Western blot analysis of Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 ug per lane using HICE
Supplier Page from Biorbyt for HICE1 antibody
Application Notes: Hice1 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kDa in size corresponding to human Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Application Dilutions: ELISA: 1:30,000-1:45,000, WB: 0.45 µg/mL