Fig 2: Depletion of ABL1 increases the apoptosis of CRC cells. Flow cytometry detected apoptosis of SW480 (A) and HCT-116 (B) cells infected with shCtrl or shABL1. Cells without infection was used as control (Con). (C) Quantification of apoptotic cells. Data are shown as mean ± SD. ***p < 0.001 compared with control. (D) Western blot analysis of Bax, Bcl-xl, and Bcl-2 expression in CRC cells infected with shCtrl or shABL1. Cells without infection was used as control (Con).

Fig 3: Cordifoliketones A affects the expression of apoptosis-associated proteins in AsPC-1 cells. (A) Representative western blot images and (B) quantification of protein expression levels of Bax, Bad, Bcl-xL, Bcl-2, caspase-3, caspase-8 and caspase-9 following cordifoliketones A treatment (0, 2, 4, and 6 µg/ml). Average values are from three independent experiments performed in duplicate. Data are presented as the mean ± standard deviation (n=3). Bax, Bcl-2-associated X protein; Bad, Bcl-2-associated death promoter; Bcl-XL, B-cell lymphoma extra-large; Bcl-2, B-cell lymphoma 2.

Fig 4: Cordifoliketones A affects the expression of apoptosis-associated proteins in PANC-1 cells. (A) Representative western blot images and (B) quantification of protein expression levels of Bax, Bad, Bcl-xL, Bcl-2, caspase-3, caspase-8 and caspase-9 following cordifoliketones A treatment (0, 2, 4, and 6 µg/ml). Average values are from three independent experiments performed in duplicate. Data are presented as the mean ± standard deviation (n=3). Bax, Bcl-2-associated X protein; Bad, Bcl-2-associated death promoter; Bcl-XL, B-cell lymphoma extra-large; Bcl-2, B-cell lymphoma 2.

Fig 5: Cordifoliketones A affects the expression of apoptosis-associated proteins in BxPC-3 cells. (A) Representative western blot images and (B) quantification of protein expression levels of Bax, Bad, Bcl-xL, Bcl-2, caspase-3, caspase-8 and caspase-9 following cordifoliketones A treatment (0, 2, 4, and 6 µg/ml). Average values are from three independent experiments performed in duplicate. Data are presented as the mean ± standard deviation (n=3). Bax, Bcl-2-associated X protein; Bad, Bcl-2-associated death promoter; Bcl-XL, B-cell lymphoma extra-large; Bcl-2, B-cell lymphoma 2.