Spectral flow cytometers capture the full fluorescence spectrum of labeled cells and particles, unlike conventional cytometers, which rely on optical filters to isolate specific wavelengths. Multiple specialized detectors enable the identification of multiple fluorophores simultaneously based on unique spectral fingerprints. Spectral unmixing, analogous to compensation in conventional cytometry, is used to distinguish fluorophores with even closely overlapping emission spectra. As a result, spectral cytometers can accommodate larger, more complex panels, enhancing multiparametric analysis. Selecting a spectral cytometer requires balancing cost with experimental needs, considering factors such as laser count, detector sensitivity, sample format, acquisition speed, and throughput. These specifications directly impact the resolution and efficiency of high-parameter experiments. Consult the instrument manufacturer for more specifications and features.
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- 349 nm, 405 nm, 445 nm, 488 nm, 532 nm, 561 nm, 594 nm, 640 nm, 785 nm
- Up to 60 parameters, including FSC and SSC
- Sample Input: 1.5 mL, 5 mL, and 15 mL
- Excitation lasers: 349nm, 405 nm, 488 nm, 561 nm, 637 nm, 781 nm
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