DYKDDDDK Tag Antibody PA1-984B from Thermo Fisher Scientific

Product Specs
ItemDYKDDDDK Tag Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA1-984B
ApplicationsImmunocytochemistry, Western Blot
FormatPBS with 1mg/mL BSA and 0.05% sodium azide
Quantity200 µg
Conjugate/TagUnconjugated
ReactivityTag
ClonalityPolyclonal
TargetDYKDDDDK Tag
HostRabbit
Concentration1 mg/mL
ImmunogenSynthetic Peptide: D Y K D D D D K C
IsotypeIgG
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
PurityAntigen affinity chromatography
Antibody TypePrimary
Storage-20°C, Avoid Freeze/Thaw Cycles

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Website: www.thermofisher.com

(1) Deletion of Mitochondrial Calcium Uniporter Enhances Calcium Signals by Slowing Calcium Clearance and Triggers Adaptive Transcriptomic RemodelingJournal of Biological ChemistryApril 17, 2026Rajesh Bhardwaj, Abdull J. Massri, Gary S. Bird, Anant B. Parekhresidues 47–152, #HPA016480, Millipore Sigma), rabbit polyclonal anti-DYKDDDDK (FLAG) tag (1:500; #PA1-984B, Invitrogen), and rabbit monoclonal anti-vinculin (1:1000; #13901, Cell Signaling) as loading control.

(2) G protein Gαq subunits engage targets in the nucleus involved in chromatin remodeling and gene expressionJournal of Biological ChemistryApril 1, 2026Joseph Loomis, Naincy Chandan, Michael Burroughs, Saji Abraham, Rongxi Zhang, Yiyi Cao, Matthew J. Brody, Gregory G. Tall, Alan V. Smrckaused: V5 (Cell Signaling, D3H8Q), HA (Cell Signaling, C29F4), LgBiT (Promega, N7100), FLAG (Invitrogen, PA1-984B), GST (Invitrogen, MA4-004), and Streptavidin-IRDye800 (LI-COR, 925–32230). The following secondary

(3) X-linked cancer-associated polypeptide (XCP) from lncRNA1456 modulates PHF8 histone demethylase activity to regulate the epigenome, gene expression, and cellular pathways in breast cancerOncogeneApril 1, 2026Shrikanth S. Gadad, Cristel V. Camacho, Xuan Gong, Micah Thornton, Venkat S. Malladi, Anusha Nagari, Aishwarya Sundaresan, Tulip Nandu, Sneh Koul, Yan Peng, et al.(Abcam, ab13970; RRID:AB_300798), FLAG M2 (Sigma, F3165; RRID:AB_259529), DYKDDDDK Tag (Invitrogen, PA1-984B RRID:AB_347227), β-tubulin (Abcam, ab6046); RRID:AB_2210370, His Tag (H-3) (Santa Cruz, sc-8036; RRID:AB_627727),

(4) Xylosyltransferase engineering to manipulate proteoglycans in mammalian cellsNature Chemical BiologyApril 1, 2026Zhen Li, Himanshi Chawla, Lucia Di Vagno, Aisling Ní Cheallaigh, Meg Critcher, Douglas Sammon, Edgar Gonzalez-Rodriguez, David C. Briggs, Nara Chung, Vincent Chang, et al.(LI-COR Biosciences). FLAG-tagged decorin was visualized with a rabbit polyclonal antibody (Invitrogen, PA1-984b) and a 700CW anti-rabbit antibody (LI-COR Biosciences). In vitro glycosylation of recombinant, GAG-free

(5) Redox regulation of cell migration via Nischarin S-glutathionylationFree Radical Biology and MedicineFebruary 1, 2026Madhu C. Shivamadhu, Dhanushika S.K. Kukulage, Rayavarapu Padmavathi, Daniel Oppong, Faezeh Mashhadi Ramezani, Denaye N. Eldershaw, Brett M. Collins, Young-Hoon Ahnbuffer (300 μL) overnight at 4 °C. Primary antibodies include rabbit FLAG (1:500; Thermo Fischer, Cat# PA1–984B) and mouse Rac1 antibody (1:500; Thermo Fischer, clone 23A8 #05–389). The next day, the antibody solution

(6) Targeting and dissociating HIF2α from the molecular chaperone Hsp70 triggers apoptosis in kidney cancerCommunications MedicineJanuary 12, 2026Jennifer A. Heritz, Sarah J. Backe, Angela Pacherille, Sara J. Cayaban, Michael F. Basin, Britannia Smith, Rebecca A. Sager, Michael Daneshvar, Dawn E. Post, Mark R. Woodford, et al.were detected with antibodies recognizing: 1:4,000 Rabbit anti-FLAG tag (ThermoFisher Scientific, Cat# PA1-984B; RRID:AB_347227), 1:4,000 Rabbit anti-HA tag (C29F4) (Cell Signaling Technology, Cat# 3724; RRID:AB_1549585),

(7) Cancer histone H2A.Z missense mutations disrupt function through distinct local and allosteric effectsResearch SquareJanuary 12, 2026Maria Aristizabalwere analyzed by SDS-PAGE and immunoblotted with anti-Pgk1 (Cat. Ab113687, Abcam), anti-FLAG (Cat. PA1-984B, ThermoFisher SCITIFIC), and anti-H4 (Cat. Ab31830, Abcam) antibodies. Co-immunoprecipitation Overnight

(8) Regionally mapped astrocytic responses to cortical and white matter stroke show differential roles in astrocyte-induced vascular remodelingNeuronDecember 17, 2025Amy J. Gleichman, Riki Kawaguchi, Elle M. Rathbun, Michael V. Sofroniew, S. Thomas Carmichaelguinea pig anti-Glt1 (Frontier Institute, #GLT1-GP, 1:200), rabbit anti-FLAG (Thermo Fisher Scientific, #PA1-984B, 1:400), chicken anti-V5 (Abcam, #ab9113, 1:500), human anti-V5 (Absolute Antibody, #Ab00136-10.0, 1:400),

(9) Polarity protein Par3L deletion causes chromosomal segregation defects and tumorigenesisJournal of Biological ChemistryNovember 20, 2025Weili Zhang, Fengyan Dai, Yindan Hong, Chenyue Gao, Jiangchao Li, Lijun Dai, Yuxiang Liang, Yi Zhong, Yongliang Huoanti Myc,Proteintech,16286-1-AP,1:1000 Ms anti c-Myc,Santa Cruz,sc-40,1:1000 Rb anti DYKDDDDK,Thermo,PA1-984B,1:1000 for WB 1 μg/ml for IF Gt anti Ms AF Plus 488,Invitrogen,A32723,1:1000 Gt anti Ms AF Plus 555,Invitrogen,A32727,1:1000 Gt

(10) Mice carrying the human dopamine D2 receptor pathogenic mutation p.Met374Arg exhibit hyperactivity and aberrant D2 receptor functionMolecular PharmacologyNovember 1, 2025Dayana Rodriguez-Contreras, Joseph J. Lebowitz, Cheryl Reed, Elizabeth Walker-Ziegler, David C. Buck, Guochuan Li, Shu Xie, Yulong Li, Lev M. Fedorov, Tamara J. Phillips, et al.Sigma-Aldrich. The level of surface FLAG-tagged D2 receptor was assessed by probing with an anti-FLAG antibody (Cat. PA1-984B; Fisher Scientific) diluted 1:4,500 in TBS plus 1 mM CaCl2 and 1% BSA for 1 h at 37°C. The anti-FLAG

(11) Tdrd15 is dispensable for male fertility and spermatogenesis in the golden hamsterEur J Med ResOctober 22, 2025Miao Zhu, Yijian Xiang, Haodong Wang, Shanmeizi Zhao, Wentao Zeng, Jianmin Li, Bing Yao(Proteintech, 16286-1-AP, rabbit, 1:1000) to detect Myc-TDRD15, and anti-Flag antibody (Thermo Fisher, PA1-984B, rabbit, 1:1000) to confirm the pulldown of Flag-tagged PIWI proteins. Western blotting Tissues were

(12) Argonaute 2 inhibits RIG-I signaling via competition for viral RNA bindingiScienceSeptember 19, 2025Honglian Liu, Yingyin Liao, Fei Yu, Leo Ngo-Shing Li, Yajie Zhang, Lin Zhu, Guangshan Xie, Jiayan Liu, Siwen Liu, Shaofeng Deng, et al.rabbit a-TUBULIN (Abcam, ab18251), mouse b-ACTIN (Abcam, ab8226), rabbit-Flag (Thermo Fisher Scientific, PA1-984B), mouse-Flag (Sigma, M2/F1804), mouse-V5 (Bio-Rad, clone SV5-Pk1), rabbit-V5 (Cell Signaling Technology,

(13) A spectrum of cysteine mutations in fibulin-3 highlight its disulfide bonding complexity and potential to induce ER stressSci RepAugust 16, 2025Gracen E. Collier, Anna L. Roovers, John D. Hulleman(LI-COR). After washing in Tris-buffered saline supplemented with Tween-80 (0.5%, TBS-T), rabbit anti-flag (PA1-984B, 1:5,000, Pierce) and mouse anti-GAPDH (sc-47724, 1:10,000, Santa Cruz) were added to membranes for

(14) Exploration of a Class of Aryl Imidazolyl Ureas As Potent Acid Ceramidase Inhibitors for the Treatment of Fibrotic DiseasesACS OmegaAugust 12, 2025Richard Beresis, Vijaya Prathigudupu, Carson Cable, Amy Yu, Marc Adler, Roopa Ramamoorthi, Seul Ki Yeon, John D. Gordan, Sachin Sharma, William F. DeGrado, et al.Primary antibodies used were anti-YAP/TAZ (1:500, sc-101199, Santa Cruz) and anti-DYKDDDDK (1:500, PA1-984B, ThermoFisher Scientific). Cells were washed with PBS three times and incubated with Alexa 488 (1:400,

(15) GoLoco/GPR motif-dependent regulation of Rap1GAP1 by Gαo is disrupted by Gαo encephalopathy variantsJournal of Biological ChemistryAugust 1, 2025Nathalie L. Momplaisir, Naincy R. Chandan, Beiyun Wang, Elaine Qu, Alan V. SmrckaSignaling Technologies, D3H8Q), rabbit anti-HA (Cell Signaling Technologies, C29F4), Flag (Invitrogen, PA1-984B and Cell Signaling, 14793S), mouse anti-flag (Sigma-Aldrich, F1804), Rap1GAP1 (Proteintech, 19174-I-AP),

(16) SP140–RESIST pathway regulates interferon mRNA stability and antiviral immunityNatureJuly 31, 2025Kristen C. Witt, Adam Dziulko, Joohyun An, Filip Pekovic, Arthur Xiuyuan Cheng, Grace Y. Liu, Ophelia Vosshall Lee, David J. Turner, Azra Lari, Moritz M. Gaidt, et al.as previously described4; 1:1,000) and rabbit anti-Flag for Flag–TTP IPs (Thermo Fisher Scientific, PA1-984B, 1:1,000). In vivo L. pneumophila infections L. pneumophila infections were performed as described

(17) Integrating deep learning for post-translational modifications crosstalk on Hsp90 and drug bindingJ Biol ChemJuly 25, 2025Jennifer A. Heritz, Katherine A. Meluni, Sarah J. Backe, Sara J. Cayaban, Laura A. Wengert, Meik Kunz, Mark R. Woodford, Dimitra Bourboulia, Mehdi MollapourHsp90 monoclonal antibody (16F1) was purchased from Enzo. An anti-FLAG (DYKDDDDK) tag polyclonal antibody (PA1-984B) was purchased from ThermoFisher Scientific. Bacterial and viral strains DH5-alpha electrocompetent

(18) Citrate Transporter Expression and Localization: The Slc13a5Flag Mouse ModelInt J Mol SciJuly 12, 2025Jan C.-C. Hu, Tian Liang, Hong Zhang, Yuanyuan Hu, Yasuo Yamakoshi, Ryuji Yamamoto, Chuhua Zhang, Hui Li, Charles E. Smith, James P. Simmer8, Figure 9 and Figure 10). We used rabbit anti-DYKDDDDK recombinant protein polyclonal antibodies PA1-984B (Figure 7 and Figure 9) and 740001 (Figure 8 and Figure 10) from ThermoFisher Scientific to define the

(19) PIP4K2C inhibition reverses autophagic flux impairment induced by SARS-CoV-2Nat CommunJuly 10, 2025Marwah Karim, Manjari Mishra, Chieh-Wen Lo, Sirle Saul, Halise Busra Cagirici, Manon Gourdelier, Luca Ghita, Amrita Ojha, Do Hoang Nhu Tran, Aditi Agrawal, et al.PIKFYVE Polyclonal Antibody (Thermo Scientific, #PA5-13977), Flag (DYKDDDDK) Tag Polyclonal Antibody (Thermo Scientific, #PA1-984B). Immunofluorescence Antibodies targeting SARS-CoV-2 nucleocapsid (SinoBiological, #40143-MM05), EEA-1

(20) GADD45G operates as a pathological sensor orchestrating reactive gliosis and neurodegenerationNeuronJuly 9, 2025Tianjin Shen, Wenjiao Tai, Dongfang Jiang, Shuaipeng Ma, Xiaoling Zhong, Yuhua Zou, Chun-Li Zhang6E10 amyloid plaques (803007; mouse; 1:1000; Biolegend), FLAG (F1804; mouse; 1:1000; Sigma), FLAG (PA1–984B rabbit; 1:1000; Invitrogen), GFP (A-11122; rabbit; 1:1000; Invitrogen), MAP2 (MAP; chicken; 1:1000;

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DYKDDDDK Tag Antibody from Thermo Fisher Scientific

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DYKDDDDK Tag Antibody from Thermo Fisher Scientific

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