Fig 1: PRP-1 effect on TLR receptors and adaptor proteins in human chon-drosarcoma JJ012 cell line. PRP-1 upregulated protein expression of TLR1 and TLR2 in dose-dependent manner in human JJ012 chondrosarcoma cell lysates. TLR3 expression was very weak and the data is not shown. TLR4, TLR5 proteins were expressed but PRP-1 did not have any effect. Tubulin was used as loading control. The bands were detected for TLR1, TLR2, TLR4 and TLR5 and tubulin at 120, 119, 130, 90 and 50 kDa, correspondingly. PRP-1 upregulated protein expression of TLR6 in dose-dependent manner in human JJ012 chondrosarcoma cell lysates. TLR7 was not expressed in this cell line at all. TLR8, 9 and 10 were expressed but no effect of PRP-1 was observed. Tubulin was used as housekeeping control. The bands were detected for TLR6, TLR8, TLR9, TLR10 and tubulin at 100, 86, 100, 120 and 50 kDa, correspondingly. PRP-1 upregulated TICAM2 (TRAM) adaptor protein in dose-response manner but did not have any effect on TICAM1 (TRIF) adaptor protein in human JJ012 chondrosarcoma cell line. Tubulin was used as loading control. The bands were detected for TICAM1, TICAM2 and tubulin.