Immunohistochemistry analysis of BAZ1A in human testis and pancreas tissues. Samples were incubated with BAZ1A polyclonal antibody (Product # PA5-51736). Corresponding BAZ1A RNA-seq data are presented for the same tissues.
Immunohistochemical staining of BAZ1A in human testis tissue shows strong nuclear positivity in cells of seminiferus ducts. Samples were probed using a BAZ1A Polyclonal Antibody (Product # PA5-51736).
Immunoprecipitation of BAZ1A was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of polyclonal BAZ1A antibody (Product # PA5-51736) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti-rabbit coated Dynabeads (Product # 11204D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.
Supplier Page from Thermo Fisher Scientific for BAZ1A Antibody