RAT ANTI MOUSE CD11b MCA74G from Bio-Rad (Formerly AbD Serotec)

Product Specs
ItemRAT ANTI MOUSE CD11b
CompanyBio-Rad
Price $408.00Supplier Page View Company Product Page
Catalog NumberMCA74G
NCBI Gene AliasesCD11b/CD18, CR3, CR3A, Cd11b, F730045J24Rik, Ly-40, MAC1, Mac-1, Mac-1a
ImmunogenT cell enriched splenocytes from B10 mice
TargetCD11b
CloneM1/70.15
FormatPurified IgG - liquid
ReactivityMouse, Human,Rabbit
IsotypeIgG2b
HostRat
Quantity0.25 mg
ClonalityMonoclonal Antibody
ApplicationsFCM, IF, IHC, IHC-p, IP
NCBI Full Gene Nameintegrin alpha M
ConcentrationIgG concentration 1.0 mg/ml

Questions and Answers

What applications has the RAT ANTI MOUSE CD11b antibody been used for in scientific research?

The RAT ANTI MOUSE CD11b antibody has been shown to work in various applications including flow cytometry, immunofluorescence, immunohistochemistry, immunohistochemistry on paraffin-embedded sections, and immunoprecipitation.

Can this antibody be used to study microglial activation in neuroinflammatory conditions?

Yes, the RAT ANTI MOUSE CD11b antibody is a microglia marker and has been utilized in studies investigating microglial activation in neuroinflammatory conditions, such as in the context of Parkinson's disease where it was used to assess microglial responses to α-synuclein aggregates.

Is the RAT ANTI MOUSE CD11b antibody effective in detecting changes in microglial glutamate release?

The antibody has been referenced in studies examining microglial glutamate release, particularly in the context of α-synuclein aggregates, indicating its utility in research focused on excitotoxicity and neuroinflammation.

What species can the RAT ANTI MOUSE CD11b antibody react with?

The antibody is reactive with mouse, human, and rabbit species, making it versatile for various experimental models in neuroscience research.

Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) cGAS-STING-NFκB PATHWAY PLAYS A ROLE IN BURN INJURY-INDUCED MUSCLE WASTINGShockSeptember 1, 2025Fei Xie, Zerong You, Bin Yan, Jiajia Dai, Jinsheng Yang, Shiyu Wang, Hiroki Ogata, Shingo Yasuhara, JA Jeevendra MartynF4/80 anti-mouse antibody (1: 400, sc-377009 AF488, Santa Cruz) and CD11b anti-mouse antibody (1: 400, MCA74G, BIO-RAD) for macrophage. Synaptophysin anti-mouse antibody (1: 300, ab32127, Abcam) was used for staining

(2) Microglia activation orchestrates CXCL10-mediated CD8(+) T cell recruitment to promote aging-related white matter degenerationNature neuroscienceJune 1, 2025Groh J, Feng R, Yuan X, Liu L, Klein D, Hutahaean G, Butz E, Wang Z, Steinbrecher L, Neher J, et al.appropriate combination of up to three of the following antibodies: rat anti-CD11b (Bio-Rad Laboratories, MCA74G; 1:100); hamster anti-CD11c (Thermo Fisher Scientific, MA11C5; 1:100); mouse antineurofilament H nonphosphorylated,

(3) Molecular pathology of acute spinal cord injury in middle-aged miceBIORXIVMay 14, 2025Corey Fehlberg, Danny John, Brian Kang, James S. Choi, Susana Cerqueira, Alexis Brake, Jae K. Leesealed using CoverGrip (Biotium; cat #23005). Primary antibodies used: rat anti-CD11b (1:200, BioRad, MCA74GA), chicken anti-GFAP (1:500, ab4674, Abcam), rabbit anti-PDGFRβ (1:200, ab32570, Abcam). Secondary antibodies

(4) Senescent cell reduction does not improve recovery in mice under experimental autoimmune encephalomyelitis (EAE) induced demyelinationJournal of neuroinflammationApril 7, 2025Manavi Z, Melchor GS, Bullard MR, Gross PS, Ray S, Gaur P, Baydyuk M, Huang JK.(eBioscience, 14–0032-85, 1:100), rat anti-B220 (Invitrogen, 14–0452-81, 1:100), rat anti-CD11b (Biorad MCA74G, 1:100), rabbit anti-Olig2 (Millipore Sigma, AB9610, 1:300), rabbit anti-Sox9 (EMD Millipore, AB5535,

(5) Senescent-like microglia limit remyelination through the senescence associated secretory phenotypeNature communicationsMarch 7, 2025Gross PS, Durán-Laforet V, Ho LT, Melchor GS, Zia S, Manavi Z, Barclay WE, Lee SH, Shults N, Selva S, et al.(CC1) (Abcam #ab16794, 1:100), goat anti-CCL11 (R&D Systems #AF-420-SP, 1:50), rat anti-Cd11b (Biorad #MCA74G, 1:100), CD68 (Biolegend #137020, 1:100), rat anti-Clec7a (InvivoGen #mabg-mdect, 1:100), mouse anti-GFAP

(6) Microglial cell proliferation is regulated, in part, by reactive astrocyte ETBR signaling after ischemic strokeExperimental NeurologyMarch 1, 2025John J. McInnis, Matthew D. LeComte, Leah F. Reed, Emily E. Torsney, Roxana del Rio-Guerra, Matthew E. Poynter, Jeffrey L. Speesab16667, Abcam), and CD68 (1:200, MCA1957GA, BioRad), NG2 (1:200, MAB5384; Millipore), CD11b (1:200, MCA74G, Bio-rad), and CD45 (1:1000, CBL1326; Chemicon), TMEM119 (1 μg/mL, ab209064, Abcam), and Tie2 (1:100,

(7) Astrocytic heterogeneous nuclear ribonucleoprotein U is involved in scar formation after spinal cord injuryJournal of neuroinflammationJanuary 31, 2025Quan L, Uyeda A, Manabe I, Muramatsu R.(1:1000, 550278; BD Pharmingen), rabbit anti-Iba1 (1:1000, 019-19741, Wako), rat anti-CD11b (1:1000, MCA74GA, BioRad), rabbit anti-PDGFRβ (1:500, ab32570; Abcam), rabbit anti-5-HT (1:1000, 20080; Immunostar),

(8) Blood-derived APLP1+ extracellular vesicles are potential biomarkers for the early diagnosis of brain diseasesSci AdvJanuary 3, 2025Yuri Choi, Jae Hyun Park, Ala Jo, Chul-Woo Lim, Ji-Min Park, Jin Woo Hwang, Kang Soo Lee, Young-Sang Kim, Hakho Lee, Jisook MoonBiotechnology, catalog no. sc-514360), mouse anti-CD11b (1:25 dilution; AbD Serotec, NC, USA, catalog no. MCA74G; RRID: AB_321293), mouse anti-GLAST (1:100 dilution; Merck Millipore, catalog no. MABN794; RRID: AB_2811303). Enrichment

(9) C5aR1 antagonism suppresses inflammatory glial responses and alters cellular signaling in an Alzheimer's disease mouse modelNature communicationsAugust 15, 2024Schartz ND, Liang HY, Carvalho K, Chu SH, Mendoza-Arvilla A, Petrisko TJ, Gomez-Arboledas A, Mortazavi A, Tenner AJ.at 4 °C with agitation: rabbit anti-Iba1 (1:1000, Wako #019-19741), rat anti-CD11b (1:1000, Biorad #MCA74G), hamster anti-CD11c (1:400, Biorad #MCA1369), goat anti-Lcn2 (1:50, R&D #AF1857), chicken anti-GFAP

(10) Delayed plasma kallikrein inhibition fosters post-stroke recovery by reducing thrombo-inflammationJournal of NeuroinflammationJune 13, 2024Steffen Haupeltshofer, Stine Mencl, Rebecca D. Szepanowski, Christina Hansmann, Ana I. Casas, Hanna Abberger, Wiebke Hansen, Alina Blusch, Cornelius Deuschl, Michael Forsting, et al.(ab207327, Abcam, 1:200), anti-bradykinin-1 receptor (B1R; ABR-011, Alomone Labs, 1:200), anti-CD11b (MCA74G, Biorad, 1:200, unfixed tissue) and anti-NG2 (ab5320, Millipore, 1:200). All secondary antibodies were

(11) Parkinson's disease-derived α-synuclein assemblies combined with chronic-type inflammatory cues promote a neurotoxic microglial phenotypeJournal of neuroinflammationFebruary 21, 2024Yildirim-Balatan C, Fenyi A, Besnault P, Gomez L, Sepulveda-Diaz JE, Michel PP, Melki R, Hunot S.The following antibodies were used: anti-mouse CD25 (Bio-Rad, MCA1260), anti-mouse CD11b (Bio-Rad, MCA74G), anti-human CCR2 (R&D Systems; MAB150-100), anti-human CX3CR1 (Bio-Rad, AHP566). Mouse TNFα ELISA Kit

(12) Myeloid cell-associated aromatic amino acid metabolism facilitates CNS myelin regenerationNPJ Regenerative medicineJanuary 2, 2024Hu J, Melchor GS, Ladakis D, Reger J, Kim HW, Chamberlain KA, Shults NV, Oft HC, Smith VN, Rosko LM, et al.(eBioscience, #14-0032-85, 1:100), rat anti-B220 (Invitrogen, #14-0452-81, 1:100), rat anti-CD11b (BioRad, #MCA74G, 1:100), rabbit anti-Sox9 (EMD Millipore, #AB5535, 1:1500), rat anti-CD68 (BioLegend, #137020, 1:100),

(13) C5aR1 antagonism suppresses inflammatory glial gene expression and alters cellular signaling in an aggressive Alzheimer's modelbioRxiv : the preprint server for biologyAugust 22, 2023Schartz ND, Liang HY, Carvalho K, Chu SH, Mendoza-Arvilla A, Petrisko TJ, Gomez-Arboledas A, Mortazavi A, Tenner AJ.Primary antibodies used were rabbit anti-Iba1 (1:1000, Wako #019-19741), rat anti-CD11b (1:1000, Biorad #MCA74G), hamster anti-CD11c (1:400, Biorad #MCA1369), goat anti-Lcn2 (1:50, R&D #AF1857), chicken anti-GFAP

(14) Cytotoxic CNS-associated T cells drive axon degeneration by targeting perturbed oligodendrocytes in PLP1 mutant miceiScienceMay 19, 2023Abdelwahab T, Stadler D, Knöpper K, Arampatzi P, Saliba AE, Kastenmüller W, Martini R, Groh J.BioLegend), rat anti-Ly6A/E FITC (1:100, catalog no. 108105; BioLegend), rat anti-CD11b (1:100, catalog no. MCA74G; Bio-Rad Laboratories), rabbit anti-CD11b (1:100, catalog no. NB110-89474; Novus Biologicals), rat anti-Sn

(15) Inflammasome inhibition protects dopaminergic neurons from α-synuclein pathology in a model of progressive Parkinson’s diseaseJournal of NeuroinflammationMarch 21, 2023Alexander Grotemeyer, Judith F. Fischer, James B. Koprich, Jonathan M. Brotchie, Robert Blum, Jens Volkmann, Chi Wang Ip(1:100, AdipoGen, cat. #AG-20B-0014-C; RRID:AB_2885199), rat anti-mouse CD11b (1:300, Bio-Rad, cat. #MCA74G; RRID:AB_321293), rat anti-mouse CD8 (1:500, Bio-Rad, cat. #MCA609G; RRID:AB_321407), or rat anti-mouse CD4

(16) C5aR1 antagonism alters microglial polarization and mitigates disease progression in a mouse model of Alzheimer’s diseaseActa Neuropathologica CommunicationsAugust 17, 2022Angela Gomez-Arboledas et al.#019–19,741), rat monoclonal anti-CD68 (1:700 Biolegend #137,001), rat monoclonal anti-CD11b (Biorad #MCA74G), Armenian hamster monoclonal anti-CD11c (1:400, Biorad #MCA1369), rat monoclonal anti-C3 (1:50, Hycult

(17) Boosting the peripheral immune response in the skeletal muscles improved motor function in ALS transgenic miceMolecular therapy : the journal of the American Society of Gene TherapyAugust 3, 2022Trolese MC, Scarpa C, Melfi V, Fabbrizio P, Sironi F, Rossi M, Bendotti C, Nardo G.anti-neurofilament heavy polypeptide (NF200, 1:1,000; N4142, Sigma-Aldrich); rat anti-CD11b (1:200; MCA74G, BioRad); rabbit anti-iNOS (1:200; PA3-030A, Invitrogen); rabbit anti-mannose receptor (CD206, 1:200;

(18) Modulation of C5a-C5aR1 signaling alters the dynamics of AD progressionJournal of neuroinflammationJuly 11, 2022Carvalho K, Schartz ND, Balderrama-Gutierrez G, Liang HY, Chu SH, Selvan P, Gomez-Arboledas A, Petrisko TJ, Fonseca MI, Mortazavi A, et al.(1:1000, Wako #019-19741), rat anti-CD68 (1:700, Biolegend #137001), rat anti-CD11b (1:100, Biorad #MCA74G), hamster anti-CD11c (1:400, Biorad #MCA1369), rat anti-Lamp1 (1:500, Abcam #ab25245), rat anti-mouse

(19) Myelin and non-myelin debris contribute to foamy macrophage formation after spinal cord injuryNeurobiology of diseaseFebruary 1, 2022Ryan CB, Choi JS, Al-Ali H, Lee JK.blocking buffer (5% NGS in PBS-T). Antibodies used include rat anti-mouse CD11b (1:200, Biorad Ref MCA74GA), chicken anti-mouse GFAP (1:500, Abcam, ab4674), and biotinylated mouse-NeuN antibody (1:400, Millipore,

(20) Single-cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordThe Journal of experimental medicineAugust 2, 2021Milich LM, Choi JS, Ryan C, Cerqueira SR, Benavides S, Yahn SL, Tsoulfas P, Lee JK.ab183597/AB-2832971; 1:100), CD63 (PE/Dazzle 594; Biolegend; 143913/2565503; 1:100), and rat anti-CD11b (BioRad; MCA74GA/AB-324660; 1:500). In situ hybridization Tissue sections were prepared as described above. In situ hybridization

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RAT ANTI MOUSE CD11b

RAT ANTI MOUSE CD11b

RAT ANTI MOUSE CD11b

RAT ANTI MOUSE CD11b

RAT ANTI MOUSE CD11b

RAT ANTI MOUSE CD11b from Bio-Rad (Formerly AbD Serotec)

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RAT ANTI MOUSE CD11b from Bio-Rad (Formerly AbD Serotec)

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