Fig 2: Representative FACS plots for gating strategy for sorting of human MuSCs and FAPs following full staining(P1) FSC-A vs SCC-A plot to collect cells of the right size.(P2) FSC-A vs. FSC-H plot to collect single cells only.(P3) CD31/45-FITCH vs. SSC-A plot to exclude endothelial cells and immune cells through negative selection.(P4) CD56-Brilliant Violet vs. CD34-APC plot to distinguish 34+ cells (red box) and 34- cells (blue box).(P5) CD90-PE vs. CD34-APC plot to distinguish between the CD90+ cells (red box) and CD90- cells (black box) of the CD34+ cells from (P4). These are the FAPs.(P6) CD56-Brilliant Violet vs. CD82-PE-Vio770 plot to collect the CD56+CD82+ cells from the CD34- population of P4. These are the MuSCs (blue box).

Fig 3: Representative FACS plots for FMO control for CD34-APC(P1) FSC-A vs SCC-A plot to collect cells of the right size.(P2) FSC-A vs. FSC-H plot to collect single cells only.(P3) CD31/45-FITCH vs. SSC-A plot to exclude endothelial cells and immune cells through negative selection.(P4) CD56-Brilliant-Violet vs. CD34-APC plot to distinguish 34+ and 34- cells. As this is the FMO CD34-APC there are no CD34+ cells (red box).

Fig 4: Representative FACS plots for FMO control for CD90-PE(P1) FSC-A vs SCC-A plot to collect cells of the right size.(P2) FSC-A vs. FSC-H plot to collect single cells only.(P3) CD31/45-FITCH vs. SSC-A plot to exclude endothelial cells and immune cells through negative selection.(P4) CD56-Brilliant-Violet vs. CD34-APC plot to distinguish 34+ cells (red box) and 34- cells (blue box).(P5) FMO CD90-PE vs. CD34-APC plot to distinguish between the CD90+ and CD90- populations of the 34+ cells from (P4). As this is the FMO CD90-PE control there are no CD90+ cells (black box).

Fig 5: Representative FACS plots for FMO control for CD82-PE-Vio770(P1) FSC-A vs SCC-A plot to collect cells of the right size.(P2) FSC-A vs. FSC-H plot to collect single cells only.(P3) CD31/45-FITCH vs. SSC-A plot to exclude endothelial cells and immune cells through negative selection.(P4) CD56-Brilliant Violet vs. CD34-APC plot to distinguish 34+ cells (red box) and 34- cells (blue box).(P5) CD34-APC vs. FMO CD82-PE-Vio770 plot to distinguish between the CD82+ cells and CD90- cells of the CD34- cells from (P4). As this is the FMO CD82-PE-Vio770 there are no CD82+ cells (blue box).