PE anti-mouse CD3 100206 from BioLegend

Product Specs
ItemPE anti-mouse CD3
CompanyBioLegend
Price $143.00Supplier Page View Company Product Page
Catalog Number100206
NCBI Gene AliasesCtg-3, Ctg3, T3g
HostRat
Conjugate/TagPE
ReactivityMouse
Quantity200 µg
ApplicationsFC - Quality tested
Clone17A2
IsotypeRat IgG2b, kappa
Antibody TypePrimary
NCBI Full Gene NameCD3 antigen, gamma polypeptide
Concentration0.2mg/ml
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenγδTCR-positive T-T hybridoma D1
ClonalityMonoclonal
TargetCD3

Questions and Answers

Can this antibody be used for flow cytometry in mouse models?

Yes, the PE anti-mouse CD3 antibody has been shown to be effective for flow cytometry applications, as evidenced by its use in various studies, including those that analyze immune cell populations in mouse models ().

What specific immune cells can I expect to detect using this antibody?

The PE anti-mouse CD3 antibody targets CD3G, which is a component of the T cell receptor complex. This allows for the identification and analysis of T cells, including both CD4+ and CD8+ T cell populations in mouse samples ().

Is there any evidence of this antibody being used in studies related to immune responses?

Yes, the antibody has been utilized in research examining immune responses, such as in the study of the effects of Yu-Ping-Feng on T cell populations and macrophage polarization in tumor microenvironments, highlighting its relevance in immunological research ().

What is the host species for this antibody, and how does that affect its use in experiments?

The PE anti-mouse CD3 antibody is derived from rat, which is important for ensuring compatibility in experiments involving mouse models. This host species selection helps to minimize cross-reactivity and enhances the specificity of the antibody for mouse CD3G ().

BioLegend

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San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) mRNA lipid-nanoparticle-mediated mitochondrial apoptosis augments adoptive T cell immunotherapyCell Reports MedicineApril 21, 2026Jiayan Fu, Yaoqi Liu, Zhenyu Zhong, Benyuan Cao, Luna Ran, Zijia Guo, Haiyang Dong, Nengcheng Bao, Rongqing Pan, Jinqiang Wang, et al.reagents. APC Anti-mouse CD45 (BioLegend, catalog no. 147708), PE Anti-mouse CD3 (BioLegend, catalog no. 100206) were employed to label the cell surface. The cells were labeled for 30 min at 4°C, protected from light.

(2) Short-chain acyl-CoA dehydrogenase initiates mtDNA demethylation and leakage to fuel antitumor immunity in colorectal cancerSignal Transduct Target TherMarch 26, 2026Fang Yang, Meng Wang, Shaofan Hu, Xu Guan, Kun Zhao, Yong Zhou, Hui Yao, Tianying Zhang, Liuli Li, Yuan Gao, et al.BioLegend); Alexa Fluor 700 anti-mouse CD45 antibody (#103128, BioLegend), PE anti-mouse CD3 antibody (# 100206, BioLegend), APC/Cy7 anti-mouse CD4 antibody (#116020, BioLegend), Percp5.5 anti-mouse CD8a (#100734,

(3) A single-dose of PDGFB circular RNA enables sustained growth factor expression to accelerate diabetic wound healingJournal of NanobiotechnologyFebruary 7, 2026Yi-Qi Shen, Yan Zhang, Liu-Yi Yao, Ru-Ke Zhang, Bin Yang, Cheng-Cheng Deng(AF3628, 1:2000; R&D Systems; stock concentration 0.2 mg/ml), Collagen I (ab270993, 1:2000; Abcam), CD3 (100206, 1:2000; BioLegend), CD20 (PA516701, 1:600; Thermo), CD68 (ab955, 1:50; Abcam), TNFα (26405-1-AP, 1:5000;

(4) Fatty acid uptake mediated by FABP4 promotes the formation of CD8+T cell senescence through lipid peroxidation in the adipocyte-rich microenvironment of Ovarian CancerOncogenesisFebruary 6, 2026Chunyan Yu, Xin Li, Xiaolong Qian, Haoke Zhang, Xueying Li, Bo Wang, Mantong Li, Zixuan Liu, Wei Du, Siqi Chen, et al.(0.25 μl, Invitrogen; Cat# 45-0451-82, RRID: AB_1107002), anti-mouse PE-CD3 (0.25 μl, Biolegend; Cat# 100206, RRID: AB_312663), anti-mouse PE/Cyanine7-CD3 (0.2 μl, Biolegend; Cat# 100220, RRID: AB_1732057), anti-mouse

(5) RP-182 alleviated obstruction-induced renal fibrosis by reprogramming CD206+ macrophagesFrontiers in PharmacologyJanuary 30, 2026Hualin Cao, Xiaoye Chen, Ruyue Jin, Yunjie Yang, Yuandong Tao, Pin Li, Yangyang Wu, Guilong Chen, Jiawen Zhao, Jianhua Wen, et al.Ly6G-APC/Cyanine7 (127624), Ly6C-PE (128008), F4/80-APC (123116), CD206-PE/Cyanine7 (141720), CD3-PE (100206), CD4-PE/Cyanine7 (116016), CD8a-APC/Cyanine7 (100713), NK1.1-FITC (156508), and CD20-APC (152107).

(6) MyD88 signaling in myeloid cells induces gastrointestinal tract injury and systemic inflammation after West Nile virus infectionCell ReportsJanuary 27, 2026Shih-Ching Lin, Fang R. Zhao, Matthias Mack, Gabriel Mbalaviele, Michael S. Diamondantibody,BioLegend,Cat#109820; RRID:AB_492872 PE-conjugated anti-mouse CD3 antibody,BioLegend,Cat#100206; RRID:AB_312663 PE-conjugated anti-mouse CD19 antibody,BioLegend,Cat#115508; RRID:AB_313643 AF700-conjugated

(7) Engineered ETS1‐Nanoconjugate Restores Immune Homeostasis through Dual Immune‐Vascular Modulation in Relapsing and Progressive Multiple SclerosisAdvanced Healthcare MaterialsJanuary 27, 2026Feng Zhang, Si‐Bo Yang, Yan Luo, Xiao‐Di Sun, Bo‐Hao Chang, Da‐Qiang Zhou, Jun‐Jie Jiang, Ya‐Nan Li, Jie‐Hong Wu, Hui‐Juan Jin, et al.with an antibody cocktail containing CD45‐FITC (1:100, BioLegend #103108), CD3‐PE (1:100, BioLegend #100206), CD4‐BB700 (1:100, BD #566407), CD8a‐APC‐Cy7 (1:100, BioLegend #100713), CD31‐BV421 (1:100, BioLegend

(8) Glutamine-driven upregulation of NPDC1 promotes colorectal cancer progression through PI3K/AKT signalingJournal of Translational MedicineJanuary 24, 2026Qing Qin, Dapeng Zhang, Yusai Xie, Fan Zhang, Yulan Huang, Renchao Deng, Minli Yang, Lili Zhang, Houjie Liang, Rongchen ShiAlexa Fluor 700 anti-mouse CD45 antibody (#103128, BioLegend), phycoerythrin (PE) anti-mouse CD3 antibody (#100206, BioLegend), Peridinin Chlorophyll Protein (PerCP/Cy5.5) anti-mouse CD8a antibody (#100734, BioLegend),

(9) Selective Immune Silencing by Targeted TGF-β AgonistsbioRxivJanuary 23, 2026Qinli Sun, Masato Ogishi, Hua Jiang, Alison K. Barrett, Hao Yan, Elsa Sola, Jie Zhang, Peng Xiao, Huiyun Lyu, Ahmad Salehi, et al.guidelines. Flow cytometry The following antibodies were purchased from BioLegend: mouse CD45.2 (109839), CD3 (100206), CD4 (100453, 100430, 100428), CD8 (100706), NK1.1 (156506), TCRvα2 (127806, 127822), Thy1.1 (202528,

(10) An innovative engineered IL-10 monomer strengthens T cell-mediated anti-tumor responses through anti-PD-1 cis-deliveryCell Reports MedicineJanuary 20, 2026Ce Gu, Jian Guo, Chang Zhou, Peipei Hu, Xiaodong Wu, Jiaojiao Ding, Xinxin Zhou, Liao Zeng, Wen Yu, Yingye Ou, et al.GHI/61),BioLegend,Cat#: 333606; PRID: AB_1134002 PE anti-mouse/human CD3 Antibody (clone: 17A2),BioLegend,Cat#: 100206; PRID: AB_312663 PE anti-mouse/human CD44 Antibody (clone: IM7),BioLegend,Cat#: 103007; PRID: AB_312958 FITC

(11) A genetically encoded reporter reveals interferon responses in multiple cell lineagesbioRxivJanuary 13, 2026Sarah R. Anderson, Pailin Chiaranunt, Nicholas M. Mroz, Sarah D. Wade, Ari B. Molofsky, Anna V. MolofskyBiotec,130-117-386 CD31–PE-vio770,390,1:100,Milltenyi Biotec,130-102-375 CD206–BV605,C068C2,1:200,Biolegend,141721 CD3–PE,17A2,1:200,Biolegend,100206 CD31–BV421,390,1:400,Biolegend,102424 Sca-1–BV711,D7,1:200,Biolegend,108131 CD8α–BV785,53-6.7,1:200,Biolegend,100750 PDGFRα–APC,APA5,1:200,Biolegend,135908 EpCAM–APC-Fire750,G8.8,1:200,Biolegend,118230 CD4–BV711,RM4-5,1:200,Biolegend,100557 TCRγδ–PerCP-Cy5.5,GL3,1:200,Biolegend,118118 Ly-6C–BV605,HK1.4,1:400,Biolegend,128035 NK1.1–BV650,PK136,1:200,Biolegend,108736 CD64–AF647,X54-5/7.1,1:200,BD Biosciences

(12) Boron neutron capture therapy preserves immune cells and induces robust anti-tumour immunity in preclinical mouse modelNature CommunicationsJanuary 8, 2026Qi Sun, Yanping Zhao, Simiao Qiao, Kexuan Wang, Chuanjie Lu, Zizhu Zhang, Zhibin Guo, Zexuan Ding, Chunhong Wang, Jiyuan Li, et al.and BMDCs at 8:1. After 72 h incubation, T cells were analysed after staining with anti-mouse CD3-PE (100206, BioLegend), anti-mouse CD25-PE/Cyanine 7 (102016, BioLegend), and anti-mouse CD69-BV421 (104545, BioLegend)

(13) CCL19-armed recombinant influenza virus inhibited colorectal cancer growth by remodeling tumor micro-environmentiScienceDecember 19, 2025Xia Ou, Yunxin Xia, Zhongyue Fang, Kai Yang, Guangtao Yang, Junying Wang, Yiyong Duan, Xiahui Yang, Bing Yang, Ze Liu, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, FITC anti-mouse CD45,Biolegend,103108 PE anti-mouse CD3,Biolegend,100206 APC anti-mouse CD8a,Biolegend,100712 PerCP anti-mouse CD4,Biolegend,100538 PE anti-mouse CD25,Biolegend,102008 APC anti-mouse F4/80

(14) Transcriptional dynamics of CD8+ T-cell exhaustion in immune checkpoint inhibitor resistance at single-cell resolutionMol CancerDecember 11, 2025Tzu-Yang Tseng, Ching-Hung Hsieh, Hsuan-Cheng Huang, Yu-Ching Wu, Chiun Hsu, Chia-Lang Hsu, Da-Liang Ou, Hsueh-Fen JuanBioLegend: CD3-FITC (BioLegend Cat# 155604), CD3-APC (BioLegend Cat# 100236), CD3-PE (BioLegend Cat# 100206), CD8-APC (BioLegend Cat# 100712), CD8-APC-Cy7 (BioLegend Cat# 100714), CD45-BV605 (BioLegend Cat# 103140),

(15) Silencing PCSK9 reshapes the spatiotemporal activation of STING for safe and effective cancer immunotherapyNature CommunicationsNovember 25, 2025Pengbo Sun, Fangping Han, Xinyan Li, Chengcheng Wu, Tingyue Deng, Jia He, Conggang Zhang, Rui KuaiPE anti-mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5, Biolegend, Cat# 108408), PE anti-mouse CD3 (17A2, Biolegend, Cat# 100206) and PerCP anti-mouse CD25 (PC61, Biolegend, Cat# 102028) at 4 °C for 1 h. For intracellular marker

(16) A Novel Self-Regulated, Non-Directional Magnetic Thermos-Brachytherapy (125)I Seed Enhances Anticancer Efficacy by Rescuing Immune EscapeAdvanced science (Weinheim, Baden-Wurttemberg, Germany)November 1, 2025Xu B, Wu YM, Cheng YF, Dong L, Ding SW, Chen SR, Chen BD, Yang RP, Zhang WH, Wang YJ, et al.BioLegend), Csf3r (APC, orb1676847, Biorbyt), Cd274 (PE, 558091, BD), Cd3 (FITC, 100203, BioLegend; PE, 100206, BioLegend), Pd‐1 (PE, 135215, BioLegend), Ifn‐γ (BV786, 563773, BD), Cd68 (APC, 137008, BioLegend),

(17) Light Metabolically Reprograms CD8 + T Cells to Potentiate STING‐Driven Tumor Eradication and Prevent MetastasisAdvanced ScienceOctober 23, 2025Asmita Banstola, Shilin Gao, Zhengkung Zhang, Yan Dong, Prabhat Upadhyay, Quanwei Zhang, Yongli Li, Zuan‐Tao Lin, Zhilong Wang, Mei X. Wufluorescein isothiocyanate (FITC) CD3 (Clone: 17A2; catalog no: 100204), PE CD3 (clone: 17A2; catalog no: 100206), Pacific Blue CD8a (clone: 53‐6.7; catalog no: 100725), PE NK‐1.1 (clone: S17016D; catalog no: 156504),

(18) 15-PGDH inhibition enhances hematopoietic regeneration during agingStem CellsSeptember 17, 2025Rahul Chaudhary, Brittany A. Cordova, Marcus Hong, Bailey R. Klein, Lyannah A. Contreras, Ritisha Rashmil, Filip Goshevski, Julianne N.P. Smith, Derek J. Taylor, Andrew A. Pieper, et al.quantify the populations below: HSPCs: Cells were stained with antibodies to: CD3 (Biolegend, Catalog # 100206), Ly-6G/Ly-6C (Invitrogen, Catalog # 12-5931-82), B220 (Biolegend, Catalog # 103208), Ter119 (BD Biosciences,

(19) CARG-2020 targets IL-12, IL-17, and PD-L1 pathways to effectively treat melanoma and breast cancerSci RepAugust 13, 2025Elham Ahmadi, Conner Chiari, Bhaskara Reddy Madina, Timur Olegovich Yarovinsky, Marie Marthe Krady, Ju Chen, Bijan Almassian, Valerian Nakaar, Kepeng Wangfluorochrome-conjugated antibodies in PBS with 2% fetal bovine serum (FBS) and 1mM EDTA. Anti-CD3 (Cat # 100206), anti-CD4 (Cat # 100536), anti-CD45 (Cat # 103138), anti-NK1.1 (Cat # 156503), anti-IFN-γ (Cat # 505808)

(20) Deep metabolic profiling of immune cells by spectral flow cytometry—A comprehensive validation approachiScienceJuly 18, 2025Claire L. Wishart, Alanna G. Spiteri, Jian Tan, Claudio Counoupas, James A. Triccas, Laurence Macia, Nicholas J.C. King123123 Anti-CD3e (clone 500A2) – AF700,BD,Cat # 557984 Anti-CD3e (clone 17A2) – PE,Biolegend,Cat # 100206 Anti-Siglec-F (clone E50-2440) – BV480,BD,Cat # 746668 Anti-Siglec-F (clone RP/14) – PE,BD,Cat # 552128 Anti-CD103

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12/1/2015CD3 PE Antibody Review Michaela RekowSplenocytes from latently infected mice are analyzed for various immune cells (T and B cells) and those are quantified at various time points of latent infection.

PE anti-mouse CD3 from BioLegend

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PE anti-mouse CD3 from BioLegend

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