Fluorescent western blot was performed using Rabbit anti-Mouse IgG Fc Recombinant Secondary Antibody (Product # SA5-10211). Whole cell extracts of A-431 (Lane 1, 2, 3, 4), HaCaT (Lane 5, 6, 7, 8), SH-SY5Y (Lane 9), HeLa (Lane 10), MCF7 (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Cytokeratin 5 Monoclonal Antibody (Product # MA5-15347), and GAPDH Monoclonal Antibody (VI-01) (Product # MA5-15738). Secondary antibody (Product # A-10684, 1:5,000) was used for detection of Cytokeratin 5 and Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 790 (Product # A11369) was used a tertiary antibody. Images were captured in iBright™ FL 1500 (Product # A44115). Rabbit anti-Mouse IgG Fc Recombinant Secondary Antibody (Product # SA5-10211) specifically detects theCytokeratin 5 mouse primary antibody.