Western blot analysis was performed on whole cell extracts (30 µglysate) of HeLa (Lane 1), HeLa treated with IFN gamma (100 ng/mL IFN gamma for 72h) (Lane 2), U-937 (Lane 3), Raji (Lane 4) and Ramos (Lane 5).The blots were probed with PSMB8 Rabbit polyclonal Antibody (Product # PA1-972, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 20 kDa band corresponding to PSMB9 was observed across the cell line tested and was enhanced upon IFN gamma treatment in HeLa cell line. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).