Anti-Ki67 antibody [SP6] ab16667 from Abcam

Product Specs
ItemAnti-Ki67 antibody [SP6]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab16667
Antibody TypePrimary
CloneSP6
IsotypeIgG
ApplicationsFlow Cytometry, Immunocytochemistry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Multiplex immunohistochemistry, Western blot, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Multiplex immunohistochemistry, Western blot, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Multiplex immunohistochemistry, Western blot
ClonalityMonoclonal
Quantity1 ML, 100 UL, 500 UL
HostRabbit
Conjugate/TagUnconjugated
Gene NameMKI67
ValidationKnockOut Validated
ReactivityHuman, Mouse, Rat
TargetKi67

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Mapk7 deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signalingGenes & DiseasesMarch 1, 2024Chengzhi Wu, Hengyu Liu, Dongmei Zhong, Xiaoming Yang, Zhiheng Liao, Yuyu Chen, Shun Zhang, Deying Su, Baolin Zhang, Chuan Li, et al.1:1000), anti-RUNX2 (Abcam, ab192256: 1:1000), anti-MMP13 (Abcam, ab19013; 1:1000), anti-Ki67 (Abcam, ab16667; 1:1000), anti-Bax (Abcam, ab182733; 1:1000), anti-caspase 3 (Abcam, ab184787; 1:1000), anti-PTEN (Cell

(2) SETDB1-mediated CD147-K71 di-methylation promotes cell apoptosis in non-small cell lung cancerGenes & DiseasesMarch 1, 2024Ming-Yan Shi, Yarong Wang, Ying Shi, Ruofei Tian, Xiaohong Chen, Hai Zhang, Ke Wang, Zhinan Chen, Ruo Chenwashed with PBS for 15 min, blocked using 10% goat serum for 20 min, and incubated with anti-Ki-67 (ab16667; Abcam, UK; dilution 1:200) and anti-Bcl2 (12789-1-AP; Proteintech, China; dilution 1:200) antibodies

(3) The MORC2 p.S87L mutation reduces proliferation of pluripotent stem cells derived from a patient with the spinal muscular atrophy-like phenotype by inhibiting proliferation-related signaling pathwaysNeural Regeneration ResearchJanuary 1, 2024Sen Zeng, Honglan Yang, Binghao Wang, Yongzhi Xie, Ke Xu, Lei Liu, Wanqian Cao, Xionghao Liu, Beisha Tang, Mujun Liu, et al.and subjected to immunofluorescence staining with an anti-Ki-67 antibody (human, 1:250, Abcam, Cat# ab16667, RRID: AB_302459) at 4°C for 12 hours. The iPSCs or MNPs were then incubated with a goat anti-Rabbit

(4) Nanomicelles for GLUT1-targeting hepatocellular carcinoma therapy based on NADPH depletionDrug DeliveryDecember 31, 2023Congyi Zhang, Zehui Liu, Feng Wang, Bin Zhang, Xirui Zhang, Peiwen Guo, Tianwei Li, Sheng Tai, Changmei Zhangwere approved by animal ethics committee of Harbin Medical University (KY2019-046). Ki67 antibody (ab16667), second antibody goat anti-rabbit IgG (BA-1000) were purchased form Abcam. Liquid mass spectrometer

(5) CSTF2T facilitates pancreatic adenocarcinoma growth and metastasis by elevating H3K4Me1 methylation of CALB2 via ASH2LCancer Biology & TherapyDecember 31, 2023Wei Liu, Dongwei Hu, Jinjie Li, Qigang Xu, Zongjing Chen, Bo Xuexcess liquid shaken off. The sections were probed with primary antibodies (1:200, Abcam) against Ki-67 (ab16667) and PCNA (ab92552) overnight at 4°C. After three PBS washes, the sections were probed at room temperature

(6) ANKS1A-Deficiency Aberrantly Increases the Entry of the Protein Transport Machinery into the Ependymal CiliaMol CellsDecember 31, 2023Haeryung Lee, Jiyeon Lee, Miram Shin, Soochul Parkdifferentiation day, the cellular staining was performed with the following antibodies: rabbit IgG anti-Ki67 (ab16667, 1:200; Abcam, UK), mouse IgG1 anti-S100β (ab11178, 1:200; Abcam), mouse IgG2b anti-AcTub (T7451, 1:500;

(7) Isoalantolactone suppresses gallbladder cancer progression via inhibiting the ERK signalling pathwayPharmaceutical BiologyDecember 31, 2023Xingyu Lv, Yuqi Lin, Xi Zhu, Xiujun Caiparaffin. Paraffin-embedded samples were cut at 5 μm thickness. Immunohistochemical staining for Ki-67 (ab16667, Abcam, Cambridge, UK, 1:200) was performed using a DAB detection kit (GK600710, Gene Company, Shanghai,

(8) Deletion of adipocyte NOS3 potentiates high-fat diet-induced hypertension and vascular remodelling via chemerinCardiovascular ResearchDecember 30, 2023Andy W C Man, Yawen Zhou, Gisela Reifenberg, Alica Camp, Thomas Münzel, Andreas Daiber, Ning Xia, Huige Lisolution to inhibit peroxidase activity. The sections were incubated with primary antibody of Ki-67 (#ab16667, Abcam, 1:50) in 4°C overnight. After washing twice in phosphate buffered saline with 0.1% Tween 20

(9) OMA1 and YME1L as a Diagnostic Panel in Hepatocellular CarcinomaYale J Biol MedDecember 29, 2023Shimaa A. Abass, Nabil Mohie Abdel-Hamid, Ahmed M. Elshazly, Walied Abdo, Sherin Zakariasections were incubated with anti-rat ki67 monoclonal antibody (1:200 dilution, Abcam Inc. Cat No. ab16667). They were also incubated with horseradish peroxidase conjugate anti-Rabbit antibody (Abcam Inc. Cat

(10) USP7 inhibits the progression of nasopharyngeal carcinoma via promoting SPLUNC1-mediated M1 macrophage polarization through TRIM24Cell Death DisDecember 21, 2023Huai Liu, Ling Tang, Sha Gong, Tengfei Xiao, Hongmin Yang, Wangning Gu, Hui Wang, Pan Chenantibodies against SPLUNC1 (1:50, 10413-1-AP, Proteintech), CD86 (1:100, ab220188, Abcam), Ki67 (1:50, ab16667, Abcam), TRIM24 (1:200, ab70560, Abcam), and USP7 (1:100, ab264422, Abcam) overnight at 4 °C, followed

(11) GRB7 plays a promoting role in the progression of gastric cancerBMC CancerDecember 21, 2023Guomin Zhu, Hu Cai, Qiang Xiao, Shukun Zeng, Xiaohua Jiang, Donglan Liuprimary antibody anti- GRB7 (1:100, Cat. SRP06265, Saierbio) or anti-Ki-67 antibody (1:200, Cat. # Ab16667, Abcam) at 4℃ overnight. In the next day, sections were washed 3 times for 5 min in 1xPBST, followed

(12) Neoadjuvant durvalumab plus radiation versus durvalumab alone in stages I-III non-small cell lung cancer: survival outcomes and molecular correlates of a randomized phase II trialNat CommunDecember 19, 2023Nasser K. Altorki, Zachary H. Walsh, Johannes C. Melms, Jeffery L. Port, Benjamin E. Lee, Abu Nasar, Cathy Spinelli, Lindsay Caprio, Meri Rogava, Patricia Ho, et al.(F7.2.38), Dako #M7524; CD4 (EPR6855), Abcam ab181724; CD8 (C8/144B), Dako #M7103; Ki67 (SP6), Abcam #ab16667; PD-1 (EPR4877(2)), Abcam ab186928; PD-L1 (SP142), ab236238, panCK (PCK-26) Sigma-Aldrich #C5992. Stained

(13) Microgravity triggers ferroptosis and accelerates senescence in the MG-63 cell model of osteoblastic cellsNPJ MicrogravityDecember 16, 2023Nancy Garbacki, Jérôme Willems, Thibaut Neutelings, Charles Lambert, Christophe Deroanne, Astrid Adrian, Markus Franz, Matthias Maurer, Philippe De Gieter, Betty Nusgens, et al.incubations times and washings. The following primary antibodies were used: rabbit anti-KI67 (1:250, Abcam #ab16667), mouse anti-α-tubulin (1:1000, Sigma, #T6199), rabbit anti-acetyl-α-tubulin (1:500, Cell Signaling

(14) Steroidogenesis and androgen/estrogen signaling pathways are altered in in vitro matured testicular tissues of prepubertal miceElifeDecember 14, 2023Laura Moutard, Caroline Goudin, Catherine Jaeger, Céline Duparc, Estelle Louiset, Tony Pereira, François Fraissinet, Marion Delessard, Justine Saulnier, Aurélie Rives-Feraille, et al.(rabbit polyclonal),Proteintech,17909–1-AP,WB (1:1000) Antibody,Anti-Ki67 (rabbit monoclonal),Abcam,ab16667,IF (1:100) Antibody,Anti-mouse Alexa 488 (goat),Abcam,ab150113,IF (1:200) Antibody,Anti-rabbit Alexa

(15) Iberverin exhibits antineoplastic activities against human hepatocellular carcinoma via DNA damage-mediated cell cycle arrest and mitochondrial-related apoptosisFront PharmacolDecember 13, 2023Yuting Zhang, Jiao Du, Libo Jin, Liying Pan, Xiufeng Yan, Sue Linnormal goat serum for 15 min, and then incubated with anti-Ki-67 (Abcam, the United Kingdom, catalog no. ab16667), anti-PCNA (Proteintech, the United States, catalog no. 10205-2-AP) and anti-Cleaved Caspase-3 (Cell Signaling

(16) Leptin secreted by adipocytes promotes EMT transition and endometrial cancer progression via the JAK2/STAT3 signalling pathwayAdipocyteDecember 13, 2023Lifan Shen, Chen Zhang, Kaiying Cui, Xin Liang, Genhai Zhu, Lan Hongprimary antibodies against JAK2 (#cat: ab32101, Abcam), STAT3 (#cat: ab68153, Abcam), Ki-67 (#cat: Ab16667, Abcam), and PCNA (the primary antibody against ab29, Abcam), at 4°C overnight, and then washed with

(17) PD-L1 blockade TAM-dependently potentiates mild photothermal therapy against triple-negative breast cancerJ NanobiotechnologyDecember 11, 2023Chao Wang, Yong-Hong Xu, Hua-Zhen Xu, Ke Li, Quan Zhang, Lin Shi, Li Zhao, Xiao Chenstaining. IHC analysis Antibodies for immunohistochemical analysis included PCNA (ab92552, Abcam), Ki67 (ab16667, Abcam), cle-caspase 3 (9664, CST), PD-L1 (66248-1-Ig, Proteintech), CD206 (ab64693, Abcam), CD80 (BS-2211R,

(18) Multisite Is Superior to Single-Site Intratumoral Chemotherapy to Retard the Outcomes of Pancreatic Ductal Adenocarcinoma in a Murine ModelCancers (Basel)December 11, 2023Janette Lazarovits, Ron Epelbaum, Jesse Lachter, Yaron Amikam, Jacob Ben Arietechniques were employed to assess the expression levels of specific biomarkers: Ki67 staining (ABCAM cat#ab16667, Cambridge, UK) as a marker of proliferation, TUNEL assay (S7100 manual kit by Merck Millipore, Beijing,

(19) Single-cell map of dynamic cellular microenvironment of radiation-induced intestinal injuryCommun BiolDecember 9, 2023Hao Lu, Hua Yan, Xiaoyu Li, Yuan Xing, Yumeng Ye, Siao Jiang, Luyu Ma, Jie Ping, Hongyan Zuo, Yanhui Hao, et al.peroxidase. Slides were incubated with goat serum for 10 min and then with the primary antibody anti-Ki67 (#ab16667, 1:200; Abcam). A horse radish peroxidase (HRP)-based signal amplification system was then hybridized

(20) Integrin-αvβ3 is a therapeutically targetable fundamental factor in medulloblastoma tumorigenicity and radioresistanceCancer Research CommunicationsDecember 7, 2023William Echavidre, Jerome Durivault, Célia Gotorbe, Thays Blanchard, Marina Pagnuzzi, Valérie Vial, Florian Raes, Alexis Broisat, Rémy Villeneuve, Régis Amblard, et al.Pharmingen) and monoclonal anti-mouse α-smooth muscle actin (αSMA; A2547, 1:1,000; Sigma) or anti-Ki67 (ab16667, 1:500; Abcam) antibodies diluted in TBS containing 1% BSA at 1:100 overnight at room temperature, and

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(1)

1/1/2024Rabbit Anti-Ki67 Antibody From Abcam is AverageDr. David SunnenThe specific goal of this experiment was to evaluate the effects of the drug on mouse fibroblast proliferation. We wanted to use a Ki-67 rabbit antibody. This rabbit antibody provided fair staining ... Read Review
(1)

2/3/2016Review for Anti-Ki67 Antibody Liyong Z.We injected adipose-derived stem cells into mice to detect the wound healing effects and signaling cascade involved. Several markers are chosen including alpha smooth muscle, Cytokeratin 10, Ki67, and... Read Review

Anti-Ki67 antibody [SP6] from Abcam

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Anti-Ki67 antibody [SP6] from Abcam

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