Fig 1: Specificity and broad dynamic range of peptide arrays.(a) Images displaying reactive peptide features from an array probed with HA-tag antibody (eBioscience 14-6756-81). Amino acid sequence of peptide targets is displayed in parentheses. Residues corresponding to HA-tag sequence (NH2-YPYDVPDYA-COOH) are underlined in seasonal H3N2 peptide sequences. (b) Graph displaying MFI of array peptide features depicted in a in a titration experiment using HA-tag antibody ranging in concentration from 2000 ng mL-1 to 0 ng mL-1. Error bars in b reflect mean ± SEM of triplicate array feature MFI for each titration point.
Fig 2: Peptide arrays allow for discrimination of unique peptide antibody targets.(a) Images displaying reactive HA-tag, seasonal H3N2 H3–4-7 and FLAG-tag peptide features on arrays probed with HA-tag antibody (14-6756-81, eBioscience) or FLAG-tag antibody (A9594, Sigma) either not pre-cleared, or after three rounds of clearing with HA-tag peptide or FLAG-tag peptide conjugated to streptavidin beads. (b) Histogram displaying percent (%) of maximum MFI for peptide features shown in a. Error bars in b represent the mean ± SEM of % of maximum MFI of triplicate array features. For each peptide, % of maximum MFI of the highest MFI replicate feature (of three) in the non-pre-cleared condition was calculated.
Supplier Page from Thermo Fisher Scientific for HA Tag Antibody