Rabbit anti-Mouse IgM

ELISA of mouse immunoglobulins shows Rabbit Anti-Mouse IgM Secondary Antibody (Product # SA5-10214) reacts to mouse IgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of Rabbit Anti-Mouse IgM Secondary Antibody (Product # SA5-10214) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.