Calbindin D28k Antibody 214 004 from Synaptic Systems GmbH

Product Specs
ItemCalbindin D28k Antibody
CompanySynaptic Systems GmbH
Price $400.00Supplier Page View Company Product Page
Catalog Number214 004
Formatlyophilized
Quantity100 µl
HostGuinea pig
ApplicationsWB, IP, ICC, IHC, IHC-P
Reactivityhuman, rat, mouse, monkey, ape, cow
TargetCalbindin D28k
Clonalitypolyclonal
ImmunogenRecombinant protein corresponding to AA 3 to 251 from human CalbindinD28k
Antibody TypePrimary antibody

Questions and Answers

Can this Calbindin D28k antibody be used for immunohistochemistry in mouse models?

Yes, the Calbindin D28k antibody has been successfully utilized in immunohistochemistry for mouse models, as demonstrated in studies where it was used to identify Purkinje neurons in cerebellar tissue sections (; ).

What is the recommended dilution for using this antibody in immunofluorescence applications?

The recommended dilution for the Calbindin D28k antibody in immunofluorescence applications is typically 1:500 to 1:1000, as indicated in various studies that employed this antibody for visualizing Purkinje cells and other neuronal types (; ).

Is this antibody effective for detecting Calbindin D28k in human tissues?

While the primary focus of the Calbindin D28k antibody has been on rodent models, it has also been referenced in studies involving human brain samples, suggesting its potential effectiveness in detecting Calbindin D28k in human tissues ().

What specific applications has this antibody been shown to work in?

The Calbindin D28k antibody has been shown to work in various applications including Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation, making it versatile for different experimental setups (; ).

Synaptic Systems GmbH

Rudolf-Wissell-Str. 28

Goettingen 37079

Germany

Phone: +49-551-50556-0
+49-551-50556-384

Company Profile

Website: http://www.sysy.com/

(1) Impaired dynein function preserves spinal interneuron survival and positioning in an ALS-like mouse modelPLoS OneApril 2, 2026Eleni Christoforidou, Jordan S. Rowe, Fabio A. Simoes, Raphaelle Cassel, Luc Dupuis, Peter Nigel Leigh, Majid HafezparastMerck/Sigma, AB144P), polyclonal guinea pig anti-calbindin D28k (1:200 dilution, Synaptic Systems, 214 004), polyclonal rabbit anti-parvalbumin (1:500 dilution, Synaptic Systems, 195 002), and polyclonal chicken

(2) Cerebellar climbing fibers impact experience-dependent plasticity in the mouse primary somatosensory cortexeLifeDecember 22, 2025Abby Silbaugh, Kevin P Koster, Christian Hanseldonkey serum and anti-Calbindin (Guinea pig polyclonal) primary antibody (1:500; Synaptic Systems Cat# 214 004, RRID:AB_10550535; Synaptic Systems GmbH, Germany). After washing the tissue three times for 15 min

(3) AAV-based gene therapy ameliorates neurological deficits in a mouse model of childhood-onset neurodegeneration with cerebellar atrophyMolecular TherapyNovember 5, 2025Hiroko Wada, Noriyuki Hama, Koichi Hasegawa, Chie Takamuku, Mieko Yoshida, Mika Omura, Asuka Araki, Ken-ichiro Kuwakobuffer. The following primary antibodies were used: anti-HA (3F10, Roche, 1:2,000), anti-calbindin (214 004, Synaptic Systems, 1:1,000), anti-GFP (GFP-1010, Aves, 1:1,000), and anti-calbindin (CB955, Sigma-Aldrich,

(4) Convergence and Segregation of Excitatory and Inhibitory Afferents in the Paraventricular Thalamic NucleusJ NeurosciOctober 22, 2025László Biró, Zsolt Buday, Kata Kóta, Szabolcs Lőrincz, László Acsády,Synaptic Systems,AB_2156476 PV,Mouse,"1:1,000",P3088,Sigma-Aldrich,AB_477329 5-HT,Goat,"1:8,000",20079,ImmunoStar,AB_572262 ChAT,Rabbit,"1:2,000",297-013,Synaptic Systems,AB_2620040 CB,Guinea pig,"1:2,000",214-004,Synaptic Systems,AB_10550535 Overview of primary antibodies used in this study

(5) Non-allometric expansion and enhanced compartmentalization of Purkinje cell dendrites in the human cerebellumElifeApril 15, 2025Silas E Busch, Christian Hanselfollowed by incubation in guinea pig anti-calbindin primary antibody (1:1000; Synaptic Systems Cat# 214 004, RRID:AB_10550535) solution overnight (18–20 hr) at 4°C with 1% NDS in PBS-TX. After 3×10 min washes

(6) The Burning Pain Transcriptome in the Mouse Primary Somatosensory CortexInt J Mol SciApril 9, 2025Virág Erdei, Zoltán Mészár, Angelika VargaUSA",NBP2-58646 calbindin D28k,guinea pig polyclonal IgG,1:250,"Synaptic Systems GmbH.; Göttingen, Germany",214004 calretinin,guinea pig polyclonal IgG,1:500,"Swant; Burgdorf, Switzerland",CRGp7 parvalbumin,mouse

(7) Ankyrins are dispensable for mechanotransduction by cochlear hair cellsHearing ResearchApril 1, 2025Hong Zhang, Xufeng Qiu, Jonah Mittelstadt, Ulrich Mülleranti-MYO6 (1:500, Proteus Biosciences, Cat# 25–6791), guinea pig anti-CALB1 (1:500, Synaptic Systems, Cat# 214004), mouse anti-ANK1 (1:100, Antibodies Incorporated, Cat# 75–380), guinea pig anti-ANK3 (1:500, Synaptic

(8) Non-allometric expansion and enhanced compartmentalization of Purkinje cell dendrites in the human cerebellumbioRxivNovember 2, 2024Silas E. Busch, Christian Hanselfollowed by incubation in guinea pig anti-calbindin primary antibody (1:1000; Synaptic Systems Cat# 214 004, RRID:AB_10550535) solution overnight (18–20hrs) at 4°C with 1% normal donkey serum in PBS-TX. After

(9) Non-canonical function of ADAM10 in presynaptic plasticityCellular and molecular life sciences : CMLSAugust 9, 2024Bär J, Fanutza T, Reimann CC, Seipold L, Grohe M, Bolter JR, Delfs F, Bucher M, Gee CE, Schweizer M, et al.Enzo",Clone SAP7F407;Cat#ADI-VAM-PS003;RRID:AB_10618753 Anti-calbindin (guinea pig),Synaptic Systems,Cat#214004;RRID:AB_10550535 Anti-calretinin (mouse),Synaptic Systems,Cat#214111;RRID:AB_2619904 Anti-GFP (mouse),Biolegends,Cat#902601;RRID:AB_2565021 Anti-MAP2

(10) Selective induction of human renal interstitial progenitor-like cell lineages from iPSCs reveals development of mesangial and EPO-producing cellsCell reportsFebruary 27, 2024Tsujimoto H, Hoshina A, Mae SI, Araoka T, Changting W, Ijiri Y, Nakajima-Koyama M, Sakurai S, Okita K, Mizuta K, et al.

(11) Endosomal dysfunction contributes to cerebellar deficits in spinocerebellar ataxia type 6eLifeDecember 12, 2023Cook AA, Leung TCS, Rice M, Nachman M, Zadigue-Dube É, Watt AJ.(goat),R&D Systems,AF6617,1:500,3 Calbindin,Anti-calbindin polyclonal (guinea pig),Synaptic Systems,214 004,1:500,"2, 3, 6, 9" Rab7,Anti-Rab7 monoclonal (mouse),Cell Signaling Technology,95746,1:400,4 Calbindin,Anti-calbindin

(12) Decreasing mutant ATXN1 nuclear localization improves a spectrum of SCA1-like phenotypes and brain region transcriptomic profilesNeuronFebruary 15, 2023Handler HP, Duvick L, Mitchell JS, Cvetanovic M, Reighard M, Soles A, Mather KB, Rainwater O, Serres S, Nichols-Meade T, et al.

(13) Overexpression of the autism candidate gene Cyfip1 pathologically enhances olivo-cerebellar signaling in miceFrontiers in cellular neuroscienceJanuary 1, 2023Busch SE, Simmons DH, Gama E, Du X, Longo F, Gomez CM, Klann E, Hansel C.anti-NRXN1 (1:200; Millipore; Cat# MABN607) and guinea pig anti-calbindin (1:1000; Synaptic Systems; Cat# 214 004; RRID:AB_10550535). After 3 × 10 min washes in PBS-TX at RT, the secondary antibody incubation lasted

(14) Role of GluA4 in the acoustic and tactile startle responsesHearing ResearchFebruary 1, 2022Sofía García-Hernández, María E. Rubiorabbit anti-GluA4 (2 μg/mL; Chemicon AB1508) or guinea pig anti-Calbindin (1:500; Synaptic Systems 214 004), were added to the sections and incubated overnight at 4°C in blocking buffer. After rinsing well with

(15) Experimental Epileptogenesis in a Cell Culture Model of Primary Neurons from Rat Brain: A Temporal Multi-Scale StudyCellsNovember 3, 2021Jablonski J, Hoffmann L, Blümcke I, Fejtová A, Uebe S, Ekici AB, Gnatkovsky V, Kobow K.

(16) Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survivalCell reportsSeptember 14, 2021Lowden C, Boulet A, Boehler NA, Seecharran S, Rios Garcia J, Lowe NJ, Liu J, Ong JLK, Wang W, Ma L, et al.

(17) The Bacterial Enzyme RfxCas13d Is Less Neurotoxic Than PspCas13b and Could Be a Promising RNA Editing and Interference Tool in the Nervous SystemBrain sciencesAugust 9, 2021Wu QW, Kapfhammer JP.

(18) Total Number and Ratio of GABAergic Neuron Types in the Mouse Lateral and Basal AmygdalaThe Journal of neuroscience : the official journal of the Society for NeuroscienceMay 26, 2021Vereczki VK, Müller K, Krizsán É, Máté Z, Fekete Z, Rovira-Esteban L, Veres JM, Erdélyi F, Hájos N.

(19) Murine Esophagus Expresses Glial-Derived Central Nervous System AntigensInternational Journal of Molecular SciencesMarch 22, 2021Christopher Kapitza, Rittika Chunder, Anja Scheller, Katherine S. Given, Wendy B. Macklin, Michael Enders, Stefanie Kuerten, Winfried L. Neuhuber, Jürgen WörlSpecies,Dilution,Source (Catalogue Number) Calbindin D28k,Guinea pig,1:100,"Synaptic SystemsGöttingen, Germany (214 004)" ChAT,Goat,1:40,"MilliporeTemecula, CA, USA (AB144P–1ML)" DsRed,Rabbit,1:1000,"Takara BioMountain

(20) Single cell RNA sequencing uncovers cellular developmental sequences and novel potential intercellular communications in embryonic kidneyScientific reportsJanuary 8, 2021Matsui I, Matsumoto A, Inoue K, Katsuma Y, Yasuda S, Shimada K, Sakaguchi Y, Mizui M, Kaimori JY, Takabatake Y, et al.Medical & Biological Laboratories, Nagoya, Japan), collagen I (ab34710, Abcam), and calbindin D28k (214004, Synaptic Systems, Goettingen, Germany). For the detection of myosin-11, α-smooth muscle actin, claudin

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Calbindin D28k Antibody

Calbindin D28k Antibody

Calbindin D28k Antibody

Calbindin D28k Antibody

Calbindin D28k Antibody from Synaptic Systems GmbH

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Synaptic Systems GmbH

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Calbindin D28k Antibody from Synaptic Systems GmbH

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