TERT Antibody (2C4)

Product Specs
ItemTERT Antibody (2C4) - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberNB100-317
ImmunogenFull-length recombinant human Telomerase reverse transcriptase (TERT) from insect cells. [Uniprot: O14746]
Quantity0.1 ml (also 0.025 ml)
IsotypeIgM
ClonalityMonoclonal
Conjugate/TagUnconjugated
Clone2C4
FormatPBS
ApplicationsChemotaxis,IF/IHC,Chromatin Immunoprecipitation,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry/ Immunofluorescence
TargetTERT
HostMouse
ReactivityHuman,Mouse,Rat
PurityIgM purified
Molecular Weight127 kDa
Research AreaAutophagy, Cancer, Cellular Markers, Chromatin Research, DNA Repair, Stem Cell Markers
NCBI Full Gene Nametelomerase reverse transcriptase
NCBI Gene AliasesTP2, DKCB4, hTRT, PFBMFT1, CMM9, EST2, hEST2, TRT, DKCA2, TCS1
Concentration1.0 mg/ml
Antibody TypePrimary Antibodies
StorageAliquot and store at -20C or -80C. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this TERT antibody be used for immunohistochemistry in human tissues?

Yes, the TERT antibody (2C4) has been utilized in immunohistochemical analyses of human kidney tumor samples from patients with Beckwith-Wiedemann syndrome, demonstrating its applicability in this context .

Is there any evidence supporting the use of this antibody in studies related to macrophage polarization?

The TERT antibody has been employed in research investigating the role of telomerase reverse transcriptase in macrophage polarization during alcoholic liver disease, indicating its relevance in studies focused on immune cell activation and signaling pathways .

What types of cells have been shown to express TERT when using this antibody?

The TERT antibody has been used to confirm the expression of telomerase reverse transcriptase in telomerase-immortalized human multi-lineage progenitor cells differentiated to alveolar type 2-like cells, highlighting its utility in studies of respiratory cell biology .

Does this antibody have any documented applications in cancer research?

Yes, the TERT antibody has been referenced in studies examining the overexpression of TERT in tumorigenesis associated with Beckwith-Wiedemann syndrome, suggesting its potential role in cancer research .

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Telomerase mRNA-Lipid nanoparticles attenuate neuroinflammation after traumatic brain injury in micebioRxivDecember 26, 2025Goknur Kara, Morgan Holcomb, Anjana Tiwari, Hannah Flinn, Trinity Eimer, Austin Marshall, Marissa Burke, Peter Park, Karem Court, John P Cooke, et al.(Wako, cat. #019–19741) at 1:500 to label microglia and macrophages, and anti-mouse TERT (Novus, cat. #NB100–317) at 1:500. The following day, the sections were washed three times for 5 min each in PBS-T and then

(2) TELS1 stabilizes t-loops independently of TRF2 and controls telomere length in pluripotent cellsCell reportsSeptember 23, 2025Dufourd J, Huynh THY, Sauzet S, Lai QH, Abagnale F, Zitouni S, Giraud-Panis MJ, Urbach S, Gilson E, Tardat M, et al.

(3) Dynamic Telomere Length Response to Neurodevelopmental Arsenic Exposure: Insights into Transcriptional Regulation and Neuronal MorphogenesisEnviron Health (Wash)September 19, 2025Tian C, Wu Q, Chen F, Wu Y, Wang J, Zhang A, Wang W.°C). After blocking with 3% BSA and 0.1% Tween-20 (1 h, room temperature), primary antibodies TERT (NB100317, NOVUS, USA), BrdU (5292), Nestin (89529), DCX (556130), MAP2 (8707), and GFAP (12389), all purchased

(4) The NRF2-dependent transcriptional axis, XRCC5/hTERT drives tumor progression and 5-Fu insensitivity in hepatocellular carcinomaMolecular therapy oncolyticsMarch 17, 2022Liu T, Long Q, Li L, Gan H, Hu X, Long H, Yang L, Pang P, Wang S, Deng W.Cell Signaling Technology (Danvers, MA), while the hTERT antibody was purchased from Novus Biologicals (NB100-317). Streptavidin-agarose pull-down assay Briefly, a biotin-labeled, double-stranded DNA probe corresponding

(5) Differentiation of immortalized human multi-lineage progenitor to alveolar type 2-like cells: angiotensin-converting enzyme 2 expression and binding of severe acute respiratory syndrome coronavirus 2 spike and spike 1 proteinsCytotherapyDecember 1, 2021Collins DP, Osborn MJ, Steer CJ.594 (FAB9332T) were obtained from R&D Systems (Minneapolis, MN, USA). Antibodies specific for TERT (NB100-317) and caveolin-1 (CAV1) (NBP2-80617) were obtained from Novus Biologicals (Littleton, CO, USA). Antibodies

(6) TOP2A/MCM2, p16^INK4a, and cyclin E1 expression in liquid-based cytology: a biomarkers panel for progression risk of cervical premalignant lesionsBMC cancerJanuary 7, 2021Del Moral-Hernández O, Hernández-Sotelo D, Alarcón-Romero LDC, Mendoza-Catalán MA, Flores-Alfaro E, Castro-Coronel Y, Ortiz-Ortiz J, Leyva-Vázquez MA, Ortuño-Pineda C, Castro-Mora W, et al.

(7) Exosomal miR-1255b-5p targets human telomerase reverse transcriptase in colorectal cancer cells to suppress epithelial-to-mesenchymal transitionMolecular oncologyOctober 1, 2020Zhang X, Bai J, Yin H, Long L, Zheng Z, Wang Q, Chen F, Yu X, Zhou Y.

(8) In vitro Differentiation of Human TERT-Transfected Multi-Lineage Progenitor Cells (MLPC) into Immortalized Hepatocyte-Like CellsHepatic medicine : evidence and researchJanuary 1, 2020Collins DP, Hapke JH, Aravalli RN, Steer CJ.www.dovepress.com DovePress glucuronosyltransferase isoforms UGT1A1 and UGT2B7 (ab126269 and ab194697 from Abcam, Cambridge, MA), and TERT (NB100-317 from Novus, Littleton, CO) were used to label cells for 40 minutes. Unbound antibody was removed by

(9) Fisetin Promotes Hair Growth by Augmenting TERT ExpressionFrontiers in cell and developmental biologyJanuary 1, 2020Kubo C, Ogawa M, Uehara N, Katakura Y.were first stained with primary antibodies (anti-Ki-67, #12202, Cell Signaling Technology; anti-TERT, NB100-317, Novus Biologicals, Co., United States; anti-active-β-catenin, Merck Millipore, and anti-CD34, ab81289,

(10) Soybean extract increases telomerase reverse transcriptase protein expression in pancreatic β-cells of diabetes mellitus-induced ratsMedical Journal of IndonesiaOctober 4, 2019Muhammad Samsul Mustofa, Franciscus Dhyanagiri Suyatna, Mohamad Sadikin, Dwi Ari Pujianto, Aan Royhan, Kenconoviati Suwardjiincubated in normal serum for 30 min and rinsed with PBS solution 3 times, incubated with anti-TERT antibody (NB100-317, Novus Biologicals LLC) in a refrigerator overnight, and were rinsed again with PBS 3 times. The specimens

(11) Telomerase Plays a Pivotal Role in Collateral Growth Under Ischemia by Suppressing Age-Induced Oxidative Stress, Expression of p53, and Pro-Apoptotic ProteinsInternational heart journalMay 30, 2019Kokubun T, Saitoh SI, Miura S, Ishida T, Takeishi Y.(Cell signaling Technology, Inc. 1:1000), anti-Bcl-2 (#2876) (Cell signaling Technology, Inc. 1:1000), anti-TERT (NB100-317) (Novus Biologicals, LLC., Littleton, CO, USA, 1:1000), anti-HIF1α (#14179) (Cell signaling Technology,

(12) Phase I trial of intravenous Ad5CRT in patients with liver metastasis of gastrointestinal cancersCancer gene therapyMay 1, 2019Lee SJ, Shin SP, Lee SH, Kang JW, Kook MC, Kim IH, Kim HK.

(13) Generation of an immortalized human choroid endothelial cell line (iChEC-1) using an endothelial cell specific promoterMicrovascular researchMay 1, 2019Giacalone JC, Miller MJ, Workalemahu G, Reutzel AJ, Ochoa D, Whitmore SS, Stone EM, Tucker BA, Mullins RF.(Abeam; Cat. No. ab33168), anti-CFH (Abeam; Cat. No. ab8842), anti-hTERT (Novus Biological; Cat. No. NB100-317), and anti-SV40 TAg (Abeam; Cat. No. abl6879). To detect primary antibodies, Alexa Fluor 488 donkey

(14) Increased expression of NAF1 contributes to malignant phenotypes of glioma cells through promoting protein synthesis and associates with poor patient survivalOncogenesisApril 1, 2019Wei J, Yang Q, Shi J, Shi B, Ji M, Hou P.

(15) Stabilization of Reversed Replication Forks by Telomerase Drives Telomere CatastropheCellJanuary 25, 2018Margalef P, Kotsantis P, Borel V, Bellelli R, Panier S, Boulton SJ.anti-PCNA,Santa Cruz Biotechnology,Cat# sc-56; RRID: AB_628110 Mouse Monoclonal anti-TERT,Novus Biologicals,Cat#NB100-317; RRID: AB_2201588 Mouse Monoclonal anti-Tubulin,Sigma-Aldrich,Cat#T6074; RRID: AB_477582 Peroxidase-conjugated

(16) Telomere homeostasis in placentas from pregnancies with uncontrolled diabetesPlacentaAugust 1, 2016Biron-Shental T, Liberman M, Elbaz M, Laish I, Sharony R, Amiel A.

(17) TGF-β/β2-spectrin/CTCF-regulated tumor suppression in human stem cell disorder Beckwith-Wiedemann syndromeThe Journal of clinical investigationFebruary 1, 2016Chen J, Yao ZX, Chen JS, Gi YJ, Muñoz NM, Kundra S, Herlong HF, Jeong YS, Goltsov A, Ohshiro K, et al.standard procedures. Sections were stained with CTCF (Cell Signaling Technology, 2899) and TERT (NOVUS, NB100-317) antibodies. Diaminobenzidine was used as a chromogen, and hematoxylin was used for counterstaining.

(18) Telomerase reverse transcriptase acts in a feedback loop with NF-κB pathway to regulate macrophage polarization in alcoholic liver diseaseScientific reportsJanuary 4, 2016Wu XQ, Yang Y, Li WX, Cheng YH, Li XF, Huang C, Meng XM, Wu BM, Liu XH, Zhang L, et al.diluted in TBS/Tween-20 (0.075%) containing 3% Marvel. Mouse monoclonal antibody recognizing TERT (NB100-317, Novus Biologicals, Littleton, CO, USA) was used 1:500 as was anti-β-actin. Rabbit monoclonal antibody

(19) Effects of smoking on human telomerase reverse transcriptase expression in the skinInternational journal of dermatologyOctober 1, 2014Lotfi RA, El Zawahry KM, Kamar ZA, Hashem Z.

(20) Universal cancer peptide-based therapeutic vaccine breaks tolerance against telomerase and eradicates established tumorClinical cancer research : an official journal of the American Association for Cancer ResearchNovember 15, 2012Dosset M, Godet Y, Vauchy C, Beziaud L, Lone YC, Sedlik C, Liard C, Levionnois E, Clerc B, Sandoval F, et al.

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