Western blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat (Lane 1), HEK 293 (Lane 2), Hep G2 (Lane 3), COLO 205 (Lane 4), MCF7 (Lane 5) and tissue extract of Mouse Colon (Lane 6). The blots were probed with Anti-ERp72 Rabbit Polyclonal Antibody (Product # PA1-007, 1:100-1:1000) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 72 kDa band corresponding to ERp72 was observed across the cell lines and tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).