RAT ANTI MOUSE MHC CLASS II I-A/I-E:FITC

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A lack of CD4⁺ T cells or of IFNγ increases MHC II⁺ cell infection. a. Naive mouse lungs were stained for AEC2 (SPC⁺), AM (CD11c⁺), and MHC II. The filled arrow shows an MHC II⁺ AM. White / red co-localization appears as pink. Most MHC II⁺ cells were AEC2 (open arrows). Red / green co-localization appears as yellow. Bars show mean ± SEM of counts for 3 fields of view per section, for 2 sections from each of 2 mice. b. Naïive and d5 infected lungs were stained for SPC, MHC II and viral antigens (MHV). In infected lungs, open arrows show MHC II⁺ AEC2, which are uninfected (MHV^-); white filled arrows show infected MHC II⁺ cells, which are SPC^-; the grey filled arrow shows a rare MHV⁺ AEC2 (colocalization appears as white), which is MHC II^-. Fig 5a shows that in naïve mice 70% of MHC II⁺ lung cells were SPC⁺. After infection, significantly fewer were SPC⁺ (p<0.001 by Student's 2 tailed unpaired t test), and negligible numbers of MHV⁺MHC II⁺ cells (<5%) were SPC⁺. Bars show mean ± SEM of counts for 3 fields of view per section, across 3 sections from each of 2 mice. c. Mice were depleted of CD8⁺ T cells (αC