Western blot analysis was performed on whole cell extracts (30 µg lysate) of Mouse Liver (Lane 1) and Rat Liver (Lane 2). The blots were probed with Anti-Cytochrome P450 3A4 Rabbit Polyclonal Antibody (Product # PA1-343, 1:1000-1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 57 kDa band corresponding to Cytochrome P450 3A4 was observed across tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).