RAT ANTI MOUSE CD206:Alexa Fluor® 488 MCA2235A488 from Bio-Rad (Formerly AbD Serotec)

Product Specs
ItemRAT ANTI MOUSE CD206:Alexa Fluor® 488
CompanyBio-Rad
Price $428.00Supplier Page View Company Product Page
Catalog NumberMCA2235A488
CloneMR5D3
NCBI Gene AliasesCD206, MR
TargetCD206
HostRat
Quantity100 TESTS/1ml
IsotypeIgG2a
ApplicationsFlow Cytometry
Conjugate/TagALEXA FLUOR® 488
ReactivityMouse
ClonalityMonoclonal Antibody
NCBI Full Gene Namemannose receptor, C type 1
ConcentrationIgG concentration 0.05 mg/ml

Questions and Answers

Can this antibody be used to identify macrophage populations in mouse tissues?

Yes, the RAT ANTI MOUSE CD206:Alexa Fluor® 488 antibody has been utilized in flow cytometry to stain mouse peritoneal macrophages, allowing for the identification of specific macrophage populations based on CD206 expression, as demonstrated in studies such as those by Eichin et al. (2015) and Han et al. (2020).

Is this antibody effective for studying macrophage polarization in mouse models?

The RAT ANTI MOUSE CD206 antibody has been shown to be effective in studies examining macrophage polarization. For instance, it was used in flow cytometry to analyze macrophage populations in the context of inflammation and immune responses, as reported in the research by Tuit et al. (2019), which correlates macrophage signatures with disease outcomes.

What is the significance of using this antibody in obesity-related inflammation studies?

This antibody can be particularly useful in obesity-related inflammation studies, as it helps identify adipose tissue macrophages, which play a crucial role in the inflammatory response associated with metabolic syndrome. The antibody's application in flow cytometry has been highlighted in research by Young et al. (2020), where it was used to assess immune cell populations in white adipose tissue.

Are there any specific experimental conditions recommended for using this antibody?

When using the RAT ANTI MOUSE CD206 antibody for flow cytometry, it is recommended to pre-block cells with Fc-block to reduce non-specific binding, as demonstrated in the methodologies of studies like those by Eichin et al. (2015) and Han et al. (2020). This step is crucial for obtaining accurate and reliable results in cell surface antigen analysis.

Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Macrophage-mediated refinement of the dural lymphatic regulates social behaviorNeuronApril 24, 2026Gabriel A. Tavares, Liam O'Reilly, Natalie M. Frederick, David A. Shapiro, Ashleigh Vicchiarelli, Megan Sullivan, Nathan Jiang, Maximillian Mattes, Coby Goelz, Nnadozie Uchegbu, et al.anti-DX5 (CD49b),BioLegend,"Cat# 108902, RRID:AB_313409" Rat monoclonal anti-CD206 A488,Bio-Rad,"Cat# MCA2235A488, RRID:AB_324891" Rat monoclonal anti-CD3e biotin,Thermo Fisher Scientific,"Cat# 13-0032-82, RRID:AB_2572762" Goat

(2) GHR^-/- Mice are protected from obesity-related white adipose tissue inflammationJournal of neuroendocrinologyNovember 1, 2020Young JA, Henry BE, Benencia F, Bell S, List EO, Kopchick JJ, Berryman DE.Target,Conjugate,Manufacturer,Catalog #,RRID CD206,AF488,BioRad,MCA2235A488,AB_324891 Ly-6C,PerCP-Cy5.5,Thermo Fisher,45–5932-80,AB_1518762 MHC-II,Biotin,Thermo Fisher,13–5321-81,AB_466661 F4/80,PE-Cy7,Thermo Fisher

(3) Regulation of adipose tissue inflammation by interleukin 6Proceedings of the National Academy of Sciences of the United States of AmericaFebruary 11, 2020Han MS, White A, Perry RJ, Camporez JP, Hidalgo J, Shulman GI, Davis RJ.antibodies including F4/80-Phycoerythrin (catalog no. 123110; Biolegend), CD206-Alexa Fluor 488 (catalog no. MCA2235A488; AbD serotec), and CD11c-Alexa Fluor 700 (catalog no. 56-0114-82; eBioscience) (33). Isotype control

(4) Transcriptional Signature Derived from Murine Tumor-Associated Macrophages Correlates with Poor Outcome in Breast Cancer PatientsCell reportsOctober 29, 2019Tuit S, Salvagno C, Kapellos TS, Hau CS, Seep L, Oestreich M, Klee K, de Visser KE, Ulas T, Schultze JL.AB_2566576 anti-mouse MHC-II,eBioscience,Cat. No. 47-5321-82; RRID: AB_1548783 anti-mouse CD206,AbD Serotec,Cat.No. MCA2235A488; RRID: AB_324891 anti-mouse CD3,eBioscience,Cat. No. 48-0031-82; RRID: AB_10735092 anti-mouse CD45,eBioscience,Cat.No.

(5) CD73 Activity is Dispensable for the Polarization of M2 MacrophagesPloS oneJanuary 1, 2015Eichin D, Laurila JP, Jalkanen S, Salmi M.following specific antibodies: anti-F4/80 FITC (rat IgG2b, #MCA497A488), anti-CD206 AF488 (rat IgG2a, #MCA2235A488) (both from AbD Serotec, Puchheim, Germany), anti-Ly6C FITC (rat IgG2c, #128006) and anti-F4/80 (rat

(6) Variable antigen uptake due to different expression of the macrophage mannose receptor by dendritic cells in various inbred mouse strainsImmunologyAugust 1, 2009Stella Eugenie Autenrieth, Ingo Birger Autenrieth

(7) Osteopontin promotes fibrosis in dystrophic mouse muscle by modulating immune cell subsets and intramuscular TGF-betaJournal of Clinical InvestigationJune 1, 2009Sylvia A. Vetrone, Encarnacion Montecino-Rodriguez, Elena Kudryashova, Irina Kramerova, Eric P. Hoffman, Scot D. Liu, M. Carrie Miceli, Melissa J. Spencer

(8) The potential of mannosylated chitosan microspheres to target macrophage mannose receptors in an adjuvant-delivery system for intranasal immunizationBiomaterialsDecember 15, 2007Hu-Lin Jiang, Mi Lan Kang, Ji-Shan Quan, Sang Gyun Kang, Toshihiro Akaike, Han Sang Yoo, Chong-Su Cho

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(1)

3/12/2019CD206 Antibody for ImmunofluorescenceDr. Efstathios StamatiadesGood antibody for direct staining of CD206 epitope.

RAT ANTI MOUSE CD206:Alexa Fluor® 488

RAT ANTI MOUSE CD206:Alexa Fluor® 488

RAT ANTI MOUSE CD206:Alexa Fluor® 488

RAT ANTI MOUSE CD206:Alexa Fluor® 488

RAT ANTI MOUSE CD206:Alexa Fluor® 488

RAT ANTI MOUSE CD206:Alexa Fluor® 488 from Bio-Rad (Formerly AbD Serotec)

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Questions and Answers

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Citations (8)

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RAT ANTI MOUSE CD206:Alexa Fluor® 488 from Bio-Rad (Formerly AbD Serotec)

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