Spectral flow cytometers capture the full fluorescence spectrum of labeled cells and particles, unlike conventional cytometers, which rely on optical filters to isolate specific wavelengths. Multiple specialized detectors enable the identification of multiple fluorophores simultaneously based on unique spectral fingerprints. Spectral unmixing, analogous to compensation in conventional cytometry, is used to distinguish fluorophores with even closely overlapping emission spectra. As a result, spectral cytometers can accommodate larger, more complex panels, enhancing multiparametric analysis. Selecting a spectral cytometer requires balancing cost with experimental needs, considering factors such as laser count, detector sensitivity, sample format, acquisition speed, and throughput. These specifications directly impact the resolution and efficiency of high-parameter experiments. Consult the instrument manufacturer for more specifications and features.
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- 349 nm: 30 mW, 405 nm: 50 mW, 488 nm: 100 mW, 561 nm: 50 mW, 637 nm: 100 mW
- 78 fluorescence channels, 8 scatter and imaging channels
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- 78 fluorescence channels, 8 scatter and imaging channels
- 355 nm - 60 mW 405 nm - 200 mW 488 nm - 150 mW 561 nm - 150 mW 637 nm - 140 mW
- 50 detection parameters (including FSC and SSC) featuring cascadegon arrays with 48 fluorescent ...
- 5.0-mL polystyrene and polypropylene tubes, optional high throughput sampler to acquire samples from...
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