EOMES Antibody AF6166 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemEOMES Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF6166
NCBI Gene AliasesTBR2
Conjugate/TagUnconjugated
ReactivityGuinea Pig,Human,Mouse,Primate - Pan troglodytes (Chimpanzee),Primate - Pongo (Orangutan),Rabbit,Transgenic Mouse
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Intracellular Staining by Flow Cytometry,Immunocytochemistry,CyTOF-ready
TargetEOMES
HostSheep
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
Quantity100 ug (also 25 ug)
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human EOMES, Gly471-Pro686, Accession # O95936
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Nameeomesodermin

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human EOMES in Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study EOMES expression in human neural progenitor cells?

Yes, the EOMES antibody has been utilized in various studies, including those examining its role in neural progenitor cells. For instance, it was used at a dilution of 1:500 in immunohistochemistry to investigate the expression of EOMES in human cortical tissues, highlighting its relevance in neurogenesis and cortical development ().

What is the recommended dilution for using this antibody in immunocytochemistry?

The EOMES antibody is recommended to be used at a dilution of 1:500 for immunocytochemistry, as demonstrated in studies focusing on neural progenitor cells and their differentiation ().

Is this antibody effective for detecting EOMES in mouse tissues as well?

Yes, the EOMES antibody has shown reactivity with both human and mouse tissues, making it suitable for studies involving EOMES in various species, including mouse models of neurodevelopmental disorders ().

Are there any specific applications where this antibody has been cited in the literature?

The EOMES antibody has been cited in multiple applications, including Western blotting, flow cytometry, immunocytochemistry, and immunohistochemistry, indicating its versatility in studying EOMES across different experimental setups (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Multiomics analysis identifies VPA-induced changes in neural progenitor cells, ventricular-like regions, and cellular microenvironment in dorsal forebrain organoidsMolecular PsychiatryApril 24, 2026Zeynep Yentür, Lizia Branco, Kseniia Sarieva, Daria Andreeva, Theresa Kagermeier, Christina Kulka, Mohamed A. Jarboui, Federico Colombo, Florencia Diaz, Pierre Collignon, et al.9661S) anti-PAX6 (mouse, 1:500, HPA, Cat. no. AMAb91372), anti-TBR2 (sheep, 1:500, Biotechne, Cat. no. AF6166), anti-COL IV (rabbit, 1:50, Abcam, Cat. no. ab6586)) were washed three times with PBS for 15 min. Secondary

(2) Electrophysiological development and functional plasticity in dissociated human cerebral organoids across multiple cell linesCell Reports MethodsApril 20, 2026Adam Pavlinek, Sara Guerrisi, Kara O'Driscoll, Lucia Dutan Polit, Roland Nagy, APEX consortium, Madeline A. Lancaster, Anthony C. Vernon, Deepak P. Srivastavaanti-TBR1 (1:200),Abcam,Cat# ab31940; RRID: AB_2200219 Sheep anti-EOMES (TBR2) (1:200),Biotechne,Cat# AF6166; RRID: AB_10569705 Mouse anti-DLX2 (1:200),Santa Cruz,Cat# SC-393879; RRID: AB_3678657 Rabbit anti-NEUROD2

(3) Multi-omic analysis of guided and unguided forebrain organoids reveals differences in cellular composition and metabolic profilesCell Reports MethodsFebruary 23, 2026Marie Sejberg Øhlenschlæger, Pia Jensen, Jesper Foged Havelund, Sissel Ida Schmidt, Fadumo Abdullahi Mohamed, Magdalena Sutcliffe, Sofie Blomberg Elmkvist, Lucrezia Criscuolo, Steven W. Wingett, Ilaria Chiaradia, et al.polyclonal anti-SOX2,Abcam,"Cat# ab97959, RRID: AB_2341193" Sheep polyclonal anti-TBR2,R&D Systems,"Cat# AF6166, RRID: AB_10569705" Mouse monoclonal anti-HuC/D,Thermo Fisher Scientific,"Cat# A-21271, RRID: AB_221448" Mouse

(4) Dmrt2 regulates sex-biased neuronal development in the cingulate cortexCell Mol Life SciOctober 30, 2025Ana Bermejo-Santos, Miguel Rubio-García, Rodrigo Torrillas-de la Cal, Rafael Casado-Navarro, Esther Serrano-Saizanti-CTIP2 1:500 (ab18465, Abcam), rabbit anti-Ki67 1:1000 (ab15580, Abcam) and sheep anti-TBR2 1:200 (AF6166, R&D System). In the case of TBR2, heat-induced antigen retrieval was performed before primary antibody

(5) A post-implantation model of human embryo development includes a definitive hematopoietic nicheCell reportsOctober 28, 2025Neupane J, Jowett GM, Wu B, Verma A, Dietmann S, Reid AJ, Surani MA.

(6) Single-cell analysis of dup15q syndrome reveals developmental and postnatal molecular changes in autismNat CommunJuly 4, 2025Yonatan Perez, Dmitry Velmeshev, Li Wang, Matthew L. White, Clara Siebert, Jennifer Baltazar, Guolong Zuo, Juan Andrés Moriano, Songcang Chen, David M. Steffen, et al.species,Manufacturer,Identifier,Dilution MBP,goat,Santa cruz bio,SC-13914,1:500 SOX2,Mouse,Santa cruz bio,sc-365823,1:500 EOMES,Sheep,R&D systems,AF6166,1:500 BCL11B,Rat,Abcam,ab18465,1:500 HOPX,Rabbit,Proteintech,11419-1-AP,1:500 SATB2,mouse,Abcam,ab51502,1:500 NeuN,Guinea pig

(7) Dysregulation of mTOR signalling is a converging mechanism in lissencephalyNatureJanuary 1, 2025Ce Zhang, Dan Liang, A. Gulhan Ercan-Sencicek, Aybike S. Bulut, Joelly Cortes, Iris Q. Cheng, Octavian Henegariu, Sayoko Nishimura, Xinyuan Wang, A. Buket Peksen, et al.BD Biosciences); rabbit anti-EOMES (TBR2) (1:500; HPA028896, Sigma); sheep anti-EOMES (TBR2) (1:200; AF6166, R&D Systems); and rabbit anti-phospho-S6 ribosomal protein (pS6) (1:200; 4858, Cell Signaling Technologies).

(8) The characteristics of excitatory lineage differentiation and the developmental conservation in Reeler neocortexCell proliferationMay 1, 2024Deng HH, Tong SY, Shen D, Zhang SQ, Fu Y.the primary antibodies used were chicken anti‐GFP (Aves lab, GFP‐1020), Sheep anti‐Tbr2 (R&D system, AF6166), Rat anti‐Tbr2 (Thermo Fisher Scientific, 14487582, 1:1000), Mouse anti‐IdU (Sigma‐Aldrich, SAB3701448),

(9) Cerebral organoids display dynamic clonal growth and tunable tissue replenishmentNature cell biologyMay 1, 2024Lindenhofer D, Haendeler S, Esk C, Littleboy JB, Brunet Avalos C, Naas J, Pflug FG, van de Ven EGP, Reumann D, Baffet AD, et al.

(10) Thalamocortical organoids enable in vitro modeling of 22q11.2 microdeletion associated with neuropsychiatric disordersCell Stem CellMarch 1, 2024David Shin, Chang N. Kim, Jayden Ross, Kelsey M. Hennick, Sih-Rong Wu, Neha Paranjape, Rachel Leonard, Jerrick C. Wang, Matthew G. Keefe, Bryan J. Pavlovic, et al.1:300)",Biolegend,901301 "PAX6 (sheep, 1:500)",R&D Systems,AF8150 "EOMES (sheep, 1:300)",R&D Systems,AF6166 "EOMES (mouse IgG1k, 1:500)",eBioscience,14-4877-82 "TBR1 (rabbit, 1:200)",Abcam,ab31940 "SATB2 (mouse,

(11) Joint epigenome profiling reveals cell-type-specific gene regulatory programmes in human cortical organoidsNature cell biologyDecember 1, 2023Noack F, Vangelisti S, Ditzer N, Chong F, Albert M, Bonev B.

(12) Reduced neural progenitor cell count and cortical neurogenesis in guinea pigs congenitally infected with Toxoplasma gondiiCommunications BiologyNovember 27, 2023Thomas Grochow, Britta Beck, Zaida Rentería-Solís, Gereon Schares, Pavlo Maksimov, Christina Strube, Lisa Raqué, Johannes Kacza, Arwid Daugschies, Simone A. FietzBiolegend, London, United Kingdom, 901301), Tbr2 (1:200, sheep, R&D Systems, Abingdon, United Kingdom, AF6166), Hu C/D (1:500, rabbit, Abcam, Amsterdam, Netherlands, ab184267), neurofilament H (1:500, chicken,

(13) Neocortex neurogenesis and maturation in the African greater cane ratNeural DevelopmentOctober 13, 2023Oluwaseun Mustapha, Thomas Grochow, James Olopade, Simone A. Fietzrabbit, Biozol, Eching, Germany, BLD-901301), Tbr2 (1:100, sheep, R&D Systems, Abingdon, United Kingdom, AF6166), Tbr1 (1:200, rabbit, Millipore, Darmstadt, Germany, AB10554), MAP2 (1:100, chicken, Abcam, Amsterdam,

(14) LIF signaling regulates outer radial glial to interneuron fate during human cortical developmentCell Stem CellOctober 5, 2023Madeline G. Andrews, Clara Siebert, Li Wang, Matthew L. White, Jayden Ross, Raul Morales, Megan Donnay, Gradi Bamfonga, Tanzila Mukhtar, Arpana Arjun McKinney, et al.AB_2064130 "anti-DLX5, rabbit, 1:100",Sigma,Cat#HPA005670 RRID:AB_1078681 "anti-EOMES, sheep, 1:250",R&D Systems,Cat#AF6166 RRID: AB_10569705 "anti-GFAP, goat, 1:500",Abcam,Cat#ab4674 RRID:AB_304558 "anti-GFP, chicken, 1:500",Aves labs

(15) Tissue morphology influences the temporal program of human brain organoid developmentCell stem cellOctober 5, 2023Chiaradia I, Imaz-Rosshandler I, Nilges BS, Boulanger J, Pellegrini L, Das R, Kashikar ND, Lancaster MA.

(16) Single cell analysis of dup15q syndrome reveals developmental and postnatal molecular changes in autismbioRxiv : the preprint server for biologySeptember 22, 2023Perez Y, Velmeshev D, Wang L, White M, Siebert C, Baltazar J, Dutton NG, Wang S, Haeussler M, Chamberlain S, et al.species,Manufacturer,Identifier,Dilution MBP,goat,Santa cruz bio,SC-13914,1:500 SOX2,Mouse,Santa cruz bio,sc-365823,1:500 EOMES,Sheep,R&D systems,AF6166,1:500 BCL11B,Rat,Abcam,ab18465,1:500 HOPX,Rabbit,Proteintech,11419-1-AP,1:500 SATB2,mouse,Abcam,ab51502,1:500 NeuN,Guinea pig

(17) Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoidseLifeMarch 29, 2023Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, Dürnberger G, Mechtler K, Knoblich JA.

(18) SARS-CoV-2 infects human brain organoids causing cell death and loss of synapses that can be rescued by treatment with SofosbuvirPLoS biologyNovember 1, 2022Mesci P, de Souza JS, Martin-Sancho L, Macia A, Saleh A, Yin X, Snethlage C, Adams JW, Avansini SH, Herai RH, et al.

(19) Androgens increase excitatory neurogenic potential in human brain organoidsNatureFebruary 1, 2022Kelava I, Chiaradia I, Pellegrini L, Kalinka AT, Lancaster MA.(Abcam, ab110123, 1:200), goat anti-BRN2 (Santa Cruz, sc-6029, 1:200), sheep anti-TBR2 (R&D Systems, AF6166, 1:200). The next day, sections were washed with PBS and secondary antibodies coupled to Alexa Fluor

(20) Endosomal trafficking defects alter neural progenitor proliferation and cause microcephalyNature communicationsJanuary 10, 2022Carpentieri JA, Di Cicco A, Lampic M, Andreau D, Del Maestro L, El Marjou F, Coquand L, Bahi-Buisson N, Brault JB, Baffet AD.(Abcam ab18465, 1/300), rabbit anti Pax6 (Biolegend 901301 1/500), Sheep anti TBR2/EOMES (R&D system AF6166 1/500), rabbit anti NEUN (Abcam ab177487 1/1000), goat anti Phospho Histone3 (Santa Cruz SC-12927 1/1000),

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Human EOMES Antibody

EOMES Antibody

EOMES Antibody

EOMES Antibody

EOMES Antibody

EOMES Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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EOMES Antibody from R&D Systems, a Bio-Techne Brand

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