Western blot was performed using Anti-KCC2 Polyclonal Antibody (Product # PA5-69278) and ~126 kDa band corresponding to KCC2 was observed in Mouse Brain and Rat Brain, but not in Mouse Stomach and Rat Stomach as reported An uncharacterized band at ~40 kDa was observed in the Brain tissues tested. Tissue lysate (40 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Stomach (Lane 3), and Rat Stomach (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The bot was probed with the primary antibody (1:1,000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).