CHO Cell Lines

CHO Cell Lines The CHO cell line is derived from the Chinese hamster ovary and has become a staple source of cells due to their robust growth as adherent cells or in suspension. They are amenable to genetic modifications and methods for cell transfection, recombinant protein expression, and clone selection are well characterized. CHO cells can undergo human-compatible post-translational modifications, making them ideal for therapeutic and pharmaceutical applications like protein manufacturing. CHO cells lack proline synthesis abilities and do not express EGFR. Various variant cell lines have emerged from CHO, such as CHO-K1 which lack dihydrofolate reductase (DHFR) activity. CHO cells with genetic modifications, such as gene overexpression or knockouts have been generated for research use. Visit the cell supplier for more information.

CHO Cell Lines

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Recent Product Review

  • Excellent And Cheap Anti-Alpha Tubulin Antibody

    This antibody was used to perform immunofluorescence microscopy on HeLa and CHO cells. The antibody was specific and showed clear immunoreactive staining that is similar to more expensive anti-tubulin antibodies.

  • Western blot with HMGB1 Antibody in CHO Cells

    This antibody was used to monitor equal protein loading in all wells (house keeping gene control). Methodology: CHO cell lysates were prepared in RIPA lysis buffer. 50 µg of clarified lysate was loaded on a 10% Tris-Glycine gel and SDS-PAGE was performed. Proteins were transferred onto a nitrocellulose membrane using semi-dry transfer method. Ponceau staining was done to visualize proper protein transfer. The membrane was blocked in blocking buffer (Odyssey Blocking Buffer) for 30 min at room temperature. HMGB1 antibody was diluted (1:1000 dilution) in blocking buffer and incubated with the membrane for 1 hr at room temperature. Membrane was washed thrice in wash buffer (1xTBS-T (0.1%)) and then incubated with secondary antibody – LI-COR IRDye 800 (1:5000 dilution) for 1 hr at room temperature. Membrane was washed thrice in wash buffer and the signal was captured on a LI-COR Odyssey imager. Rating: Very good antibody with low signal/noise ratio