Immunohistochemical staining of DUS3L in human cerebral cortex tissue shows strong cytoplasmic positivity in neuronal cells. Samples were probed using a DUS3L Polyclonal Antibody (Product # PA5-59757).

Western blot analysis of DUS3L in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4. Samples were probed using a DUS3L Polyclonal Antibody (Product # PA5-59757).

Immunoprecipitation of DUS3L was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of polyclonal DUS3L antibody (Product # PA5-59757) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti-rabbit coated Dynabeads (Product # 11204D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.