies related to inflammatory diseases?

Yes, the IL-6 antibody has been cited in multiple studies investigating its role in inflammatory processes, including research on neuroinflammation and atherosclerosis, where IL-6 signaling was implicated in disease mechanisms (, ).

What is the recommended storage condition for this antibody after reconstitution?

After reconstitution, the IL-6 antibody should be stored under sterile conditions at -20 to -70 °C for up to six months to maintain its stability and activity ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Phosphoglycerate dehydrogenase interacts with and inhibits the protein kinase TAK1 to mitigate septic shockThe Journal of biological chemistryOctober 1, 2025Hu P, Ji Z, Xiong H, Yu S, Shan X, Dong H, Jing W, Wang J, Wang Z, Cui Y, et al.specific for F4/80 (1:200; Cat# ab16911, Abcam), IL-1β (1:200; Cat# 9722, Abcam), and IL-6 (1:200; Cat# AF-406-NA, R&D Systems) for immunofluorescence staining on 5-μm–thick frozen liver sections. Alexa Fluor 488–

(2) Effect of spermidine intake on overload-induced skeletal muscle hypertrophy in male micePhysiological reportsFebruary 1, 2025Iwata T, Shirai T, Tanimura R, Iwai R, Takemasa T.abcam), anti‐PGC‐1α (AB3242; Millipore), anti‐TNF‐α (TN3‐19, 12) (sc‐12,744; Santa Cruz), and anti‐IL‐6 (AF406NA; R&D Systems). 2.4 Immunohistochemistry and CSA analysis The plantaris muscles were covered with an

(3) Effect of spermidine intake on skeletal muscle regeneration after chemical injury in male micePhysiological reportsOctober 1, 2024Iwata T, Shirai T, Uemichi K, Tanimura R, Takemasa T.abcam), anti‐PGC‐1α (516,557; Millipore), anti‐TNF‐α (TN3‐19, 12) (sc‐12,744; Santa Cruz), anti‐IL‐6 (AF406NA; R&D Systems), and anti‐IL‐1β (H153, sc‐7884; Santa Cruz). 2.4 Immunohistochemistry and cross‐sectional

(4) Matrix stiffness regulates the triad communication of adipocytes/macrophages/endothelial cells through CXCL13Journal of Lipid ResearchSeptember 1, 2024Arthur Choisez, Seiichiro Ishihara, Takuro Ishii, Yidan Xu, Sepideh D. Firouzjah, Hisashi Haga, Ryoichi Nagatomi, Joji Kusuyama(AF-585-NA, R&D systems), mouse IL-1beta Antibody (AF-401-NA, R&D systems), and mouse IL-6 antibody (AF-406-NA, R&D systems). Oil red O staining Lipid droplet appearance was determined by oil red O staining as

(5) Sclerostin antibody corrects periodontal disease in type 2 diabetic miceJCI insightJuly 18, 2024Turkkahraman H, Flanagan S, Zhu T, Akel N, Marino S, Ortega-Gonzalez D, Yuan X, Bellido T.

(6) Covalently grafted human serum albumin coating mitigates the foreign body response against silicone implants in miceBioactive materialsApril 1, 2024Zhou X, Hao H, Chen Y, Cao W, Zhu Z, Ni Y, Liu Z, Jia F, Wang Y, Ji J, et al.

(7) The IL-33/ST2 axis is protective against acute inflammation during the course of periodontitisNature communicationsMarch 28, 2024Liu A, Hayashi M, Ohsugi Y, Katagiri S, Akira S, Iwata T, Nakashima T.

(8) The super-healing MRL strain promotes muscle growth in muscular dystrophy through a regenerative extracellular matrixJCI insightJanuary 4, 2024O'Brien JG, Willis AB, Long AM, Kwon J, Lee G, Li FW, Page PG, Vo AH, Hadhazy M, Spencer MJ, et al.MilliporeSigma), anti–TGF-β1 (catalog 218981AP, Proteintech and catalog MA515065, Invitrogen), anti–mIL-6 (catalog AF-406-NA, R&D Systems), anti–mIGF-1 (catalog AF791, R&D Systems), anti-THBS4 (catalog AF2390, R&D Systems), and

(9) Periodontitis promotes bacterial extracellular vesicle-induced neuroinflammation in the brain and trigeminal ganglionPLOS PathogensOctober 23, 2023Jae Yeong Ha, Jiwon Seok, Suk-Jeong Kim, Hye-Jin Jung, Ka-Young Ryu, Michiko Nakamura, Il-Sung Jang, Su-Hyung Hong, Youngkyun Lee, Heon-Jin Leerespective antibodies. For primary immunostaining, anti–TNF-α (1:200, ab6671; Abcam), anti–IL-6 (1:200, AF406NA; R&D Systems), anti-GFAP (1:400, #3670s, Cell Signaling Technology, Danvers, MA, USA), and anti-NeuN

(10) Blockade of IL-6 signaling alleviates atherosclerosis in Tet2-deficient clonal hematopoiesisNat Cardiovasc ResJune 1, 2023Liu W, Yalcinkaya M, Maestre IF, Olszewska M, Ampomah PB, Heimlich JB, Wang R, Vela PS, Xiao T, Bick AG, et al.Labs); anti-Ki67 (1:100 dilution, catalog no. ab15580, Abcam); anti-IL-6 (1:100 dilution, catalog no. AF-406-NA, R&D Systems); anti-RPF (1:100 dilution, catalog no. 600-401-379, Rockland); anti-cleaved caspase 3

(11) MTHFD2 reprograms macrophage polarization by inhibiting PTENCell reportsMay 30, 2023Shang M, Ni L, Shan X, Cui Y, Hu P, Ji Z, Shen L, Zhang Y, Zhou J, Bing Chen, et al.

(12) Modulation of microbiome diversity and cytokine expression is influenced in a sex-dependent manner during agingFrontiers in MicrobiomesOctober 10, 2022Sarah E. Webster, Duncan Vos, Thomas L. Rothstein, Nichol E. HolodickIL-10 neutralization Isolated CD5+ B-1 cells were treated with 10 μg/ml of anti-IL-6 (R&D Systems AF-406-NA) and/or anti-IL-10 (R&D Systems AB-417-NA) neutralizing antibody for 24 hours at 37°C. As a control

(13) The study of direct and indirect effects of radiofrequency ablation on tumor microenvironment in liver tumor animal modelBMC cancerJune 17, 2022Jiang AN, Wang B, Wang S, Zhao K, Wu H, Yan K, Wu W, Yang W.

(14) CCN2 participates in overload-induced skeletal muscle hypertrophyMatrix biology : journal of the International Society for Matrix BiologyFebruary 1, 2022Petrosino JM, Longenecker JZ, Angell CD, Hinger SA, Martens CR, Accornero F.

(15) The inflammatory signalling mediator TAK1 mediates lymphocyte recruitment to lipopolysaccharide-activated murine mesenchymal stem cells through interleukin-6Molecular and cellular biochemistryOctober 1, 2021Oelze B, Elger K, Schadzek P, Burmeister L, Hamm A, Laggies S, Seiffart V, Gross G, Hoffmann A.µg/ml LPS or left untreated overnight. If applicable, a goat polyclonal IL-6 antibody (R&D systems AF-406-NA) or a normal goat IgG control antibody (R&D systems AB-108-C) were added (final concentration: 2 µg/ml).

(16) EphA2 Is a Clinically Relevant Target for Breast Cancer Bone Metastatic DiseaseJBMR plusApril 1, 2021Vaught DB, Merkel AR, Lynch CC, Edwards J, Tantawy MN, Hilliard T, Wang S, Peterson T, Johnson RW, Sterling JA, et al.kits for mouse and human IL‐6 were purchased from RayBiotech. Neutralizing anti‐mouse IL‐6 antibody (AF‐406‐NA), recombinant mouse and human IL‐6, and recombinant mouse ephrin‐A1‐Fc were purchased from R&D Systems.

(17) Targeted Disruption of Bone Marrow Stromal Cell-Derived Gremlin1 Limits Multiple Myeloma Disease Progression In VivoCancersAugust 3, 2020Clark KC, Hewett DR, Panagopoulos V, Plakhova N, Opperman KS, Bradey AL, Mrozik KM, Vandyke K, Mukherjee S, Davies GCG, et al.protein (cat #I9646-5UG, Sigma Aldrich) and 0.05 µg/mL neutralizing anti-mouse Il-6 antibody (cat #AF-406-NA, R&D systems, Minneapolis, MN, USA) were used in cultures as specified. 4.8. Luciferase Proliferation

(18) DAPT, a potent Notch inhibitor regresses actively growing abdominal aortic aneurysm via divergent pathwaysClinical science (London, England : 1979)June 26, 2020Hans CP, Sharma N, Dev R, Blain JM, Tonniges J, Agarwal G.For IHC, the abdominal aortae were stained with antibodies for F4/80 (1:200; ab100790, Abcam), Il6 (1:200, AF-406-NA, R&D Systems), cleaved caspase 3 (1:200; 9661S, Cell Signaling), Notch1 intracellular domain (NICD,

(19) The microRNA-34a-Induced Senescence-Associated Secretory Phenotype (SASP) Favors Vascular Smooth Muscle Cells CalcificationInternational journal of molecular sciencesJune 23, 2020Zuccolo E, Badi I, Scavello F, Gambuzza I, Mancinelli L, Macrì F, Tedesco CC, Veglia F, Bonfigli AR, Olivieri F, et al.blocked in 5% goat serum in PBS-T for 45 min at room temperature. Primary antibody against IL6 (5 μg/mL, AF-406-NA, R&D Systems) was dissolved in 1% goat serum PBS-T and incubated overnight at 4 °C in a humidified chamber.

(20) Vitronectin mitigates stroke-increased neurogenesis only in female mice and through FAK-regulated IL-6Experimental neurologyJanuary 1, 2020Jia C, Keasey MP, Malone HM, Lovins C, Hagg T.h after MCAO, 20 μm coronal frozen sections were stained with goat anti-mouse IL-6 antibody (1:100, AF-406-NA, R&D Systems, RRID:AB 354478) combined with rabbit anti-S100 (astrocytes, instead of lowly expressed

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IL-6 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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IL-6 Antibody from R&D Systems, a Bio-Techne Brand

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