CCL4/MIP-1 beta Antibody AF-451-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCCL4/MIP-1 beta Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $539.00Supplier Page View Company Product Page
Catalog NumberAF-451-NA
NCBI Gene AliasesAct-2, Scya4, G-26, ACT2, LAG-1, MIP-1B, LAG1, SCYA4, Mip1b, MIP1B1, MIP1B, SCYA2, HC21, MIP-1-beta, AT744.1
ApplicationsELISA Development,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Neutralization,Immunocytochemistry
TargetCCL4/MIP-1 beta
IsotypeIgG
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Conjugate/TagUnconjugated
ReactivityHuman,Mouse,Rat,Transgenic Mouse
Quantity100 ug (also 25 ug)
NCBI Full Gene NameC-C motif chemokine ligand 4, chemokine (C-C motif) ligand 4
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse CCL4/MIP-1ß, Ala24-Asn92, Accession # Q5QNV9
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects CCL4/MIP-1ß in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) MIP-1ß and recombinant mouse (rm) MIP-1 alpha is observed. This antibody will not neutralize the biological activity of rmMIP-1 alpha. In the chemotaxis assay this antibody will partially neutralize rhMIP-1 alpha and rhMIP-1ß at a 10-30 fold higher IgG concentration.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study microglial responses in the brain?

Yes, the CCL4/MIP-1 beta antibody has been utilized in studies examining microglial activation and responses in various contexts, including during brain injury and development. For instance, it was used in the study by Hammond et al. to analyze microglial states in the injured brain, highlighting its relevance in understanding microglial function and behavior.

Is this antibody effective for detecting CCL4 in immunohistochemistry?

The CCL4/MIP-1 beta antibody has been shown to work effectively in immunohistochemistry applications, as demonstrated in the study by Rosin et al., where it was used to stain embryonic hypothalamic microglia, indicating its utility in visualizing CCL4 expression in tissue samples.

How does this antibody perform in neutralization assays?

The CCL4/MIP-1 beta antibody can partially neutralize the biological activity of recombinant human MIP-1 alpha and MIP-1 beta, although this requires a significantly higher concentration of the antibody (10-30 fold) compared to typical usage levels. This characteristic can be important for experiments involving chemotaxis assays.

What species can this antibody be used with?

This antibody is reactive with mouse and rat samples, making it suitable for studies involving these species, particularly in the context of cytokine signaling and immune responses in the central nervous system.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) CSF1R+ macrophage and osteoclast depletion impairs neural crest proliferation and craniofacial morphogenesisDevelopmentAugust 15, 2026Felix Ma, Rose Ru Jing Zhou, Matthew Rosin, Iris Zhou, Sabrina Ownsworth, Rouzbeh Ostadsharif Ostadsharif Memar, Vincent B. Wong, Jessica M. Rosingoat anti-CCL3 (1:200; R&D Systems, AF-450-NA; RRID: AB_354492), goat anti-CCL4 (1:200; R&D Systems, AF-451-NA; RRID: AB_2071055) and/or mouse anti-Ki67 (1:200; BD Pharmingen, 556003; RRID: AB_396287) at 4°C overnight.

(2) CD4-mediated immunity shapes neutrophil-driven tuberculous pathologybioRxiv : the preprint server for biologyApril 15, 2024Gern BH, Klas JM, Foster KA, Cohen SB, Plumlee CR, Duffy FJ, Neal ML, Halima M, Gustin AT, Diercks AH, et al.

(3) Senescent Microglia Represent a Subset of Disease-Associated Microglia in P301S MiceJournal of Alzheimer’s DiseaseJanuary 31, 2024Pei Y. Ng, Cheng Zhang, Hu Li, Darren J. Bakerethylene glycol, 500 ml PBS) at −20°C. Iba1 staining (wako #019-19741) and Ccl4 staining (R&D systems, #AF-451-NA) were carried out on free-floating brain sections from bregma −2.78mm to −3.28mm as previously described

(4) IL9 Polarizes Macrophages to M1 and Induces the Infiltration of Antitumor Immune Cells via MIP-1 and CXCR3 ChemokinesCancer Res CommunJanuary 1, 2023Do-Thi VA, Park SM, Park SM, Jeong HJ, Cho G, An HJ, Kim YS, Lee H, Lee JO.

(5) Senescent microglia represent a subset of disease-associated microglia in P301S miceJ Alzheimers DisJanuary 1, 2023Pei Y. Ng, Cheng Zhang, Hu Li, Darren J. Bakerethylene glycol, 500 ml PBS) at −20°C. Iba1 staining (wako #019-19741) and Ccl4 staining (R&D systems, #AF-451-NA) were carried out on free-floating brain sections from bregma −2.78mm to −3.28mm as previously described

(6) Gestational Bisphenol A Exposure Impacts Embryonic Hypothalamic Microglia Numbers, Ramification, and Phagocytic CupsFrontiers in neuroscienceJanuary 1, 2022Rosin JM, Tretiakov N, Hanniman E, Hampton K, Kurrasch DM.Bio-Rad MCA1957), goat anti-CCL3 (1:200, R&D systems AF450-NA), or goat anti-CCL4 (1:200, R&D systems AF451-NA) at 4°C overnight, followed by secondary antibody incubation and staining with Hoechst 33342 (1:1,000,

(7) Memory CD8⁺ T cells mediate early pathogen-specific protection via localized delivery of chemokines and IFNγ to clusters of monocytesScience advancesSeptember 3, 2021Boutet M, Benet Z, Guillen E, Koch C, M'Homa Soudja S, Delahaye F, Fooksman D, Lauvau G.

(8) Radiation Triggers a Dynamic Sequence of Transient Microglial Alterations in Juvenile BrainCell reportsJune 2, 2020Osman AM, Sun Y, Burns TC, He L, Kee N, Oliva-Vilarnau N, Alevyzaki A, Zhou K, Louhivuori L, Uhlén P, et al.

(9) Recruitment of CD103⁺ dendritic cells via tumor-targeted chemokine delivery enhances efficacy of checkpoint inhibitor immunotherapyScience advancesDecember 1, 2019Williford JM, Ishihara J, Ishihara A, Mansurov A, Hosseinchi P, Marchell TM, Potin L, Swartz MA, Hubbell JA.at RT. Tissues were then stained with mouse anti-human CD31 (ab119339, Abcam), goat anti-mouse CCL4 (AF-451-NA, R&D Systems), and rabbit anti–collagen I antibody (ab34710, Abcam) for 1 hour at RT. After staining

(10) Single-Cell RNA Sequencing of Microglia throughout the Mouse Lifespan and in the Injured Brain Reveals Complex Cell-State ChangesImmunityJanuary 15, 2019Hammond TR, Dufort C, Dissing-Olesen L, Giera S, Young A, Wysoker A, Walker AJ, Gergits F, Segel M, Nemesh J, et al.for human staining,Sigma,cat#: HPA051870 Anti-CCL4/MIP-1 beta – for mouse staining,R&D Systems,cat#: AF-451-NA Anti-CCL4/MIP-1 beta – for human staining,R&D Systems,cat#: AF-271-NA HLA-DR,Abcam,cat#: ab166777 CD11b

(11) IFNβ Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain InjuryScientific reportsApril 20, 2017Thaney VE, O'Neill AM, Hoefer MM, Maung R, Sanchez AB, Kaul M.from R&D Systems (Minneapolis, MN) and reconstituted in sterile PBS: CCL3 (cat# AF450NA), CCL4 (cat# AF451NA), CCL5 (cat# AF478NA), CXCL10 (cat# AF466NA), IFNγ (cat# AF585NA). All antibodies were tested for neutralizing

(12) Identification of chemoattractive factors involved in the migration of bone marrow-derived mesenchymal stem cells to brain lesions caused by prionsJournal of virologyNovember 1, 2011Song CH, Honmou O, Furuoka H, Horiuchi M.IGF-1 receptor (IGF-1R; clone 33255), CXCR4 (clone 12G5), IGF-1 (AF791), CCL2 (clone 123602), CCL4 (AF-451-NA), CCL5 (AF-478), CCL7 (AF-456-NA), CCL17 (AF-529), CCL24 (clone 106521), CX3CL1 (clone 126315), CXCL10

(13) Comprehensive assessment of chemokine expression profiles by flow cytometryThe Journal of clinical investigationMarch 1, 2010Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D.

(14) CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis processJournal of immunology (Baltimore, Md. : 1950)November 1, 2008Wu Y, Li YY, Matsushima K, Baba T, Mukaida N.

(15) Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infectionThe Journal of experimental medicineSeptember 1, 2008Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, et al.

(16) CCL4 protects from type 1 diabetes by altering islet beta-cell-targeted inflammatory responsesDiabetesMarch 1, 2007Meagher C, Arreaza G, Peters A, Strathdee CA, Gilbert PA, Mi QS, Santamaria P, Dekaban GA, Delovitch TL.

(17) CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune responseInfection and immunityMay 1, 2003Cardona AE, Gonzalez PA, Teale JM.

(18) Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in ratsJournal of immunology (Baltimore, Md. : 1950)March 1, 2000Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, et al.

(19) Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in ratsJournal of immunology (Baltimore, Md. : 1950)March 1, 2000Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, et al.

(20) Interferon-driven CAF reprogramming augments immunogenic response to neoadjuvant radiotherapy in colorectal cancerCell reports. MedicineJanuary 1, 0001"Huang L, Lu W, Wu R, Li Y, Ou Z, Chen J, Liu Y, Yang W, Xue W, Mu P, et al."polyclonal antibody,eBiosciences™,Cat#PA5-114961; RRID: AB_2899597 CCL4 anti-mouse antibody,NOVUS,Cat#AF-451-NA; RRID: AB_2071055 CCL4 anti-human antibody,NOVUS,Cat#AF-271-NA; RRID: AB_354437 Phospho-STING (Ser366) (E9A9K) Rabbit mAb

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