IL-10 Antibody AF-217-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIL-10 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF-217-NA
NCBI Gene AliasesIL10A, TGIF, GVHDS, IL-10, CSIF
ApplicationsAssay Development,Mass Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Neutralization,Functional Assay,Imaging Mass Cytometry
HostGoat
TargetIL-10
IsotypeIgG
Conjugate/TagUnconjugated
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Primate - Macaca mulatta (Rhesus Macaque)
NCBI Full Gene Nameinterleukin 10
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10, Ser19-Asn178, Accession # P22301
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant Epstein-Barr virus IL-10 is observed and less than 20% cross-reactivity with recombinant mouse IL-10, recombinant feline IL-10, recombinant porcine IL-10, recombinant equine IL-10, and recombinant rat IL-10 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Pharmacological Characterization of GLPG3667, a Tyrosine Kinase 2-Selective Inhibitor, for the Treatment of Inflammatory and Autoimmune DiseasesInflammationMay 16, 2026Céline Cottereaux, May-Linda Lepage, Isabelle Parent, Maikel Colli, An Van de Water, Emilie Lagoutte, Christelle David, Maarten Van Balen, Adrien Cosson, Roland Blanque, et al.lipopolysaccharide (LPS) (L2654, Sigma-Aldrich, St. Louis, MI, USA) for 15 h. When neutralizing anti-IL-10 antibody (AF-217-NA, R&D Systems) was used, it was incubated with IL-10 for 30 min before the mixture was added to the monocytes.

(2) Multiplex spatial omics reveals changes in immune-epithelial crosstalk during inflammation and dysplasia development in chronic IBD patientsiScienceAugust 16, 2024Matthijs J.D. Baars, Evelien Floor, Neeraj Sinha, José J.M. ter Linde, Stephanie van Dam, Mojtaba Amini, Isaäc J. Nijman, Joren R. ten Hove, Julia Drylewicz, G.Johan A. Offerhaus, et al.D3A7),Cell Signaling Technology,Cat# CST9145BF; RRID: AB_2491009 IL-10 (polyclonal),R&D Systems,Cat# AF-217-NA; RRID: AB_354401 CD45 (clone D9M8I),Cell Signaling Technology,Cat# CST13917; RRID: AB_2750898 CD8a

(3) A keratinocyte-adipocyte signaling loop is reprogrammed by loss of BTG3 to augment skin carcinogenesisCell death and differentiationAugust 1, 2024Cheng YC, Acedera JD, Li YJ, Shieh SY.

(4) The role of extracellular vesicle fusion with target cells in triggering systemic inflammationNature communicationsFebruary 7, 2024Papareddy P, Tapken I, Kroh K, Varma Bhongir RK, Rahman M, Baumgarten M, Cim EI, Györffy L, Smeds E, Neumann A, et al.

(5) Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitisiScienceOctober 20, 2023van Eijs MJM, Ter Linde JJM, Baars MJD, Amini M, Laclé MM, Brand EC, Delemarre EM, Drylewicz J, Nierkens S, Verheijden RJ, et al.D9M8I),Cell Signaling Technology,Cat# CST13917BF; RRID: AB_2750898 IL-10 (polyclonal),R&D Systems,Cat# AF-217-NA; RRID: AB_354401 TIGIT (polyclonal),Abcam,Cat# ab233404; RRID: AB_2827380 CD8α (clone C8/144B),Thermo Fisher Scientific

(6) In vivo single-cell transcriptomics reveal Klebsiella pneumoniae skews lung macrophages to promote infectionEMBO molecular medicineDecember 7, 2022Dumigan A, Cappa O, Morris B, Sá Pessoa J, Calderon-Gonzalez R, Mills G, Lancaster R, Simpson D, Kissenpfennig A, Bengoechea JA.maintained throughout with either, 1 μg/ml of mouse monoclonal anti‐human IL‐10 antibody (R&D Systems. Ref: AF‐217‐NA), or equivalent concentrations of human IgG (Invitrogen) as control. To target IFNAR1/2, PMA differentiated

(7) Pomalidomide restores immune recognition of primary effusion lymphoma through upregulation of ICAM-1 and B7-2PLoS pathogensJanuary 1, 2021Shrestha P, Davis DA, Jaeger HK, Stream A, Aisabor AI, Yarchoan R.S3I-201 were purchased from Selleck Chemical (Houston, TX) and stored in DMSO. Anti-human IL10 antibody (cat# AF-217-NA) and recombinant human IL-10 protein (rIL-10) (cat# 217-IL010) were purchased from R&D systems (Minneapolis,

(8) A Novel Fusion of IL-10 Engineered to Traffic across Intestinal Epithelium to Treat ColitisJournal of immunology (Baltimore, Md. : 1950)December 1, 2020Fay NC, Muthusamy BP, Nyugen LP, Desai RC, Taverner A, MacKay J, Seung M, Hunter T, Liu K, Chandalia A, et al.membrane (Bio-Rad Laboratories) that was probed using a goat polyclonal Ab recognizing hIL-10 (1:1000 dilution, AF-217-NA; R&D Systems) and detected using an alkaline phosphatase–conjugated secondary rabbit anti-goat Ab (1:10,000

(9) Expression of Tim-1 in primary CNS lymphomaCancer medicineNovember 1, 2016Kishimoto W, Nishikori M, Arima H, Miyoshi H, Sasaki Y, Kitawaki T, Shirakawa K, Kato T, Imaizumi Y, Ishikawa T, et al.

(10) Porphyromonas gingivalis Outer Membrane Vesicles Induce Selective Tumor Necrosis Factor Tolerance in a Toll-Like Receptor 4- and mTOR-Dependent MannerInfection and immunityApril 1, 2016Waller T, Kesper L, Hirschfeld J, Dommisch H, Kölpin J, Oldenburg J, Uebele J, Hoerauf A, Deschner J, Jepsen S, et al.(R&D Systems, Minneapolis, MN, USA) were used at 10 ␮g/ml: goat anti-human IL-10 polyclonal Ab (catalog no. AF-217-NA), mouse anti-human TLR2 IgG2B monoclonal Ab (MAb) (catalog no. MAB2616), goat anti-human TLR4 polyclonal

(11) Role of Interleukin-10 on Nasal Polypogenesis in Patients with Chronic Rhinosinusitis with Nasal PolypsPloS oneJanuary 1, 2016Xu J, Han R, Kim DW, Mo JH, Jin Y, Rha KS, Kim YM.

(12) The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cellsLeukemiaJanuary 1, 2016Saulep-Easton D, Vincent FB, Quah PS, Wei A, Ting SB, Croce CM, Tam C, Mackay F.IL-10 was achieved with purified anti-human BAFF (AF124), anti-mouse BAFF (AF2106), anti-human IL-10 (AF-217-NA) and antimouse IL-10 (AF-417-NA) all from R&D Systems (Minneapolis, MN, USA) and used at 50μg/ml. In vitro

(13) Immune suppression and resistance mediated by constitutive activation of Wnt/β-catenin signaling in human melanoma cellsJournal of immunology (Baltimore, Md. : 1950)September 1, 2012Yaguchi T, Goto Y, Kido K, Mochimaru H, Sakurai T, Tsukamoto N, Kudo-Saito C, Fujita T, Sumimoto H, Kawakami Y.

(14) PLTP regulates STAT3 and NFκB in differentiated THP1 cells and human monocyte-derived macrophagesBiochimica et biophysica actaOctober 1, 2011Vuletic S, Dong W, Wolfbauer G, Tang C, Albers JJ.respectively) were from Abcam (Cambridge, MA). Anti-IL6 (Cat. No. AF-206-NA) and anti-IL10 antibodies (Cat. No. AF-217-NA), as well as IL1β, MIP1α and TGFβ ELISA plates (Cat. No. HSLB00C, DMA00 and DB100B, respectively) were

(15) Effect of fluticasone furoate on interleukin 6 secretion from adenoid tissues in children with obstructive sleep apneaArchives of otolaryngology--head & neck surgeryJune 1, 2011Esteitie R, Emani J, Sharma S, Suskind DL, Baroody FM.microwave for 10 minutes at maximal power. Goat anti–IL-10 antibody at a 1:20 dilution (catalog No. AF-217NA; R&D Systems) was applied on the tissue sections for 1 hour at room temperature. Antibody binding was

(16) IL-10 expression by primary tumor cells correlates with melanoma progression from radial to vertical growth phase and development of metastatic competenceModern pathology : an official journal of the United States and Canadian Academy of Pathology, IncJune 1, 2011Itakura E, Huang RR, Wen DR, Paul E, Wünsch PH, Cochran AJ.

(17) Induction of phenotype modifying cytokines by FERMT1 mutationsHuman mutationApril 1, 2011Heinemann A, He Y, Zimina E, Boerries M, Busch H, Chmel N, Kurz T, Bruckner-Tuderman L, Has C.available primary antibodies Antigen protein Clone / Company Application Reference Anti human IL-10 AF217NA R&D Systems IB Anti human IL-20 AF1102 R&D Systems IB Anti human IL-24 AF1965 R&D Systems IB Anti

(18) Irritant-induced migration of Langerhans cells coincides with an IL-10-dependent switch to a macrophage-like phenotypeThe Journal of investigative dermatologyFebruary 1, 2011Ouwehand K, Oosterhoff D, Breetveld M, Scheper RJ, de Gruijl TD, Gibbs S.

(19) Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humansBloodSeptember 23, 2010Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ.

(20) Direct and indirect impairment of human dendritic cell function by virulent Francisella tularensis Schu S4Infection and immunityJanuary 1, 2009Chase JC, Celli J, Bosio CM.

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IL-10 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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IL-10 Antibody from R&D Systems, a Bio-Techne Brand

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