Fig 1: Involvement of ATM/p53 signaling pathway in PLB-induced G2/M cell cycle arrest. (A,B) HCC cells were pretreated with NAC (10 mM), KU-55933 (10 μM) and Pifithrin-α (Pftα,10 μM) or not for 1 h, and 10 μM of PLB was added into the medium for 12 h. Cell percentage at different cell cycle stages was analyzed based on the flow cytometry results. (C,D) The expression of cdc25C, p-p53 (Ser15) and p21 was determined by Western blot analysis. (E,F) Gray value statistical analysis of the indicated proteins. * p < 0.05, ** p < 0.01 compared to the DMSO group, # p < 0.05, ## p < 0.01 compared to the PLB treatment group; three independent experiments were performed.
Fig 2: Plumbagin-induced ROS-dependent cdc25C downregulation. (A,B) HCC cells were pretreated with NAC (10 mM) or not for 1 h, and 10 μM of PLB was added into the medium for 12 h. The expression of cdc25C and p-cdc25C (Ser216) was determined by Western blot analysis. (C,D) Gray value statistical analysis of the indicated proteins. (E,F) Cells were treated as mentioned above and the relative mRNA levels of gene CDC25C were determined by the RT-qPCR method. ** p < 0.01 vs. compared to the DMSO group, ## p < 0.01 compared to the PLB group; three independent experiments were performed.
from Cell Signaling Technology for cdc25C Antibody Sampler Kit