Fig 2: IHC expressions by ImmunoRatio (ImageJ plugin software) processed from one patient with stage I, poor differentiated type gastric adenocarcinoma. Upper panel: the original hematoxylin image (total cell). Lower panel: “DAB” indicates labeled cells, a pseudo-colored image showing the segmented staining components. Labeling index is calculated by the percentage of DAB (positively stained cells) divided the total cell. Left: 94.8% by CK18, middle: 59.4% by Ki67, right: 25.8% by VDR.

Fig 3: IHC expressions (%) (Y axis) of VDR, Ki67, and CK18 (X axis) in areas of gastritis, IM, and cancer. The expression of each IHC stain in IM (orange bar) and cancer (gray bar) is compared with the expression in gastritis (blue bar). P-values are presented at the top of each comparison (Scheffe post-hoc analysis).