Review for Anti-Ki67 Antibody

Surgery
University of Pittsburgh
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Ki67 antibody

Catalog Number:

ab16667

We injected adipose-derived stem cells into mice to detect the wound healing effects and signaling cascade involved. Several markers are chosen including alpha smooth muscle, Cytokeratin 10, Ki67, and Wnt11. We chose Abcam antibodies since the company has a good reputation of good quality.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse prostate

Primary Incubation

4 degrees celcius overnight

Blocking Agent

10% normal goat non-immune serum

Secondary Incubation

Goat anti-rabbit biotinylated secondary antibody

Tertiary Incubation

Vectastain ABC reagent

Detection

3,3’-diaminobenzidine

Results Summary

Staining fine, relatively weak

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works well

The Bad

NA

The Bottom Line

Works

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