Western blot was performed using Anti-Rhodopsin Polyclonal Antibody (Product # PA1-729)and a 39 kDa band along with high molecular weight glycosylated forms corresponding to Rhodopsin were observed across all the tissues tested except Mouse Heart and Liver which are reported to be negative. Whole cell extracts (30 µg lysate) of Mouse Retina (Lane 1), Rat Retina (Lane 2), Rat Eye (Lane 3), Mouse Eye (Lane 4), Mouse Heart (Lane 5), and Mouse Liver (lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).