RAT IgG2a NEGATIVE CONTROL antibody MCA1212 from Bio-Rad (AbD Serotec - FAB)

Product Specs
ItemRAT IgG2a NEGATIVE CONTROL antibody
CompanyBio-Rad
Price $127.00Supplier Page View Company Product Page
Catalog NumberMCA1212
Quantity1 ml
ApplicationsFlow Cytometry,ELISA
ConcentrationIgG concentration 0.1 mg/ml
FormatPurified IgG - liquid
IsotypeIgG2a
HostRat
ReactivityNegative Control
TypeNegative/Isotype Control

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Renal proximal tubular NEMO plays a critical role in ischemic acute kidney injuryJCI insightSeptember 17, 2020Han SJ, Williams RM, Kim M, Heller DA, D'Agati V, Schmidt-Supprian M, Lee HT.monoclonal antibody (14-5931-85, Thermo Fisher Scientific) as described (46, 53). Primary IgG2a antibody (MCA1212, AbD Serotec) was used as a negative isotype control. Quantification of kidney-infiltrating neutrophils

(2) Selective nanoparticle-mediated targeting of renal tubular Toll-like receptor 9 attenuates ischemic acute kidney injury.Kidney InternationalJuly 1, 2020Sang Jun Han, Ryan M. Williams, Vivette D’Agati, Edgar A. Jaimes, Daniel A. Heller, H. Thomas Leeanti-mouse F4/80 (Thermo Fisher Scientific, Inc., Pittsburgh, PA) as described52,53. Primary IgG2a antibody (MCA1212, AbD Serotec, Raleigh, NC) was utilized as a negative isotype control. Quantification of kidney infiltrating

(3) P2X4 receptor exacerbates ischemic AKI and induces renal proximal tubular NLRP3 inflammasome signalingThe FASEB JournalApril 1, 2020Sang Jun Han, Marianna Lovaszi, Mihwa Kim, Vivette D'Agati, György Haskó, H. Thomas Leeantibody (Thermo Fisher Scientific, Inc., Pittsburgh, PA) as described (32, 39). Primary IgG2a antibody (MCA1212, AbD Serotec, Raleigh, NC) was utilized as a negative isotype control (data not shown). Quantification

(4) P2X4 receptor exacerbates ischemic AKI and induces renal proximal tubular NLRP3 inflammasome signalingFASEB J.February 21, 2020Sang Jun Han, Marianna Lovaszi, Mihwa Kim, Vivette D’Agati, György Haskó, H. Thomas Leeantibody (Thermo Fisher Scientific, Inc., Pittsburgh, PA) as described (32, 39). Primary IgG2a antibody (MCA1212, AbD Serotec, Raleigh, NC) was utilized as a negative isotype control (data not shown). Quantification

(5) Peptidyl arginine deiminase-4 exacerbates ischemic AKI by finding NEMOAmerican Journal of Physiology-Renal PhysiologyJune 1, 2019May M. Rabadi, Sang Jun Han, Mihwa Kim, Vivette D’Agati, H. Thomas Leeagainst polymorphonuclear leukocytes (AbD Serotec, Raleigh, NC) and primary antibody recognizing IgG2a (MCA1212, AbD Serotec) as a negative isotype control in all experiments. Quantification of kidney infiltrating

(6) Kidney Proximal Tubular TLR9 Exacerbates Ischemic Acute Kidney InjuryThe Journal of ImmunologyAugust 1, 2018Sang Jun Han, Hongmei Li, Mihwa Kim, Mark J. Shlomchik, H. Thomas LeeLy6B monoclonal antibody (AbD Serotec, Raleigh, NC) as described (36, 44). Primary IgG2a antibody (MCA1212, AbD Serotec, Raleigh, NC) was utilized as a negative isotype control. Quantification of kidney infiltrating

(7) Divergent roles for kidney proximal tubule and granulocyte PAD4 in ischemic AKIAmerican Journal of Physiology-Renal PhysiologyMay 1, 2018Hongmei Li, Sang Jun Han, Mihwa Kim, Ahyeon Cho, Yewoon Choi, Vivette D’Agati, H. Thomas LeeLy6B monoclonal antibody (AbD Serotec, Raleigh, NC) as described (29, 30). Primary IgG2a antibody (MCA1212, AbD Serotec) was utilized as a negative isotype control. Quantification of kidney infiltrating neutrophils

(8) ATP induces PAD4 in renal proximal tubule cells via P2X7 receptor activation to exacerbate ischemic AKIAmerican Journal of Physiology-Renal PhysiologyFebruary 1, 2018May Rabadi, Mihwa Kim, Hongmei Li, Sang Jun Han, Yewoon Choi, Vivette D’Agati, H. Thomas LeeLy6B monoclonal antibody (AbD Serotec, Raleigh, NC) as described (43, 44). Primary IgG2a antibody (MCA1212, AbD Serotec) was utilized as a negative isotype control. Quantification of kidney infiltrating neutrophils

(9) Peptidyl arginine deiminase-4-deficient mice are protected against kidney and liver injury after renal ischemia and reperfusionAmerican journal of physiology. Renal physiologyAugust 1, 2016Rabadi M, Kim M, D'Agati V, Lee HT.monoclonal antibody (AbD Serotec, Raleigh, NC) as previously described (39, 40). Primary IgG2a antibody (MCA1212; AbD Serotec) was used as a negative isotype control. Neutrophils were quantified in five to seven randomly

(10) CNS-accessible Inhibitor of Glucosylceramide Synthase for Substrate Reduction Therapy of Neuronopathic Gaucher DiseaseMolecular TherapyJune 1, 2016John Marshall et al.

(11) Tasquinimod triggers an early change in the polarization of tumor associated macrophages in the tumor microenvironmentJournal for ImmunoTherapy of CancerDecember 15, 2015Anders Olsson et al.CD31 immunohistochemical staining was performed with rat antiCD31 monoclonal antibody (AbD Serotec, MCA1212) and peroxidase/diamminobenzidine revelation. Vascular density was evaluated for 3 regions of interest

(12) Critical role of interleukin-11 in isoflurane-mediated protection against ischemic acute kidney injury in miceAnesthesiologyDecember 1, 2013Ham A, Kim M, Kim JY, Brown KM, Yeh J, D'Agati VD, Lee HT.polymorphonuclear leukocytes (clone 7/4; AbD Serotec, Raleigh, NC). A primary antibody that recognized IgG2a (MCA1212; AbD Serotec) was used as a negative isotype control in all experiments. Neutrophils infiltrating the

(13) The volatile anesthetic isoflurane induces ecto-5′-nucleotidase (CD73) to protect against renal ischemia and reperfusion injuryKidney InternationalJuly 1, 2013Mihwa Kim, Ahrom Ham, Joo Yun Kim, Kevin M. Brown, Vivette D. D'Agati, H. Thomas Thomas Lee

(14) Endogenous control of immunity against infection: tenascin-C regulates TLR4-mediated inflammation via microRNA-155Cell reportsOctober 25, 2012Piccinini AM, Midwood KS.Immunostaining specificity was confirmed by omitting the primary antibody or using a nonimmune rat IgG2a (MCA1212; AbD Serotec). Neutrophil elastase+ cell number was determined by counting cells in five different areas

(15) Endogenous control of immunity against infection: tenascin-C regulates TLR4-mediated inflammation via microRNA-155Cell reportsOctober 25, 2012Piccinini AM, Midwood KS.Immunostaining specificity was confirmed by omitting the primary antibody or using a nonimmune rat IgG2a (MCA1212; AbD Serotec). Neutrophil elastase+ cell number was determined by counting cells in five different areas

(16) The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsisNature medicineAugust 1, 2012Schmidt EP, Yang Y, Janssen WJ, Gandjeva A, Perez MJ, Barthel L, Zemans RL, Bowman JC, Koyanagi DE, Yunt ZX, et al.for heparanase); goat IgG, AB-108-C, 1:1,000, R&D Systems (control for thrombomodulin); rat IgG2a, MCA1212, 1:300, AbD Serotec (control for Ly-6B.2). We performed image quantification using Metamorph as previously

(17) Isoflurane post-conditioning protects against intestinal ischemia-reperfusion injury and multiorgan dysfunction via transforming growth factor-β1 generationAnnals of surgeryMarch 1, 2012Kim M, Park SW, Kim M, D'Agati VD, Lee HT.antibody against PMN (clone 7/4, AbD Serotec, Raleigh, NC). A primary antibody that recognized IgG2a (MCA1212, AbD Serotec) was used as a negative isotype control in all experiments. Neutrophils per hpf were counted

(18) Old beagle dogs have lower faecal concentrations of some fermentation products and lower peripheral lymphocyte counts than young adult beaglesBritish Journal of NutritionOctober 19, 2011Márcia de Oliveira Sampaio Gomes, Mariana Casteleti Beraldo, Thaila Cristina Putarov, Márcio Antônio Brunetto, Leandro Zaine, Maria Beatriz Abreu Glória, Aulus Cavalieri Carciofi100 ml of blood were incubated with 2 ml of the antibodies (control isotypes y1/PE/R104 and y2/FITC/ MCA1212; CD5/FITC/MCA1037, CD4/PE/MCA1038, CD8/PE/ MCA1039, CD45/FITC/MCA1042F, CD21/PE/ MCA1781; Abd Serotec,

(19) Investigation of the regenerative capacity of an acellular porcine medial meniscus for tissue engineering applicationsTissue engineering. Part AJanuary 1, 2011Thomas W Stapleton, Joanne Ingram, John Fisher, Eileen Ingham

(20) Identification of macrosialin (CD68) on the surface of host macrophages as the receptor for the intercellular adhesive molecule (ICAM-L) of Leishmania amazonensisInternational Journal for ParasitologyJune 2, 2009Hang-Pong Ng, Sui-Chi Chiang, Yi Chi, Sho-Tone Lee