Fig 1: IL-10 and protection from hypertriglyceridemia are necessary for fluoxetine to sustain cardiac glucose oxidation during sepsis.(A and B) U-13C glucose tracing (A) experimental protocol and (B) labeling strategy. (C and D) U-13C glucose labeling of at 7 hours post-infection. (C) Citrate labeling fraction. (D) Pyruvate labeling fraction. n = 7 per condition, one experiment shown. (E) Western blot of pyruvate dehydrogenase (PDH) complex, phospho-PDH, and cyclophilin A of hearts at 8 to 10 hours post-infection. n = 2 to 3 per condition, representative of three independent experiments. (F) Cardiac Pdk4 transcript levels at 10 hours post-infection. n = 5 to 10 per condition, two replicates combined. (G to H) U-13C glucose labeling at 7 hours post-infection of hearts from mice injected with isotype control or anti-IL10R. (G) Citrate labeling fraction. (H) Pyruvate labeling fraction. n = 7 per condition, one experiment shown. (I) Western blot of PDH, phospho-PDH, and cyclophilin A of hearts at 8 to 10 hours post-infection. n = 2 to 3 per condition, representative of two independent experiments. Lower right corner of gel ripped after running. The lower right corner and rest of gel were pieced together for transferring. (J and K) U-13C glucose labeling at 7 hours post-infection in hearts from mice injected with pluronic or water. (J) Citrate labeling fraction. (K) Pyruvate labeling fraction. n = 6 per condition, one experiment shown. (L) Western blot of PDH, phospho-PDH, and cyclophilin A of hearts at 8 to 10 hours post-infection mice injected with water or Pluronic F-127 at the time of infection. n = 2 to 3 per condition, representative of two independent experiments. (M) Circulating BNP levels at 8 to 10 hours post-infection. n = 5 to 15 per condition, three independent experiments combined. (N) Circulating BNP levels at 8 to 10 hours post-infection in mice injected with water or Pluronic. n = 5 to 15 per condition, three independent experiments combined. (O) Model schematic. Two-way ANOVA with Tukey’s multiple comparisons. Uncropped blots are shown in figs. S6 and S7. In all panels, data represent means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.