Size exclusion (SEC) HPLC columns contain porous stationary phase particles that trap and gradually release biomolecules according to their size. Large molecules pass through the SEC column first, while smaller molecules that can diffuse through the particles elute last. SEC HPLC, which can operate under high pressure, allows for fast, efficient purification of biomolecules such as proteins and nucleic acids. Consider column length, diameter, and particle size when browsing for HPLC columns.
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- Anion Exchange, Cation Exchange, Hydrophilic Interaction, Reversed Phase, Size Exclusion, C18
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