Immunofluorescent analysis of HA Tag was performed using HA-H3 construct transfected in HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HA Tag Polyclonal Antibody, eBioscience™ (Product # 14-67-5663) at 1 µg/mL and Histone H3 Monoclonal Antibody (865R2) Product # AHO1432) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555 (Product # A32732) and Goat anti-Mouse IgM (Heavy Chain) Secondary Antibody, Alexa Fluor 488 (Product # A-21042) respectively at a dilution of 1:2,000 for 45 minutes at room temperature. Panel a (Nuclei: Red) represents HA Tag Polyclonal Antibody. Panel b (Nuclei: Green) represents Histone H3. Panel c (Nuclei: Blue) represents ProLongTM Diamond Antifade Mountant with DAPI (Product # P36962). Panel d represents the merged image showing the co-localization of nuclear signals in transfected cells. Panel e represents untransfected HEK cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Immunofluorescent analysis of HA Tag was performed using HA-H3-FLAG construct transfected in HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HA Tag Polyclonal Antibody, eBioscience™ (Product # 14-6756-81, 14-6756-63) at 1 µg/mL and Histone H3 Monoclonal Antibody (865R2) Product # AHO1432) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555 (Product # A32732) and Goat anti-Mouse IgM (Heavy Chain) Secondary Antibody, Alexa Fluor 488 (Product # A-21042) respectively at a dilution of 1:2000 for 45 minutes at room temperature. Panel a (Nuclei: Red) represents HA Tag Polyclonal Antibody. Panel b (Nuclei: Green) represents Histone H3. Panel c (Nuclei: Blue) represents ProLongTM Diamond Antifade Mountant with DAPI (Product # P36962). Panel d represents the merged image showing the co-localization of nuclear signals in transfected cells. Panel e represents untransfected HEK cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supplier Page from Thermo Fisher Scientific for HA Tag Antibody