
In vitro transcription kits are used for the generation of large amounts of high-quality RNA from a DNA template, utilizing a straightforward workflow and ready-to-use reagents. In vitro transcription reactions are cell-free and generally require a linear DNA template with a promoter upstream of the sequence of interest, primers, ribonucleotide triphosphates, a buffer system consisting of DTT and magnesium ions, and an RNA polymerase enzyme. In vitro transcription kits are used for a variety of applications such as cell-free protein synthesis, RNAi analysis, RNA synthesis, hybridization, RNA-binding studies, and more. Browse in vitro transcription kits below and compare between different suppliers. As kit contents and methodology may vary, visit the supplier pages for more information.
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