Western blot analysis was performed on Nuclear enriched cell extracts (30 µg lysate) of Jurkat (Lane 1), Hep G2 (Lane 2), Karpas 299 (Lane 3), HT-29 (Lane 4), THP-1 (Lane 5), SH-SY5Y (Lane 6), U-2 OS (Lane 7) and PC-3 (Lane 8). The blots were probed with Anti-FOXN3 Recombinant Rabbit Monoclonal Antibody (Product # 702555, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 54 kDa band corresponding to FOXN3 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).