Western blot was performed using UQCRC2 Polyclonal Antibody (Product # PA5-53939) and a 48 kDa band corresponding to UQCRC2 was observed in Hep G2, HEK-293, A-431, U-2 OS and Mouse Heart and Mouse Kidney. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (Lane 2), A-431 (Lane 3) and U-2 OS (Lane 4), Mouse Heart (Lane 5) and Mouse Kidney (Lane 6) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).