Western Blot was performed using Anti-Reelin Polyclonal Antibody (Product # PA5-65442) and a 400 kDa band corresponding to Reelin was observed across Hep G2 treated with PTI when compared to HCT 116. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with PTI (1X, for 4 hrs) (Lane 2), HCT 116 (Lane 3), HCT 116 treated with PTI (1X, for 4 hrs) (Lane 4) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Few uncharacterized bands were observed between 50-70 kDa.