OctA-Probe (H-5) Antibody sc-166355 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemOctA-Probe (H-5) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-166355
Antibody TypePrimary Antibodies
Quantity200 µg/ml
CloneH-5
ApplicationsWB, IP, IF, ELISA
ReactivityOctA tagged proteins, FLAG tagged proteins
Conjugate/TagUnconjugated
FormatPurified
Hostmouse
ClonalityMonoclonal
IsotypeIgG1
TargetOctA-Probe

Questions and Answers

Can this antibody be used for immunoprecipitation in my experiments?

Yes, the OctA-Probe (H-5) antibody has been shown to be effective for immunoprecipitation, as demonstrated in various studies including those that utilized it for protein interaction assays (see and ).

What applications has this antibody been successfully used in?

The OctA-Probe (H-5) antibody has been successfully utilized in several applications including Western Blotting, ELISA, Immunofluorescence, and Immunoprecipitation, as indicated by its use in multiple scientific publications (cited in 95 articles).

Is this antibody suitable for detecting FLAG-tagged proteins in mouse models?

Yes, the OctA-Probe (H-5) antibody is specifically designed to recognize FLAG-tagged proteins and has been utilized in mouse models for various studies, including those examining protein interactions and signaling pathways (referenced in and ).

What is the host species for this antibody, and does it react with other species?

The OctA-Probe (H-5) antibody is derived from mouse and is primarily reactive with the FLAG epitope tag. It is not specified to react with other species, so its use is primarily focused on systems expressing FLAG-tagged proteins.

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) miRNA‑378a‑5p attenuates the development of abdominal aortic aneurysm via ABLIM1‑MKL1 signaling pathwaysInternational Journal of Molecular MedicineFebruary 16, 2026Jing Wang, Yujia Zou, Yani Wang, Zheming Yang, Daoshen Liu, Xiaolin Su, Haixu Song, Kai Xu, Chenghui Yan, Dan Liu, et al.Fisher Scientific, Inc.), anti-MKL1 (1:1,000; cat. no. Ab219981; Abcam), anti-Flag (1:1,000; cat. no. sc-166355; Santa Cruz Biotechnology, Inc.), anti-His (1:1,000; cat. no. 66005-1-Ig; Proteintech Group, Inc.),

(2) L1CAM Promotes Human Endometrial Cancer Via NF-κB ActivationCancers (Basel)January 8, 2026Hiroyuki Kurosu, Hiroshi Asano, Alaa-eldin Salah-eldin, Kazuya Hamada, Shugo Tanaka, Asuka Ishii, Issei Kawakita, Kentaro Kumagai, Kensuke Nakazono, Yuko Katayama, et al.(Santa Cruz Biotechnology, Dallas, TX, USA; sc-1647, 1:1000), OctA-Probe(H-5) (Santa Cruz Biotechnology, sc-166355, 1:1000) and GAPDH (FUJIFILM Wako Pure Chemical Corp, #015-25473, 1:10,000). 2.5. Cell Proliferation

(3) Extracellular Domain Shedding of TROP2 Activates EGFR Signaling to Drive Prostate Cancer MetastasisCancer researchDecember 1, 2025Liu S, Hsu EC, Aslan M, Garcia-Marques F, Shen M, Hartono AB, Solano F, Le K, Hwang H, Lee CS, et al.Santa Cruz Biotechnology Cat# sc-293133, RRID:AB_2861149; OctA-Probe, 1:1000, Santa Cruz Biotechnology Cat# sc-166355, RRID:AB_2017593; anti-AR, 1:1000, Santa Cruz Biotechnology Cat# sc-7305, RRID:AB_626671; anti-PI3K,

(4) Structural basis for the recognition and ubiquitylation of type-2 N-degron substrate by PRT1 plant N-recogninNat CommunAugust 21, 2025Woo Seok Yang, Seu Ha Kim, Minsang Kim, Hejeong Shin, Juyeon Lee, Alexander Sandmann, Ohkmae K. Park, Nico Dissmeyer, Hyun Kyu Song(or over 12 h) at 30 °C. Ubiquitylated Tyr61-BB was detected using an HRP-conjugated anti-FLAG tag (sc-166355; Santa Cruz Biotechnology, Dallas, TX, USA, 1:2000 ratio) and anti-Ub antibodies (sc-8017; Santa Cruz Biotechnology

(5) Exome Sequencing Reveals the Genetic Architecture of Non‐syndromic Orofacial Clefts and Identifies BOC as a Novel Causal GeneAdvanced ScienceAugust 1, 2025Qing He, Min Yu, Yuhua Jiao, Yizhu Xu, Xuqin Liang, Wenbin Huang, Linping Xu, Yuxia Hou, Zhanping Ren, Beile Lyu, et al.media containing ECD of WT or mutant BOC overnight at 4 °C with an anti‐FLAG antibody (Santa Cruz, #sc‐166355) and protein A/G PLUS‐Agarose beads (Santa Cruz, #sc‐2003). The beads were washed with PBS containing

(6) Structural insights into a highly flexible zinc finger module unravel INSM1 function in transcription regulationNat CommunMarch 4, 2025Heng Zhou, Xiaoling He, Yue Xiong, Yixuan Gong, Yuanyuan Zhang, Shuangli Li, Rui Hu, Ying Li, Xu Zhang, Xin Zhou, et al.(Santa Cruz, Sc-393976), anti-CTCF (G-8) (Santa Cruz, Sc-271474), and anti-Flag/OctA-Probe (H-5) (Santa Cruz, Sc-166355). The bands were visualized through chemiluminiscence using the BeyoECL Moon kit (Beyotime, P0018FS-2),

(7) Targeting WDPF domain of Hsp27 achieves a broad spectrum of antiviralMedComm (2020)March 1, 2025Wu M, Li W, Leung H, Wang Y, Wan Q, Chen P, Chen C, Li Y, Yao X, He ML.(GTX132339; GeneTex), Actin (sc‐47778; Santa Cruz)), hnRNP A1 (sc‐32301; Santa Cruz)), OctA–Probe (Flag, sc‐166355; Santa Cruz)), HSV‐1 gD (sc‐21719; Santa Cruz), HCoV‐OC43 N (40643‐T62; Sino), or GAPDH (sc‐32233; Santa Cruz

(8) PTH1R Suppressed Apoptosis of Mesenchymal Progenitors in Mandibular GrowthInternational Journal of Molecular SciencesNovember 24, 2024Chen Cui, Chuang Lu, Yanling Cai, Yuhua Xiong, Yihong Duan, Kaiwen Lan, Yi Fan, Xuedong Zhou, Xi Weimembrane was incubated with anti-IP3R3 (1:2000, BD Biosciences, 610312), FLAG (1:1000, Santa Cruz, sc-166355) and β-actin (1:1000, SAB, 41517) overnight. Incubated with Goat anti-rabbit or mouse IgG secondary

(9) TRIM28 Regulates Proliferation of Gastric Cancer Cells Partly Through SRF/IDO1 AxisJournal of CancerJune 11, 2024Zhiye Huang, Jiaxing Dong, Taohua Guo, Wanju Jiang, Renhao Hu, Shun Zhang, Tao Du, Xiaohua Jiangwas achieved with ECL chemiluminescence (BioRad, CA, USA). TRIM28 antibody (sc-33186), Flag antibody (sc-166355) was obtained from Santa Cruz (TX, USA). IDO1 antibody (#86630), SRF antibody (#5147), GAPDH antibody,

(10) Properties and Functional Analysis of Two Chorismate Mutases from Maritime PineCellsMay 28, 2024Fernando de la Torre, Beatriz Medina-Morales, Irene Blanca-Reyes, M. Belén Pascual, Concepción Ávila, Francisco M. Cánovas, Vanessa Castro-Rodríguezelectrophoresis in 10% SDS-PAGE and analyzed by western blot analysis using anti-Flag (1:1000, OctA-Probe H5, sc-166355, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or anti-GFP (1:1000; sc-9996, Santa Cruz Biotechnology,

(11) Inhibitory Effects of Latilactobacillus curvatus BYB3 Cell-Free Extract on Human Melanoma B16F10 Cells and Tumorigenic MiceJournal of Microbiology and BiotechnologyMarch 28, 2024Dingyun Li, Xing Wang, Dong-June Park, Dong Hun Lee, Sejong Oh(1:500, AF-401-NA, R&D Systems), mouse cleaved caspase-1 (1:1000, AG-20B-0042, AdipoGen), FLAG (1:1000, sc-166355, Santa Cruz Biotechnology), Myc (1:1000, ab13836, Abcam), GAPDH (1:1000, sc-25778, Sigma). Mice Assay

(12) E3 ubiquitin ligase rififylin has yin and yang effects on rabbit cardiac transient outward potassium currents (I(to)) and corresponding channel proteinsThe Journal of biological chemistryMarch 1, 2024Kabakov AY, Roder K, Bronk P, Turan NN, Dhakal S, Zhong M, Lu Y, Zeltzer ZA, Najman-Licht YB, Karma A, et al.receptor (Novus Biologicals; NBP1-85741; 1:1000); mouse anti Oct-A (Flag; Santa Cruz Biotechnology; sc-166355; 1:300); mouse anti c-Myc (Santa Cruz Biotechnology; sc-40; 1:300); and mouse anti-tubulin (Cell Signaling Technology

(13) SUMOylation modulates eIF5A activities in both yeast and pancreatic ductal adenocarcinoma cellsCellular & Molecular Biology LettersJanuary 16, 2024Rocío Seoane, Tomás Lama-Díaz, Antonia María Romero, Ahmed El Motiam, Arantxa Martínez-Férriz, Santiago Vidal, Yanis H. Bouzaher, María Blanquer, Rocío M. Tolosa, Juan Castillo Mewa, et al.(sc179, Santa Cruz Biotechnology), anti-β-actin (sc4778, Santa Cruz Biotechnology), anti-OctA-Probe (sc166355, Santa Cruz Biotechnology), and anti-Flag-M2 HRP-conjugated (A8592, Sigma). Anti-Smt3 antibody was kindly

(14) p57(Kip2) acts as a transcriptional corepressor to regulate intestinal stem cell fate and proliferationCell reportsJune 27, 2023Creff J, Nowosad A, Prel A, Pizzoccaro A, Aguirrebengoa M, Duquesnes N, Callot C, Jungas T, Dozier C, Besson A.

(15) SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injuryiScienceJune 16, 2023Xia Q, Mao M, Zhan G, Luo Z, Zhao Y, Li X.anti-HA,Santa Cruz Biotechnology,Cat#sc-7392; RRID:AB_627809 Mouse anti-Flag,Santa Cruz Biotechnology,Cat#sc-166355; RRID:AB_2017593 Rabbit anti-Myc,Proteintech,Cat#16286-1-AP; RRID:AB_11182162 Rabbit anti-His,Sigma-Aldrich,Cat#SAB1306085; Rabbit

(16) A polycistronic transgene design for combinatorial genetic perturbations from a single transcript in DrosophilaPLoS geneticsJune 1, 2023Teague AG, Quintero M, Karimi Dermani F, Cagan RL, Bangi E.primary and secondary antibodies: mouse anti-OctA(FLAG) antibody (1:1000, Santa Cruz Biotechnology, sc-166355), rat anti-HA High-Affinity Antibody (1:1000, ROAHAHA, MilliporeSigma), rabbit anti-GFP N-terminal antibody

(17) PERIOD phosphorylation leads to feedback inhibition of CK1 activity to control circadian periodMolecular cellMay 18, 2023Philpott JM, Freeberg AM, Park J, Lee K, Ricci CG, Hunt SR, Narasimamurthy R, Segal DH, Robles R, Cai Y, et al.

(18) Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's diseaseCell reportsMarch 28, 2023Mansky RH, Greguske EA, Yu D, Zarate N, Intihar TA, Tsai W, Brown TG, Thayer MN, Kumar K, Gomez-Pastor R.(Santa Cruz sc-8432, RRID:AB_626630), H3 (Cell Signaling technology, 9715, RRID:AB_331563), FLAG (Santa Cruz, sc-166355, RRID:AB_2017593), GFP (Santa Cruz, sc-9996, RRID:AB_627695). Protein levels from immunoblots were quantified

(19) Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regenerationeLifeFebruary 3, 2023Cho E, Che X, Ang MJ, Cheon S, Lee J, Kim KS, Lee CH, Lee SY, Yang HY, Moon C, et al.,Anti-HA-Tag (mouse monoclonal),Santa Cruz,Cat#sc-7392,(1:1000) Antibody,Anti-Flag (OctA)-probe (mouse monoclonal),Santa Cruz,Cat#sc-166355,(1:1000) Antibody,Anti-lamin A/C (rabbit polyclonal),Cell Signaling Technology,Cat#2032,(1:1000) Antibody,Anti-lamin

(20) Extracellular Vesicles Are Conveyors of the NS1 Toxin during Dengue Virus and Zika Virus InfectionVirusesJanuary 27, 2023Safadi DE, Lebeau G, Lagrave A, Mélade J, Grondin L, Rosanaly S, Begue F, Hoareau M, Veeren B, Roche M, et al.ab41616), mouse anti-FLAG mAb (FLAG) (1:1000, Cell signaling technology, Danvers, MA, USA or 1:200, sc-166355, Santa-Cruz Biotechnology Inc., Santa Cruz, CA, USA), mouse anti-CD63 (1:200, sc-5275, Santa-Cruz Biotechnology Inc

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9/5/2018Good Oct-A Antibody for Cell Immunofluorescence Chiara ChiereghinThe OctA-Probe Antibody was used for immunofluorescence on HEK293 cells transiently transfected with a FLAG-fused protein, to evaluate the localisation of the FLAG-fused protein transfected.

OctA-Probe (H-5) Antibody

OctA-Probe (H-5) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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OctA-Probe (H-5) Antibody from Santa Cruz Biotechnology, Inc.

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