Immunofluorescent staining of SPT5 in human cell line A-431 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a SPT5 Polyclonal Antibody (Product # PA5-56100).

Western blot was performed using Anti-SPT5 Polyclonal Antibody (Product # PA5-56100) and a 150 kDa band corresponding to SPT5 was observed across all the cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), NIH/3T3 (Lane 3) and K-562 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by XCell SureLock™ Mini-Cell and XCell II™ Blot Module (Product # EI0002). The blot was probed with the primary antibody (0.4 ug/ml) and detected by chemiluminescence with Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).