Fig 2: The production of TSLP by keratinocytes was increased in Stat3 cKO mice, which recruited more target cells in AD-like skin inflammation. (A) Analysis of TSLP expression level (pg/ml) in the ear skin of Stat3f/f mice and Stat3 cKO mice after DNCB or vehicle induction by ELISA (n = 3-4). (B) IF staining showed that TSLP was primarily expressed in keratinocytes (red). Bar = 50 μm. (C) IF staining revealed that the infiltration of TSLPR+ cells (green), CD11c+ cells (red), and CD68+ cells (red) was increased in the dermis of DNCB-treated Stat3 cKO mice compared with Stat3f/f mice. (D) Statistical analysis of the abovementioned cells (n = 3-4). The data are presented as the mean ± SD; P > 0.05 denotes ns; *P < 0.05, **P < 0.01, and ***P < 0.001.