TNF-alpha Antibody AF-210-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemTNF-alpha Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF-210-NA
NCBI Gene AliasesTNFSF2, DIF, TNF-alpha, TNLG1F, TNFA
ApplicationsAffinity Assay,Bioassay,ELISA Development,Immunohistochemistry,Western Blot,Neutralization,Immunocytochemistry,Functional Assay
IsotypeIgG
Conjugate/TagUnconjugated
ReactivityHuman,Mouse,Primate - Papio cynocephalus (Yellow Baboon)
HostGoat
TargetTNF-alpha
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
NCBI Full Gene Nametumor necrosis factor
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human TNF-alpha, Val77-Leu233, Accession # P01375
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human TNF-alpha in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Organoid phenotypic screening identified glycyrrhizin that confers protection against tumor necrosis factor-induced cell deathStem Cell ReportsMay 12, 2026Yu Takahashi, Zhongwei Zhang, Izumi Tanaka, I-Ting Lee, Jincheng He, Yurina Koura, Shintaro Sato, Hirotatsu Kojima, Takayoshi Okabe, Hiroshi Kiyono, et al.FITC anti-mouse EpCAM (118207) antibody was purchased from BioLegend. Anti-TNF neutralizing antibody (AF210-NA) was purchased from R&D Systems. Secondary antibodies were purchased from Jackson ImmunoResearch, except

(2) Therapeutic stress triggers tumor STAT1 acetylation to disarm immunotherapyCell Reports MedicineNovember 18, 2025Po-Hsien Chiu, Kuan-Chen Lai, Hung-Ling Wang, Yao-Wen Chang, Wen-Chi Wu, Tien-Hua Chen, Yu-Shuen Tsai, Jie-Hong Song, Nai-Yun Sun, Gar-Yang Chau, et al.IgG2a isotype control,Bio X Cell,Cat# BE0089;RRID:AB_1107769 Human TNF-α Antibody,R&D systems,Cat# AF-210-NA; Human IFN-β Antibody,R&D systems,Cat# AF814;RRID:AB_2122897 Rabbit polyclonal IFITM1 Antibody,Proteintech,Cat#

(3) Advanced High-Content Phenotypic Screening to Identify Drugs That Ameliorate the Inhibition of Skeletal Muscle Cell Differentiation Induced by Cancer Cachexia SerumPharmaceuticals (Basel)March 21, 2025Atsushi Nakane, Hiroyuki Nakagawa, Hidetaka NagataIn the TNFα neutralization test, anti-human TNFα neutralizing-antibody (R&D systems, Inc., USA, cat# AF-210-NA) was added to differentiation medium at a final concentration of 1 μg/mL [54,55] and preincubated for

(4) RIPK1 is required for ZBP1-driven necroptosis in human cellsPLoS biologyFebruary 1, 2025Amusan OT, Wang S, Yin C, Koehler HS, Li Y, Tenev T, Wilson R, Bellenie B, Zhang T, Wang J, et al.

(5) Effects of amyloid-β-mimicking peptide hydrogel matrix on neuronal progenitor cell phenotypeActa BiomaterialiaJuly 1, 2024Tess Grett Mathes, Mahsa Monirizad, Menekse Ermis, Natan Roberto de Barros, Marco Rodriguez, Heinz-Bernhard Kraatz, Vadim Jucaud, Ali Khademhosseini, Natashya Falcone(Invitrogen, A12379), and 4',6-diamidino-2-phenylindole (DAPI), the second group included TNF-α (R&D Systems, AF-210-NA) and ß-tubulin (SCT #60092.1), Phalloidin Alexa Flour 594 (Invitrogen, A12381) and DAPI. Finally, the

(6) Interleukin-10 induces TNF-driven apoptosis and ROS production in salivary gland cancer cellsHeliyonJune 15, 2024Maksym Skrypnyk, Tetiana Yatsenko, Oleksandra Riabets, Yousef Salama, Margarita Skikevych, Taro Osada, Morikuni Tobita, Satoshi Takahashi, Koichi Hattori, Beate Heissig575804, BioLegend, San Diego, California, USA), anti-human TNFa neutralizing antibody (Biotechne, #AF-210-NA), recombinant human TNFa (Biotechne, #210-TA), N-acetyl-l-cysteine (NAC; Cat. A7250, Sigma-Aldrich,

(7) TNFR2 Depletion Reduces Psoriatic Inflammation in Mice by Downregulating Specific Dendritic Cell Populations in Lymph Nodes and Inhibiting IL-23/IL-17 PathwaysThe Journal of investigative dermatologyAugust 1, 2022Chandrasekharan UM, Kaur R, Harvey JE, Braley C, Rai V, Lee M, de Windt N, Hsieh J, Jaini R, Bayik D, et al.case were cultured in the presence of control IgG or a TNF-α-neutralizing antibody (R&D systems, cat# AF-210-NA, 100 ng/ml) for 18 hours. The antibodies were removed from the supernatant by protein A/G agarose prior

(8) 3D ultrastructural analysis of α-granule, dense granule, mitochondria, and canalicular system arrangement in resting human plateletsResearch and practice in thrombosis and haemostasisJanuary 1, 2020Pokrovskaya ID, Yadav S, Rao A, McBride E, Kamykowski JA, Zhang G, Aronova MA, Leapman RD, Storrie B.TNF‐alpha: goat polyclonal antibodies directed against recombinant TNF‐α of human origin (R&D Systems, AF‐210‐NA, R&D Systems, Minneapolis, MN, USA), VWF: rabbit polyclonal antibodies directed against human VWF (Dako,

(9) Intravascular heavy chain-modification of hyaluronan during endotoxic shockBiochemistry and biophysics reportsMarch 1, 2019Ni K, Gill A, Cao D, Koike K, Schweitzer KS, Garantziotis S, Petrache I.

(10) Detection of aryl hydrocarbon receptor agonists in human samplesScientific reportsMarch 21, 2018Rothhammer V, Borucki DM, Kenison JE, Hewson P, Wang Z, Bakshi R, Sherr DH, Quintana FJ.

(11) Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodiesProceedings of the National Academy of Sciences of the United States of AmericaJanuary 30, 2018Jalili R, Horecka J, Swartz JR, Davis RW, Persson HHJ.

(12) Folate receptor 1 (FOLR1) targeted chimeric antigen receptor (CAR) T cells for the treatment of gastric cancerPloS oneJanuary 1, 2018Kim M, Pyo S, Kang CH, Lee CO, Lee HK, Choi SU, Park CH.

(13) Blockade of TNFα to Improve Human CD34+ Cell Repopulating Activity in Allogeneic Stem Cell TransplantationFrontiers in immunologyJanuary 1, 2018Senyuk V, Patel P, Mahmud N, Rondelli D.NY), rabbit anti-thymocyte globulin (rATG, Thymoglobulin, Genzyme, Cambridge, MA), anti-TNFα antibody (AF-210-NA) from R&D Systems (Minneapolis, MN). qRT-PCR CD34+ cells re-isolated on human CD34+ MicroBead Kit

(14) Human monocyte-derived macrophages inhibit HCMV spread independent of classical antiviral cytokinesVirulenceJanuary 1, 2018Becker J, Kinast V, Döring M, Lipps C, Duran V, Spanier J, Tegtmeyer PK, Wirth D, Cicin-Sain L, Alcamí A, et al.10 µg/ml mouse anti-human IFN-γ (R&D Systems, MAB2851), 10 µg/ml goat anti-human TNF-α (R&D Systems, AF-210-NA), or 20 µg/ml mouse anti-human IFNAR Chain 2 (PBL, 21385–1) were added. Every 2–3 d one fourth of the

(15) Replication defective viral genomes exploit a cellular pro-survival mechanism to establish paramyxovirus persistenceNature communicationsOctober 6, 2017Xu J, Sun Y, Li Y, Ruthel G, Weiss SR, Raj A, Beiting D, López CB.pre-treated with neutralizing antibody against TNFα, TNFR1, and/or TNFR2 (all from R&D system, cat. nos. AF-210-NA, MAB225, and AF726, respectively) at 2 µg/ml/antibody or with IgG control antibody (R&D system, cat.

(16) Changes in podocyte TRPC channels evoked by plasma and sera from patients with recurrent FSGS and by putative glomerular permeability factorsBiochimica et biophysica acta. Molecular basis of diseaseSeptember 1, 2017Kim EY, Roshanravan H, Dryer SE.(DUP00) were obtained from R&D Systems. Antibodies used for neutralization of suPAR (AF-807) and TNF (AF-210-NA) in a nephrotic plasma sample were from R&D Systems. 2.5. Electrophysiology Methods for making whole-cell recordings

(17) Promising Role of Toll-Like Receptor 8 Agonist in Concert with Prostratin for Activation of Silent HIVJournal of virologyFebruary 15, 2017Rochat MA, Schlaepfer E, Speck RF.CD86 (Biolegend [305201 and 305401]) were applied at 5 ␮g/ml, and inﬂiximab was obtained from R&D Systems (AF-210-NA) and was used at 1 ␮g/ml for the TNF blocking experiments. The ImmunoCult human CD3/CD28/CD2 T-cell activator

(18) Neutrophil Necroptosis Is Triggered by Ligation of Adhesion Molecules following GM-CSF PrimingJournal of immunology (Baltimore, Md. : 1950)November 15, 2016Wang X, He Z, Liu H, Yousefi S, Simon HU.Millipore Corporation (Billerica, MA). Anti-TNFR1 (mAb225), antiTNFR2 (mAb226), and anti-TNF-a mAb (AF-210-NA) were from R&D Systems Europe (Abingdon, U.K.). Control F(ab9)2 fragments (anti-CD16, anti-CD32, and

(19) Cytokines and Cytokine Receptors Involved in the Pathogenesis of Alzheimer's DiseaseJournal of clinical & cellular immunologyAugust 1, 2016Nagae T, Araki K, Shimoda Y, Sue LI, Beach TG, Konishi Y.7 of 15 Mature sTNFα and precursor mTNFα were recognized with the anti-TNFα antibody (R&D Systems, AF210NA) as positive bands at approximately 17 kDa and 26 kDa [174], respectively, on Western blots (Figure

(20) Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic PatientsPloS oneJanuary 1, 2016Savill SA, Leitch HF, Harvey JN, Thomas TH.Human Recombinant Interleukin-1α (IL-1α) (200-LA-002, R&D Systems), 500 ng/ml Human TNFα antibody (AF-210-NA, R&D Systems) and 1 μg/ml Human IL-1α antibody (AF-200-NA, R&D Systems). Cells were incubated for 24

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TNF-alpha Antibody

TNF-alpha Antibody

TNF-alpha Antibody

TNF-alpha Antibody

TNF-alpha Antibody

TNF-alpha Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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TNF-alpha Antibody from R&D Systems, a Bio-Techne Brand

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