FIH-1/HIF-1AN Antibody

Product Specs
ItemFIH-1/HIF-1AN Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $429.00Supplier Page View Company Product Page
Catalog NumberNB100-428
Quantity0.1 ml (also 0.025 ml)
ApplicationsImmunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Electron Microscopy, Knockdown Validated,Immunocytochemistry/ Immunofluorescence
Concentration1.0 mg/ml
Conjugate/TagUnconjugated
FormatTris-Citrate/Phosphate (pH 7.0 - 8.0)
PurityImmunogen affinity purified
ReactivityHuman,Mouse,Rat
TargetFIH-1/HIF-1AN
HostRabbit
Molecular Weight40.3 kDa
IsotypeIgG
Antibody TypePrimary Antibodies
ImmunogenA synthetic peptide made to the C-terminal region of human Factor Inhibiting HIF-1 (between residues 300 and the C-terminus). [UniProt# Q9NWT6]
NCBI Gene AliasesFIH1
NCBI Full Gene Namehypoxia inducible factor 1 subunit alpha inhibitor
ClonalityPolyclonal
Research AreaAngiogenesis, Cancer, Chromatin Research, Hypoxia, Transcription Factors and Regulators
StorageStore at 4C. Do not freeze
ValidationKnockout Validated

Specifications/Features

Available conjugates: Unconjugated

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Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Enhanced BPGM/2,3-DPG pathway activity suppresses glycolysis in hypoxic astrocytes via FIH-1 and TET2Brain research bulletinJanuary 1, 2023E G, Sun B, Liu B, Xu G, He S, Wang Y, Feng L, Wei H, Zhang J, Chen J, et al.

(2) Knockout of Factor-Inhibiting HIF (Hif1an) in Colon Epithelium Attenuates Chronic Colitis but Does Not Reduce Colorectal Cancer in MiceJournal of immunology (Baltimore, Md. : 1950)March 1, 2022Schützhold V, Gravemeyer J, Bicker A, Hager T, Padberg C, Schäfer J, Wrobeln A, Steinbrink M, Zeynel S, Hankeln T, et al.

(3) FIH-1-modulated HIF-1α C-TAD promotes acute kidney injury to chronic kidney disease progression via regulating KLF5 signalingActa pharmacologica SinicaDecember 1, 2021Li ZL, Wang B, Lv LL, Tang TT, Wen Y, Cao JY, Zhu XX, Feng ST, Crowley SD, Liu BC.

(4) Mint3 is dispensable for pancreatic and kidney functions in miceBiochemistry and biophysics reportsDecember 1, 2020Chung Y, Saitoh Y, Hayashi T, Fukui Y, Terada N, Seiki M, Murakami Y, Sakamoto T.buffer,1:100 Aquaporin 1 (rabbit),Merck Millipore,AB2219,citrate buffer,1:100 FIH-1 (rabbit),Novus Biologicals,NB100-428,none,1:100 List of antibodies used in this study.

(5) miR-125 family regulates XIRP1 and FIH in response to myocardial infarctionPhysiological genomicsAugust 1, 2020Williams AL, Khadka VS, Anagaran MCT, Lee K, Avelar A, Deng Y, Shohet RV.

(6) Oxygen-dependent asparagine hydroxylation of the ubiquitin-associated (UBA) domain in Cezanne regulates ubiquitin bindingThe Journal of biological chemistryFebruary 21, 2020Mader J, Huber J, Bonn F, Dötsch V, Rogov VV, Bremm A.

(7) Influence of 2-Methoxyestradiol and Sex on Hypoxia-Induced Pulmonary Hypertension and Hypoxia-Inducible Factor-1-αJournal of the American Heart AssociationMarch 5, 2019Docherty CK, Nilsen M, MacLean MR.

(8) Myc-induced nuclear antigen constrains a latent intestinal epithelial cell-intrinsic anthelmintic pathwayPloS oneJanuary 1, 2019Pillai MR, Mihi B, Ishiwata K, Nakamura K, Sakuragi N, Finkelstein DB, McGargill MA, Nakayama T, Ayabe T, Coleman ML, et al.with rabbit polyclonal antibodies against Mina (Invitrogen, cat. No. 40–9500) and FIH (Novus, cat. No. NB100-428). Statistical analysis GraphPad Prism software (version 7.0; GraphPad Software Inc.) was used to perform

(9) Interactome of the Autoimmune Risk Protein ANKRD55Frontiers in immunologyJanuary 1, 2019Ugidos N, Mena J, Baquero S, Alloza I, Azkargorta M, Elortza F, Vandenbroeck K.anti-PRKDC mouse monoclonal (Cat. No. NBP2-22128SS; 1:1,000) and anti-HIF1AN rabbit polyclonal (Cat. No. NB100-428; 1:1,000) from Novus Biologicals and subsequently with HRP-conjugated anti-rabbit (Cat. No. 111-035-144;

(10) Inhibition of pH regulation as a therapeutic strategy in hypoxic human breast cancer cellsOncotargetJune 27, 2017Meehan J, Ward C, Turnbull A, Bukowski-Wills J, Finch AJ, Jarman EJ, Xintaropoulou C, Martinez-Perez C, Gray M, Pearson M, et al.HIF-2α [Novus Biologicals, NB100-122, 1:1000], Factor inhibiting HIF-1α antibody [Novus Biologicals, NB100-428, 1:1000], anti-MMP14 antibody [Abcam, ab51074, 1:4000]; anti-α tubulin [Abcam, ab7291, 1:10000], anti-β

(11) Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1Science signalingMay 31, 2016Bullen JW, Tchernyshyov I, Holewinski RJ, DeVine L, Wu F, Venkatraman V, Kass DL, Cole RN, Van Eyk J, Semenza GL.

(12) NECAB3 Promotes Activation of Hypoxia-inducible factor-1 during Normoxia and Enhances Tumourigenicity of Cancer CellsScientific reportsMarch 7, 2016Nakaoka HJ, Hara T, Yoshino S, Kanamori A, Matsui Y, Shimamura T, Sato H, Murakami Y, Seiki M, Sakamoto T.

(13) Factor-inhibiting HIF-1 (FIH-1) is required for human vascular endothelial cell survivalFASEB journal : official publication of the Federation of American Societies for Experimental BiologyJuly 1, 2015Kiriakidis S, Henze AT, Kruszynska-Ziaja I, Skobridis K, Theodorou V, Paleolog EM, Mazzone M.

(14) Oxygen-dependent hydroxylation by FIH regulates the TRPV3 ion channelJournal of cell scienceJanuary 15, 2015Karttunen S, Duffield M, Scrimgeour NR, Squires L, Lim WL, Dallas ML, Scragg JL, Chicher J, Dave KA, Whitelaw ML, et al.

(15) Expression and DNA methylation levels of prolyl hydroxylases PHD1, PHD2, PHD3 and asparaginyl hydroxylase FIH in colorectal cancerBMC cancerNovember 6, 2013Rawluszko AA, Bujnicka KE, Horbacka K, Krokowicz P, Jagodziński PP.reagents Rabbit polyclonal (Rp) anti-PHD1 (NB100-310), -PHD2 (NB100-137), -PHD3 (NB100-139) and -FIH (NB100-428) antibodies (Ab) were provided by Novus Biologicals (Cambridge, UK). Rp anti-GAPDH Ab (FL-335) and goat anti-rabbit

(16) Factor inhibiting HIF-1 (FIH-1) modulates protein interactions of apoptosis-stimulating p53 binding protein 2 (ASPP2)Journal of cell scienceJune 15, 2013Janke K, Brockmeier U, Kuhlmann K, Eisenacher M, Nolde J, Meyer HE, Mairbäurl H, Metzen E.

(17) MT1-MMP plays a critical role in hematopoiesis by regulating HIF-mediated chemokine/cytokine gene transcription within niche cellsBloodJune 7, 2012Nishida C, Kusubata K, Tashiro Y, Gritli I, Sato A, Ohki-Koizumi M, Morita Y, Nagano M, Sakamoto T, Koshikawa N, et al.

(18) Increased activation of the hypoxia-inducible factor pathway in varicose veinsJournal of vascular surgeryMay 1, 2012Lim CS, Kiriakidis S, Paleolog EM, Davies AH.

(19) Factor inhibiting HIF (FIH) recognizes distinct molecular features within hypoxia-inducible factor-α (HIF-α) versus ankyrin repeat substratesThe Journal of biological chemistryMarch 16, 2012Wilkins SE, Karttunen S, Hampton-Smith RJ, Murchland I, Chapman-Smith A, Peet DJ.

(20) The chromatin remodeler ISWI regulates the cellular response to hypoxia: role of FIHMolecular biology of the cellNovember 1, 2011Melvin A, Mudie S, Rocha S.Santa Cruz Biotechnology, Santa Cruz, CA), hSNF2h (A301-017A; Bethyl Laboratories, Montgomery, AL), FIH (NB100-428; Novus Biologicals), HIF-1β (3718; Cell Signaling), proliferating cell nuclear antigen (PCNA; P8825;

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